Restriction map for
pFN21AE5588
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbsI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrI
BssHII Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MslI MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TfiI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcsI 127 AAATTC [GA]^AATT[CT]
AcyI 774 GGCGCC G[GA]^CG[CT]C
AgeI 1007 ACCGGT A^CCGGT
AlwI 879 GATCC GGATC(4/5)
AocI 714 CCTGAGG CC^T[ACGT]AGG
ApoI 127 AAATTC [GA]^AATT[CT]
AspHI 571 GAGCTC G[AT]GC[AT]^C
BalI 593 TGGCCA TGG^CCA
BbeI 774 GGCGCC GGCGC^C
BbrPI 547 CACGTG CAC^GTG
BbvCI 140 CCTCAGC CCTCAGC(-5/-2)
BclI 236 TGATCA T^GATCA
BfaI 292 CTAG C^TAG
BsaHI 774 GGCGCC G[GA]^CG[CT]C
BsaWI 1007 ACCGGT [AT]^CCGG[AT]
BsiEI 1005 CGACCG CG[GA][CT]^CG
BsiHKAI 571 GAGCTC G[AT]GC[AT]^C
Bsp1407I 188 TGTACA T^GTACA
BspMI 490 ACCTGC ACCTGC(4/8)
BsrGI 188 TGTACA T^GTACA
BssSI 634 CTCGTG CACGAG(-5/-1)
BstYI 878 AGATCC [GA]^GATC[CT]
Bsu36I 714 CCTGAGG CC^T[ACGT]AGG
CfoI 775 GCGC GCG^C
Eco81I 714 CCTGAGG CC^T[ACGT]AGG
FbaI 236 TGATCA T^GATCA
HaeII 774 GGCGCC [GA]GCGC^[CT]
HhaI 775 GCGC GCG^C
Hin1I 774 GGCGCC G[GA]^CG[CT]C
HinP1I 775 GCGC G^CGC
KasI 774 GGCGCC G^GCGCC
MaeI 292 CTAG C^TAG
MflI 878 AGATCC [GA]^GATC[CT]
MluNI 593 TGGCCA TGG^CCA
MscI 593 TGGCCA TGG^CCA
MseI 5 TTAA T^TAA
NarI 774 GGCGCC GG^CGCC
PinAI 1007 ACCGGT A^CCGGT
PmaCI 547 CACGTG CAC^GTG
PmlI 547 CACGTG CAC^GTG
PshAI 625 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 205 CTGCAG CTGCA^G
SacI 571 GAGCTC GAGCT^C
ScaI 164 AGTACT AGT^ACT
SfcI 205 CTGCAG C^T[GA][CT]AG
SfoI 774 GGCGCC GGC^GCC
SmaI 820 CCCGGG CCC^GGG
SmlI 946 CTCAAG C^T[CT][GA]AG
SspBI 188 TGTACA T^GTACA
Tru9I 5 TTAA T^TAA
Tsp509I 128 AATT ^AATT
TspRI 495 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 539 CCAAGCTCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 878 AGATCC [GA]^GATC[CT]
XmaCI 820 CCCGGG C^CCGGG
XmaI 820 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 313 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
799 CAGCAGCTG
BanI 119 GGCACC G^G[CT][GA]CC
774 GGCGCC
BpmI 840 CTCCAG CTGGAG(16/14)
955 CTGGAG
BsaAI 547 CACGTG [CT]AC^GT[GA]
664 TACGTG
BsaI 631 GGTCTC GGTCTC(1/5)
837 GGTCTC
BsrFI 938 ACCGGC [GA]^CCGG[CT]
1007 ACCGGT
BstEII 486 GGTCACC G^GT[ACGT]ACC
1074 GGTCACC
BstPI 486 GGTCACC G^GT[ACGT]ACC
1074 GGTCACC
Cfr10I 938 ACCGGC [GA]^CCGG[CT]
1007 ACCGGT
DraIII 240 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
852 CACCAAGTG
DsaI 924 CCACGG C^C[GA][CT]GG
1109 CCGTGG
EaeI 593 TGGCCA [CT]^GGCC[GA]
600 CGGCCA
EcoO65I 486 GGTCACC G^GT[ACGT]ACC
1074 GGTCACC
EcoT14I 671 CCTTGG C^C[AT][AT]GG
971 CCAAGG
HinfI 22 GACTC G^A[ACGT]TC
263 GACTC
PflMI 798 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
924 CCACGGGCTGG
PleI 22 GACTC GAGTC(4/5)
263 GACTC
StyI 671 CCTTGG C^C[AT][AT]GG
971 CCAAGG
Van91I 798 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
924 CCACGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BanII BbvI BcnI BmyI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I TthHB8I