Restriction map for
pFN21AE5463
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BciVI
BclI BcnI BfrI BglI BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstNI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRII EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvaI MvnI MwoI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
ScrFI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AlwNI 282 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 666 GGTCC G^G[AT]CC
BbsI 778 GTCTTC GAAGAC(2/6)
BbvCI 303 CCTCAGC CCTCAGC(-5/-2)
BbvI 627 GCAGC GCAGC(8/12)
BcgI 792 CGAAGCTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 119 AGATCT A^GATCT
BpmI 159 CTCCAG CTGGAG(16/14)
BpuAI 778 GTCTTC GAAGAC(2/6)
BsaJI 633 CCATGG C^C[ACGT][ACGT]GG
BsmI 802 GCATTC GAATGC(1/-1)
BsrDI 511 GCAATG GCAATG(2/0)
BstEII 534 GGTAACC G^GT[ACGT]ACC
BstPI 534 GGTAACC G^GT[ACGT]ACC
Cac8I 291 GCTTGC GC[ACGT]^[ACGT]GC
DraII 402 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 633 CCATGG C^C[GA][CT]GG
EcoO109I 402 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 534 GGTAACC G^GT[ACGT]ACC
EcoRV 576 GATATC GAT^ATC
EcoT14I 633 CCATGG C^C[AT][AT]GG
Fnu4HI 627 GCAGC GC^[ACGT]GC
HaeIII 404 GGCC GG^CC
HindIII 677 AAGCTT A^AGCTT
ItaI 627 GCAGC GC^[ACGT]GC
MslI 732 CATAATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 633 CCATGG C^CATGG
NlaIV 402 GGGGCC GG[ACGT]^[ACGT]CC
NspI 885 GCATGT [GA]CATG^[CT]
PflMI 95 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 759 GAGTC GAGTC(4/5)
SfaNI 624 GATGC GCATC(5/9)
StyI 633 CCATGG C^C[AT][AT]GG
TseI 627 GCAGC G^C[AT]GC
Tsp45I 691 GTGAC ^GT[GC]AC
TspRI 144 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 95 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 29 CCATTCTAATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 516 GTAC GT^AC
894 GTAC
AlwI 652 GATCC GGATC(4/5)
643 GGATC
BfaI 299 CTAG C^TAG
544 CTAG
BseRI 366 GAGGAG GAGGAG(10/8)
504 GAGGAG
BsiYI 95 CCATTGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
834 CCTGATTTGGG
BslI 95 CCATTGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
834 CCTGATTTGGG
BsmAI 111 GAGAC GTCTC(1/5)
157 GTCTC
BsrI 287 ACTGG ACTGG(1/-1)
896 ACTGG
BstF5I 540 CATCC GGATG(2/0)
831 CATCC
Cfr13I 403 GGGCC G^G[ACGT]CC
666 GGTCC
FokI 540 CATCC GGATG(9/13)
831 CATCC
HphI 390 GGTGA GGTGA(8/7)
900 GGTGA
MaeI 299 CTAG C^TAG
544 CTAG
RsaI 516 GTAC GT^AC
894 GTAC
Sau96I 403 GGGCC G^G[ACGT]CC
666 GGTCC
SmlI 322 CTCAAG C^T[CT][GA]AG
647 CTCAAG
TfiI 213 GAATC G^A[AT]TC
447 GAATC
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
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AcsI AluI ApoI BstYI DdeI
DpnII HinfI MaeIII MboI MboII
MflI MnlI MseI NdeII NlaIII
Sau3AI Tru9I Tsp509I XhoII