Restriction map for
pFN21AE5340
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII BalI BamHI
BanII BbeI BbsI BbvCI BcgI
BcnI BglI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BspEI BspLU11I BspMI BsrBI BssHII
Bst1107I BstBI BstUI BstZ17I CelII
CfoI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoRI EcoRV
EcoT22I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 291 GAATTT [GA]^AATT[CT]
AflII 154 CTTAAG C^TTAAG
ApoI 291 GAATTT [GA]^AATT[CT]
AvaI 716 CCCGAG C^[CT]CG[GA]G
AvrII 564 CCTAGG C^CTAGG
BanI 599 GGCACC G^G[CT][GA]CC
BbrPI 625 CACGTG CAC^GTG
BciVI 728 GTATCC GTATCC(6/5)
BclI 581 TGATCA T^GATCA
BfaI 565 CTAG C^TAG
BfrI 154 CTTAAG C^TTAAG
BglII 383 AGATCT A^GATCT
BlnI 564 CCTAGG C^CTAGG
BmyI 393 GGGCAC G[AGT]GC[ACT]^C
BpmI 551 CTGGAG CTGGAG(16/14)
BsaAI 625 CACGTG [CT]AC^GT[GA]
BsoBI 716 CCCGAG C^[CT]CG[GA]G
Bsp1286I 393 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 330 TGTACA T^GTACA
BspDI 214 ATCGAT AT^CGAT
BspHI 423 TCATGA T^CATGA
BsrDI 741 CATTGC GCAATG(2/0)
BsrGI 330 TGTACA T^GTACA
BssKI 604 CCTGG ^CC[ACGT]GG
BssSI 5 CTCGTG CACGAG(-5/-1)
BstAPI 48 GCATTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 532 GGTAACC G^GT[ACGT]ACC
BstNI 604 CCTGG CC^[AT]GG
BstPI 532 GGTAACC G^GT[ACGT]ACC
BstXI 43 CCAAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 214 ATCGAT AT^CGAT
DsaI 700 CCATGG C^C[GA][CT]GG
EcoNI 456 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 532 GGTAACC G^GT[ACGT]ACC
EcoRII 604 CCTGG ^CC[AT]GG
FbaI 581 TGATCA T^GATCA
HaeIII 354 GGCC GG^CC
MaeI 565 CTAG C^TAG
MslI 459 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 199 CAGCGG C[AC]G^C[GT]G
MvaI 604 CCTGG CC^[AT]GG
NcoI 700 CCATGG C^CATGG
NspI 194 GCATGC [GA]CATG^[CT]
PleI 420 GACTC GAGTC(4/5)
PmaCI 625 CACGTG CAC^GTG
PmlI 625 CACGTG CAC^GTG
RcaI 423 TCATGA T^CATGA
ScrFI 604 CCTGG CC^[ACGT]GG
SmlI 154 CTTAAG C^T[CT][GA]AG
SphI 194 GCATGC GCATG^C
SspBI 330 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 331 GTAC GT^AC
360 GTAC
AgeI 70 ACCGGT A^CCGGT
97 ACCGGT
AluI 39 AGCT AG^CT
649 AGCT
AlwI 451 GATCC GGATC(4/5)
607 GGATC
AocI 141 CCTGAGG CC^T[ACGT]AGG
346 CCTGAGG
BsaWI 70 ACCGGT [AT]^CCGG[AT]
97 ACCGGT
BseRI 83 CTCCTC GAGGAG(10/8)
349 GAGGAG
BsmFI 11 GTCCC GGGAC(10/14)
101 GTCCC
BsrFI 70 ACCGGT [GA]^CCGG[CT]
97 ACCGGT
BstYI 383 AGATCT [GA]^GATC[CT]
607 GGATCT
Bsu36I 141 CCTGAGG CC^T[ACGT]AGG
346 CCTGAGG
Cac8I 194 GCATGC GC[ACGT]^[ACGT]GC
430 GCCCGC
Cfr10I 70 ACCGGT [GA]^CCGG[CT]
97 ACCGGT
DdeI 142 CTGAG C^T[ACGT]AG
347 CTGAG
EarI 710 CTCTTC CTCTTC(1/4)
748 GAAGAG
Eco81I 141 CCTGAGG CC^T[ACGT]AGG
346 CCTGAGG
FauI 431 CCCGC CCCGC(4/6)
30 GCGGG
HapII 71 CCGG C^CGG
98 CCGG
HpaII 71 CCGG C^CGG
98 CCGG
Ksp632I 710 CTCTTC CTCTTC(1/4)
748 GAAGAG
MaeII 617 ACGT A^CGT
626 ACGT
MflI 383 AGATCT [GA]^GATC[CT]
607 GGATCT
MseI 155 TTAA T^TAA
209 TTAA
MspI 71 CCGG C^CGG
98 CCGG
PinAI 70 ACCGGT A^CCGGT
97 ACCGGT
RsaI 331 GTAC GT^AC
360 GTAC
TfiI 254 GATTC G^A[AT]TC
404 GAATC
Tru9I 155 TTAA T^TAA
209 TTAA
Tsp45I 418 GTGAC ^GT[GC]AC
547 GTGAC
TspRI 528 CACTG CA[GC]TG[ACGT][ACGT]^
783 CAGTG
XhoII 383 AGATCT [GA]^GATC[CT]
607 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AvaII BbvI BsaJI BsiYI
BslI BsmAI BsrI BstF5I Cfr13I
DpnII EcoT14I Fnu4HI FokI HinfI
HphI ItaI MaeIII MboI MboII
MnlI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I SfaNI StyI TaqI
TseI Tsp509I TthHB8I