Restriction map for
pFN21AE2497
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AclI AcsI AcyI AflII
AhdI ApaI ApoI AscI AseI
AsnI AspEI AviII AvrII BamHI
BbeI BcgI BciVI BfrI BglI
BglII BlnI BsaBI BsaHI BsiEI
BsiWI BsmBI BspDI BsrBI BssHII
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoRI EcoT22I
FseI FspI HgaI Hin1I HpaI
KasI KspI MamI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RsrII
SacI SacII ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspI SwaI XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 2368 GGTACC G^GTACC
AccI 1579 GTCGAC GT^[AC][GT]AC
AccIII 1526 TCCGGA T^CCGGA
AgeI 1532 ACCGGT A^CCGGT
AocI 825 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2219 AGCGCT AGC^GCT
Asp700I 198 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2368 GGTACC G^GTACC
BalI 573 TGGCCA TGG^CCA
BclI 1718 TGATCA T^GATCA
BcnI 93 CCCGG CC^[GC]GG
BfaI 1301 CTAG C^TAG
BsaI 821 GAGACC GGTCTC(1/5)
BseAI 1526 TCCGGA T^CCGGA
Bsp1407I 917 TGTACA T^GTACA
BspEI 1526 TCCGGA T^CCGGA
BspLU11I 1355 ACATGT A^CATGT
BsrGI 917 TGTACA T^GTACA
BssSI 976 CACGAG CACGAG(-5/-1)
Bsu36I 825 CCTGAGG CC^T[ACGT]AGG
Eco47III 2219 AGCGCT AGC^GCT
Eco81I 825 CCTGAGG CC^T[ACGT]AGG
EcoRV 1270 GATATC GAT^ATC
FbaI 1718 TGATCA T^GATCA
HindIII 135 AAGCTT A^AGCTT
KpnI 2368 GGTACC GGTAC^C
MaeI 1301 CTAG C^TAG
MluNI 573 TGGCCA TGG^CCA
MroI 1526 TCCGGA T^CCGGA
MscI 573 TGGCCA TGG^CCA
MseI 2329 TTAA T^TAA
NciI 93 CCCGG CC^[GC]GG
PinAI 1532 ACCGGT A^CCGGT
PshAI 993 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1579 GTCGAC G^TCGAC
SapI 1281 GCTCTTC GCTCTTC(1/4)
SbfI 1002 CCTGCAGG CCTGCA^GG
SexAI 651 ACCAGGT A^CC[AT]GGT
SmlI 1894 CTCAAG C^T[CT][GA]AG
Sse8387I 1002 CCTGCAGG CCTGCA^GG
SspBI 917 TGTACA T^GTACA
StuI 1338 AGGCCT AGG^CCT
TaqI 1580 TCGA T^CGA
Tru9I 2329 TTAA T^TAA
Tsp509I 952 AATT ^AATT
TthHB8I 1580 TCGA T^CGA
XmnI 198 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 706 CGCG CG^CG
2256 CGCG
AflIII 413 ACACGT A^C[GA][CT]GT
1355 ACATGT
Alw44I 417 GTGCAC G^TGCAC
1690 GTGCAC
ApaLI 417 GTGCAC G^TGCAC
1690 GTGCAC
BbrPI 414 CACGTG CAC^GTG
2134 CACGTG
BsaWI 1526 TCCGGA [AT]^CCGG[AT]
1532 ACCGGT
BsmI 256 GAATGC GAATGC(1/-1)
1097 GCATTC
BspHI 1448 TCATGA T^CATGA
2381 TCATGA
BsrDI 1239 CATTGC GCAATG(2/0)
569 GCAATG
BstEII 655 GGTGACC G^GT[ACGT]ACC
930 GGTGACC
BstPI 655 GGTGACC G^GT[ACGT]ACC
930 GGTGACC
BstUI 706 CGCG CG^CG
2256 CGCG
BstYI 1412 AGATCC [GA]^GATC[CT]
2162 AGATCC
DraII 620 AGGACCT [GA]G^G[ACGT]CC[CT]
1670 GGGTCCT
DraIII 650 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1864 CACAAGGTG
EarI 1282 CTCTTC CTCTTC(1/4)
152 GAAGAG
EcoO109I 620 AGGACCT [GA]G^G[ACGT]CC[CT]
1670 GGGTCCT
EcoO65I 655 GGTGACC G^GT[ACGT]ACC
930 GGTGACC
HincII 1579 GTCGAC GT[CT]^[GA]AC
2020 GTTGAC
HindII 1579 GTCGAC GT[CT]^[GA]AC
2020 GTTGAC
Ksp632I 1282 CTCTTC CTCTTC(1/4)
152 GAAGAG
MflI 1412 AGATCC [GA]^GATC[CT]
2162 AGATCC
MvnI 706 CGCG CG^CG
2256 CGCG
PmaCI 414 CACGTG CAC^GTG
2134 CACGTG
PmlI 414 CACGTG CAC^GTG
2134 CACGTG
PpuMI 620 AGGACCT [GA]G^G[AT]CC[CT]
1670 GGGTCCT
RcaI 1448 TCATGA T^CATGA
2381 TCATGA
SphI 274 GCATGC GCATG^C
2315 GCATGC
XhoII 1412 AGATCC [GA]^GATC[CT]
2162 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AspI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstXI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NaeI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
TseI Tsp45I TspRI Tth111I Van91I
XcmI