Restriction map for
pFN21AE5261
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# NO CUT ENZYMES ( 193 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FokI FseI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MslI MspI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AlwI 255 GGATC GGATC(4/5)
AlwNI 430 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 397 GTGCTC G[AT]GC[AT]^C
BbsI 457 GTCTTC GAAGAC(2/6)
BciVI 143 GTATCC GTATCC(6/5)
BfaI 334 CTAG C^TAG
BpuAI 457 GTCTTC GAAGAC(2/6)
BsaJI 147 CCAAGG C^C[ACGT][ACGT]GG
BsiHKAI 397 GTGCTC G[AT]GC[AT]^C
BsrI 207 CCAGT ACTGG(1/-1)
BssKI 245 CCTGG ^CC[ACGT]GG
BstAPI 522 GCACAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 245 CCTGG CC^[AT]GG
BstXI 476 CCATCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 256 GATC ^GATC
EarI 136 GAAGAG CTCTTC(1/4)
EcoRII 245 CCTGG ^CC[AT]GG
EcoT14I 147 CCAAGG C^C[AT][AT]GG
HphI 323 TCACC GGTGA(8/7)
Ksp632I 136 GAAGAG CTCTTC(1/4)
MaeI 334 CTAG C^TAG
MaeII 338 ACGT A^CGT
MboI 256 GATC ^GATC
MspA1I 314 CCGCTG C[AC]G^C[GT]G
MvaI 245 CCTGG CC^[AT]GG
NdeI 7 CATATG CA^TATG
NdeII 256 GATC ^GATC
NlaIV 373 GGCTCC GG[ACGT]^[ACGT]CC
PstI 317 CTGCAG CTGCA^G
Sau3AI 256 GATC ^GATC
ScrFI 245 CCTGG CC^[ACGT]GG
SfcI 317 CTGCAG C^T[GA][CT]AG
StyI 147 CCAAGG C^C[AT][AT]GG
Tsp509I 354 AATT ^AATT
TspRI 381 CACTG CA[GC]TG[ACGT][ACGT]^
XbaI 333 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 314 CCGC CCGC(-3/-1)
423 GCGG
AluI 294 AGCT AG^CT
526 AGCT
AvaII 204 GGTCC G^G[AT]CC
242 GGTCC
BmyI 80 GGGCAC G[AGT]GC[ACT]^C
397 GTGCTC
BsiYI 408 CCTGCATGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
462 CCCTCGAATGG
BslI 408 CCTGCATGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
462 CCCTCGAATGG
Bsp1286I 80 GGGCAC G[AGT]GC[ACT]^C
397 GTGCTC
BspMI 301 GCAGGT ACCTGC(4/8)
451 GCAGGT
Cfr13I 204 GGTCC G^G[ACGT]CC
242 GGTCC
MaeIII 322 GTCAC ^GT[ACGT]AC
426 GTCAC
MboII 136 GAAGA GAAGA(8/7)
458 TCTTC
NlaIII 259 CATG CATG^
412 CATG
PleI 223 GAGTC GAGTC(4/5)
552 GAGTC
Sau96I 204 GGTCC G^G[ACGT]CC
242 GGTCC
Tsp45I 322 GTCAC ^GT[GC]AC
426 GTCAC
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
BbvI BpmI Cac8I Fnu4HI HinfI
ItaI MnlI MseI MwoI TaqI
TfiI Tru9I TseI TthHB8I