Restriction map for
pFN21AE5436
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstYI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tsp509I Tth111I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 855 TCCGGA T^CCGGA
AcyI 658 GACGCC G[GA]^CG[CT]C
AfaI 954 GTAC GT^AC
AlwI 702 GATCC GGATC(4/5)
AocI 392 CCTCAGG CC^T[ACGT]AGG
BalI 344 TGGCCA TGG^CCA
BbsI 709 GTCTTC GAAGAC(2/6)
BbvCI 264 CCTCAGC CCTCAGC(-5/-2)
BfaI 25 CTAG C^TAG
BpuAI 709 GTCTTC GAAGAC(2/6)
BsaHI 658 GACGCC G[GA]^CG[CT]C
BsaI 889 GAGACC GGTCTC(1/5)
BsaWI 855 TCCGGA [AT]^CCGG[AT]
BseAI 855 TCCGGA T^CCGGA
BseRI 621 CTCCTC GAGGAG(10/8)
BsgI 970 GTGCAG GTGCAG(16/14)
BsmFI 175 GGGAC GGGAC(10/14)
BsmI 153 GCATTC GAATGC(1/-1)
BspEI 855 TCCGGA T^CCGGA
BspHI 485 TCATGA T^CATGA
BsrBI 595 CCGCTC CCGCTC(-3/-3)
BsrDI 768 CATTGC GCAATG(2/0)
BstEII 162 GGTCACC G^GT[ACGT]ACC
BstPI 162 GGTCACC G^GT[ACGT]ACC
Bsu36I 392 CCTCAGG CC^T[ACGT]AGG
DpnII 702 GATC ^GATC
DsaI 794 CCGTGG C^C[GA][CT]GG
Eco81I 392 CCTCAGG CC^T[ACGT]AGG
EcoNI 186 CCTCATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 162 GGTCACC G^GT[ACGT]ACC
FseI 587 GGCCGGCC GGCCGG^CC
Hin1I 658 GACGCC G[GA]^CG[CT]C
HinfI 482 GAATC G^A[ACGT]TC
MaeI 25 CTAG C^TAG
MaeIII 163 GTCAC ^GT[ACGT]AC
MboI 702 GATC ^GATC
MluNI 344 TGGCCA TGG^CCA
MroI 855 TCCGGA T^CCGGA
MscI 344 TGGCCA TGG^CCA
MseI 65 TTAA T^TAA
MslI 963 CATGACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 702 GATC ^GATC
PflMI 213 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 313 CAGCTG CAG^CTG
RcaI 485 TCATGA T^CATGA
RsaI 954 GTAC GT^AC
SapI 597 GCTCTTC GCTCTTC(1/4)
Sau3AI 702 GATC ^GATC
SfaNI 366 GCATC GCATC(5/9)
TaqI 905 TCGA T^CGA
TfiI 482 GAATC G^A[AT]TC
Tru9I 65 TTAA T^TAA
Tsp45I 163 GTCAC ^GT[GC]AC
TthHB8I 905 TCGA T^CGA
Van91I 213 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
ApaI 182 GGGCCC GGGCC^C
409 GGGCCC
BglI 269 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
583 GCCTGGCCGGC
BpmI 529 CTGGAG CTGGAG(16/14)
640 CTGGAG
BsaAI 475 TACGTG [CT]AC^GT[GA]
736 TACGTG
BsmAI 451 GAGAC GTCTC(1/5)
889 GAGAC
BspMI 362 ACCTGC ACCTGC(4/8)
1008 ACCTGC
EcoT14I 780 CCTTGG C^C[AT][AT]GG
872 CCTTGG
HgaI 324 GACGC GACGC(5/10)
658 GACGC
NaeI 588 GCCGGC GCC^GGC
775 GCCGGC
NgoMIV 588 GCCGGC G^CCGGC
775 GCCGGC
NspI 725 GCATGC [GA]CATG^[CT]
947 GCATGC
PpuMI 175 GGGACCT [GA]G^G[AT]CC[CT]
359 AGGACCT
SfcI 462 CTACAG C^T[GA][CT]AG
912 CTACAG
SmaI 544 CCCGGG CCC^GGG
809 CCCGGG
SphI 725 GCATGC GCATG^C
947 GCATGC
StyI 780 CCTTGG C^C[AT][AT]GG
872 CCTTGG
XmaCI 544 CCCGGG C^CCGGG
809 CCCGGG
XmaI 544 CCCGGG C^CCGGG
809 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaI
AvaII BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
TseI TspRI