Restriction map for
pFN21AB5762
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI ApoI
AscI AseI AsnI AspEI AviII
AvrII BbeI BbrPI BbsI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
CelII ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SalI SapI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SspBI SspI SwaI TfiI XbaI
XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AocI 2191 CCTGAGG CC^T[ACGT]AGG
Asp700I 1578 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1222 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2186 GGATCC G^GATCC
BcgI 1240 CGAGCCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1606 CTAG C^TAG
BglI 1275 GCCCCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1177 GAGACC GGTCTC(1/5)
BspHI 1146 TCATGA T^CATGA
BsrBI 891 GAGCGG CCGCTC(-3/-3)
BsrDI 1035 CATTGC GCAATG(2/0)
BsrFI 3 GCCGGC [GA]^CCGG[CT]
BssSI 658 CTCGTG CACGAG(-5/-1)
BstEII 310 GGTGACC G^GT[ACGT]ACC
BstPI 310 GGTGACC G^GT[ACGT]ACC
Bsu36I 2191 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
DraI 1303 TTTAAA TTT^AAA
DrdI 775 GACGTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 841 CTCTTC CTCTTC(1/4)
Eco81I 2191 CCTGAGG CC^T[ACGT]AGG
EcoNI 1847 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 310 GGTGACC G^GT[ACGT]ACC
EcoT22I 1435 ATGCAT ATGCA^T
Ksp632I 841 CTCTTC CTCTTC(1/4)
KspI 861 CCGCGG CCGC^GG
MaeI 1606 CTAG C^TAG
NaeI 3 GCCGGC GCC^GGC
NgoMIV 3 GCCGGC G^CCGGC
NsiI 1435 ATGCAT ATGCA^T
PflFI 1222 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1146 TCATGA T^CATGA
SacI 652 GAGCTC GAGCT^C
SacII 861 CCGCGG CCGC^GG
ScaI 1673 AGTACT AGT^ACT
StuI 391 AGGCCT AGG^CCT
Tth111I 1222 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1412 CCAACGGGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1578 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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Acc65I 398 GGTACC G^GTACC
1856 GGTACC
AccI 1372 GTCTAC GT^[AC][GT]AC
1552 GTAGAC
Aor51HI 611 AGCGCT AGC^GCT
2180 AGCGCT
ApaI 349 GGGCCC GGGCC^C
361 GGGCCC
Asp718I 398 GGTACC G^GTACC
1856 GGTACC
BalI 191 TGGCCA TGG^CCA
582 TGGCCA
BbvCI 159 GCTGAGG CCTCAGC(-5/-2)
246 GCTGAGG
BstYI 1787 AGATCC [GA]^GATC[CT]
2186 GGATCC
DraIII 1534 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1639 CACCGCGTG
Eco47III 611 AGCGCT AGC^GCT
2180 AGCGCT
HgaI 1668 GACGC GACGC(5/10)
1139 GCGTC
HinfI 72 GACTC G^A[ACGT]TC
1885 GAGTC
KpnI 398 GGTACC GGTAC^C
1856 GGTACC
MflI 1787 AGATCC [GA]^GATC[CT]
2186 GGATCC
MluNI 191 TGGCCA TGG^CCA
582 TGGCCA
MscI 191 TGGCCA TGG^CCA
582 TGGCCA
MseI 1292 TTAA T^TAA
1304 TTAA
MspA1I 796 CCGCTG C[AC]G^C[GT]G
861 CCGCGG
PleI 72 GACTC GAGTC(4/5)
1885 GAGTC
SbfI 462 CCTGCAGG CCTGCA^GG
1090 CCTGCAGG
SexAI 1805 ACCAGGT A^CC[AT]GGT
1991 ACCTGGT
SmaI 66 CCCGGG CCC^GGG
566 CCCGGG
SphI 1097 GCATGC GCATG^C
1437 GCATGC
Sse8387I 462 CCTGCAGG CCTGCA^GG
1090 CCTGCAGG
Tru9I 1292 TTAA T^TAA
1304 TTAA
Tsp509I 1294 AATT ^AATT
1378 AATT
XhoII 1787 AGATCC [GA]^GATC[CT]
2186 GGATCC
XmaCI 66 CCCGGG C^CCGGG
566 CCCGGG
XmaI 66 CCCGGG C^CCGGG
566 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI AlwNI ApaLI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I