Restriction map for
pFN21AE6374
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BanI BbeI BbrPI BbsI BbvCI
BciVI BfrI BglI BglII BlnI
BpuAI BsaAI BsaHI BseAI BsgI
BsiEI BsiWI BspDI BspEI BspHI
BspLU11I BsrBI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MaeII MfeI MroI
MunI NarI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 635 GTAGAC GT^[AC][GT]AC
AcsI 772 AAATTT [GA]^AATT[CT]
AflIII 424 ACGCGT A^C[GA][CT]GT
AocI 406 CCTAAGG CC^T[ACGT]AGG
ApaI 117 GGGCCC GGGCC^C
ApoI 772 AAATTT [GA]^AATT[CT]
Asp700I 319 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 843 CTCGGG C^[CT]CG[GA]G
BalI 306 TGGCCA TGG^CCA
BamHI 1068 GGATCC G^GATCC
BcgI 592 GCAACCAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 666 TGATCA T^GATCA
BpmI 694 CTCCAG CTGGAG(16/14)
BsaBI 1224 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1283 GGTCTC GGTCTC(1/5)
BsaWI 1287 TCCGGT [AT]^CCGG[AT]
BsmBI 907 GAGACG CGTCTC(1/5)
BsoBI 843 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1211 TGTACA T^GTACA
BsrDI 273 GCAATG GCAATG(2/0)
BsrFI 554 GCCGGC [GA]^CCGG[CT]
BsrGI 1211 TGTACA T^GTACA
BssSI 1119 CTCGTG CACGAG(-5/-1)
BstYI 1068 GGATCC [GA]^GATC[CT]
Bsu36I 406 CCTAAGG CC^T[ACGT]AGG
Cfr10I 554 GCCGGC [GA]^CCGG[CT]
DrdI 856 GACAGTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 306 TGGCCA [CT]^GGCC[GA]
EarI 1195 GAAGAG CTCTTC(1/4)
Eco81I 406 CCTAAGG CC^T[ACGT]AGG
EcoT22I 1206 ATGCAT ATGCA^T
FauI 146 GCGGG CCCGC(4/6)
FbaI 666 TGATCA T^GATCA
HaeII 173 GGCGCT [GA]GCGC^[CT]
HindIII 1048 AAGCTT A^AGCTT
Ksp632I 1195 GAAGAG CTCTTC(1/4)
MamI 1224 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1068 GGATCC [GA]^GATC[CT]
MluI 424 ACGCGT A^CGCGT
MluNI 306 TGGCCA TGG^CCA
MscI 306 TGGCCA TGG^CCA
MseI 655 TTAA T^TAA
NaeI 554 GCCGGC GCC^GGC
NgoMIV 554 GCCGGC G^CCGGC
NsiI 1206 ATGCAT ATGCA^T
PflMI 1094 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 660 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 266 GAGCTC GAGCT^C
SmlI 361 CTCAAG C^T[CT][GA]AG
SspBI 1211 TGTACA T^GTACA
Tru9I 655 TTAA T^TAA
Van91I 1094 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 548 CCACCTGCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1068 GGATCC [GA]^GATC[CT]
XmnI 319 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 1069 GATCC GGATC(4/5)
1068 GGATC
AspHI 266 GAGCTC G[AT]GC[AT]^C
1110 GTGCTC
BcnI 93 CCGGG CC^[GC]GG
1143 CCGGG
BfaI 400 CTAG C^TAG
755 CTAG
BsiHKAI 266 GAGCTC G[AT]GC[AT]^C
1110 GTGCTC
BsmFI 95 GGGAC GGGAC(10/14)
700 GGGAC
BsmI 4 GAATGC GAATGC(1/-1)
130 GAATGC
BsrI 309 CCAGT ACTGG(1/-1)
867 CCAGT
BstXI 471 CCAAATTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1078 CCAGCCAGGTGG
CfoI 174 GCGC GCG^C
1234 GCGC
HhaI 174 GCGC GCG^C
1234 GCGC
HinP1I 174 GCGC G^CGC
1234 GCGC
HinfI 439 GAATC G^A[ACGT]TC
834 GATTC
MaeI 400 CTAG C^TAG
755 CTAG
NciI 93 CCGGG CC^[GC]GG
1143 CCGGG
PvuII 493 CAGCTG CAG^CTG
952 CAGCTG
TfiI 439 GAATC G^A[AT]TC
834 GATTC
Tsp45I 1013 GTCAC ^GT[GC]AC
1183 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanII BbvI BlpI BmyI Bpu1102I
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BspMI BssKI BstF5I BstNI
BstUI Cac8I CelII Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NcoI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp509I TspRI