Restriction map for
pFN21AE5156
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# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AluI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HinfI
HpaI HphI KasI KpnI Ksp632I
KspI MaeI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MunI NarI NciI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tsp45I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 124 CGCG CG^CG
AspHI 299 GTGCTC G[AT]GC[AT]^C
BamHI 236 GGATCC G^GATCC
BanI 51 GGCACC G^G[CT][GA]CC
BanII 136 GAGCCC G[GA]GC[CT]^C
BbvCI 211 GCTGAGG CCTCAGC(-5/-2)
BseRI 205 GAGGAG GAGGAG(10/8)
BsgI 127 GTGCAG GTGCAG(16/14)
BsiHKAI 299 GTGCTC G[AT]GC[AT]^C
BsmFI 11 GTCCC GGGAC(10/14)
BspHI 113 TCATGA T^CATGA
BsrFI 227 GCCGGC [GA]^CCGG[CT]
BstF5I 120 CATCC GGATG(2/0)
BstUI 124 CGCG CG^CG
BstXI 140 CCAAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 236 GGATCC [GA]^GATC[CT]
Cfr10I 227 GCCGGC [GA]^CCGG[CT]
DpnII 237 GATC ^GATC
EcoT14I 47 CCAAGG C^C[AT][AT]GG
FauI 317 CCCGC CCCGC(4/6)
FokI 120 CATCC GGATG(9/13)
HaeII 194 GGCGCT [GA]GCGC^[CT]
HapII 228 CCGG C^CGG
HpaII 228 CCGG C^CGG
MboI 237 GATC ^GATC
MboII 31 TCTTC GAAGA(8/7)
MflI 236 GGATCC [GA]^GATC[CT]
MslI 120 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 190 CAGCGG C[AC]G^C[GT]G
MspI 228 CCGG C^CGG
MvnI 124 CGCG CG^CG
NaeI 227 GCCGGC GCC^GGC
NdeII 237 GATC ^GATC
NgoMIV 227 GCCGGC G^CCGGC
NlaIII 114 CATG CATG^
PstI 245 CTGCAG CTGCA^G
RcaI 113 TCATGA T^CATGA
Sau3AI 237 GATC ^GATC
SfcI 245 CTGCAG C^T[GA][CT]AG
StyI 47 CCAAGG C^C[AT][AT]GG
XhoII 236 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 237 GATCC GGATC(4/5)
236 GGATC
AvaII 81 GGACC G^G[AT]CC
327 GGTCC
BbvI 231 GCTGC GCAGC(8/12)
244 GCTGC
BmyI 136 GAGCCC G[AGT]GC[ACT]^C
299 GTGCTC
BsaI 69 GGTCTC GGTCTC(1/5)
150 GGTCTC
BsaJI 47 CCAAGG C^C[ACGT][ACGT]GG
330 CCGAGG
BsiYI 123 CCGCGTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
318 CCGCTTGGAGG
BslI 123 CCGCGTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
318 CCGCTTGGAGG
BsmAI 70 GTCTC GTCTC(1/5)
151 GTCTC
Bsp1286I 136 GAGCCC G[AGT]GC[ACT]^C
299 GTGCTC
BssKI 155 CCTGG ^CC[ACGT]GG
240 CCTGG
BstNI 155 CCTGG CC^[AT]GG
240 CCTGG
CfoI 180 GCGC GCG^C
195 GCGC
DdeI 160 CTGAG C^T[ACGT]AG
212 CTGAG
EcoRII 155 CCTGG ^CC[AT]GG
240 CCTGG
HaeIII 100 GGCC GG^CC
315 GGCC
HhaI 180 GCGC GCG^C
195 GCGC
HinP1I 180 GCGC G^CGC
195 GCGC
MvaI 155 CCTGG CC^[AT]GG
240 CCTGG
ScrFI 155 CCTGG CC^[ACGT]GG
240 CCTGG
SfaNI 259 GATGC GCATC(5/9)
119 GCATC
TseI 231 GCTGC G^C[AT]GC
244 GCTGC
TspRI 39 CACTG CA[GC]TG[ACGT][ACGT]^
307 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
AciI BsrI Cac8I Cfr13I Fnu4HI
ItaI MnlI MwoI NlaIV Sau96I