##################################
# NO CUT ENZYMES (  214 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanII       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaBI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FokI        FseI        FspI        HaeIII      
HapII       HincII      HindII      HindIII     HinfI       
HpaI        HpaII       ItaI        KpnI        Ksp632I     
KspI        MaeIII      MamI        MboI        MboII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MspA1I      MspI        MunI        
MvaI        MvnI        NaeI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        ScrFI       SexAI       SfcI        
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AcyI           44  GGCGCC               G[GA]^CG[CT]C
AfaI          123  GTAC                 GT^AC
AluI          211  AGCT                 AG^CT
AvaII         144  GGACC                G^G[AT]CC
BanI           44  GGCGCC               G^G[CT][GA]CC
BbeI           44  GGCGCC               GGCGC^C
BbrPI         148  CACGTG               CAC^GTG
BfaI           14  CTAG                 C^TAG
BsaAI         148  CACGTG               [CT]AC^GT[GA]
BsaHI          44  GGCGCC               G[GA]^CG[CT]C
Bsp1407I      122  TGTACA               T^GTACA
BsrGI         122  TGTACA               T^GTACA
BsrI          169  ACTGG                ACTGG(1/-1)
Cac8I          47  GCCTGC               GC[ACGT]^[ACGT]GC
Cfr13I        144  GGACC                G^G[ACGT]CC
DdeI           52  CTTAG                C^T[ACGT]AG
HaeII          44  GGCGCC               [GA]GCGC^[CT]
HgaI           60  GACGC                GACGC(5/10)
Hin1I          44  GGCGCC               G[GA]^CG[CT]C
KasI           44  GGCGCC               G^GCGCC
MaeI           14  CTAG                 C^TAG
MslI          114  CACTGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI          104  GCTCGGTGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI           44  GGCGCC               GG^CGCC
NlaIII        174  CATG                 CATG^
NlaIV          44  GGCGCC               GG[ACGT]^[ACGT]CC
PmaCI         148  CACGTG               CAC^GTG
PmlI          148  CACGTG               CAC^GTG
RsaI          123  GTAC                 GT^AC
Sau96I        144  GGACC                G^G[ACGT]CC
SfoI           44  GGCGCC               GGC^GCC
SspBI         122  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AspHI          37  GAGCAC               G[AT]GC[AT]^C
              111  GAGCAC              
BmyI           37  GAGCAC               G[AGT]GC[ACT]^C
              111  GAGCAC              
BsiHKAI        37  GAGCAC               G[AT]GC[AT]^C
              111  GAGCAC              
Bsp1286I       37  GAGCAC               G[AGT]GC[ACT]^C
              111  GAGCAC              
CfoI           45  GCGC                 GCG^C
               69  GCGC                
HhaI           45  GCGC                 GCG^C
               69  GCGC                
HinP1I         45  GCGC                 G^CGC
               69  GCGC                
HphI          108  GGTGA                GGTGA(8/7)
               83  TCACC               
MaeII          74  ACGT                 A^CGT
              149  ACGT                
MnlI            6  CCTC                 CCTC(7/6)
               86  CCTC                
SfaNI          66  GATGC                GCATC(5/9)
              204  GATGC               
TspRI          18  CAGTG                CA[GC]TG[ACGT][ACGT]^
              114  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################