Restriction map for
pFN21AE5663
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# NO CUT ENZYMES ( 119 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AvaI AviII AvrII
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BlnI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BspLU11I BspMI BsrFI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HphI KasI KpnI KspI MamI
MluI MroI MslI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SwaI Tsp45I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 44 GACGTC GACGT^C
AfaI 1385 GTAC GT^AC
AflIII 1136 ACACGT A^C[GA][CT]GT
AseI 976 ATTAAT AT^TAAT
AsnI 976 ATTAAT AT^TAAT
AspHI 406 GAGCTC G[AT]GC[AT]^C
AspI 1279 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 752 TGGCCA TGG^CCA
BamHI 426 GGATCC G^GATCC
BbvCI 1093 GCTGAGG CCTCAGC(-5/-2)
BcnI 1036 CCGGG CC^[GC]GG
BglII 1369 AGATCT A^GATCT
BlpI 938 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 938 GCTCAGC GC^T[ACGT]AGC
BsaI 472 GGTCTC GGTCTC(1/5)
BseRI 920 GAGGAG GAGGAG(10/8)
BsiHKAI 406 GAGCTC G[AT]GC[AT]^C
BsmFI 1277 GGGAC GGGAC(10/14)
BsmI 1396 GCATTC GAATGC(1/-1)
Bsp1407I 1384 TGTACA T^GTACA
BspHI 902 TCATGA T^CATGA
BsrBI 461 GAGCGG CCGCTC(-3/-3)
BsrDI 730 GCAATG GCAATG(2/0)
BsrGI 1384 TGTACA T^GTACA
BstBI 1303 TTCGAA TT^CGAA
CelII 938 GCTCAGC GC^T[ACGT]AGC
CfoI 1120 GCGC GCG^C
DraII 101 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 1184 CCATGG C^C[GA][CT]GG
EcoO109I 101 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1408 GAATTC G^AATTC
EcoT14I 1184 CCATGG C^C[AT][AT]GG
HhaI 1120 GCGC GCG^C
HinP1I 1120 GCGC G^CGC
HincII 1348 GTTAAC GT[CT]^[GA]AC
HindII 1348 GTTAAC GT[CT]^[GA]AC
HindIII 961 AAGCTT A^AGCTT
HpaI 1348 GTTAAC GTT^AAC
MfeI 601 CAATTG C^AATTG
MluNI 752 TGGCCA TGG^CCA
MscI 752 TGGCCA TGG^CCA
MunI 601 CAATTG C^AATTG
NciI 1036 CCGGG CC^[GC]GG
NcoI 1184 CCATGG C^CATGG
NspI 1381 GCATGT [GA]CATG^[CT]
NspV 1303 TTCGAA TT^CGAA
PflFI 1279 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 704 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 976 ATTAAT AT^TAAT
RcaI 902 TCATGA T^CATGA
RsaI 1385 GTAC GT^AC
SacI 406 GAGCTC GAGCT^C
SfuI 1303 TTCGAA TT^CGAA
SspBI 1384 TGTACA T^GTACA
SspI 979 AATATT AAT^ATT
StuI 551 AGGCCT AGG^CCT
StyI 1184 CCATGG C^C[AT][AT]GG
Tth111I 1279 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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Asp700I 116 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1404 GAAAGAATTC
BanI 1180 GGTGCC G^G[CT][GA]CC
1207 GGTGCC
BanII 406 GAGCTC G[GA]GC[CT]^C
769 GAGCCC
BfaI 292 CTAG C^TAG
677 CTAG
BpmI 403 CTGGAG CTGGAG(16/14)
1287 CTGGAG
BsgI 161 GTGCAG GTGCAG(16/14)
384 GTGCAG
EaeI 50 TGGCCG [CT]^GGCC[GA]
752 TGGCCA
FauI 25 GCGGG CCCGC(4/6)
379 GCGGG
MaeI 292 CTAG C^TAG
677 CTAG
MaeIII 1127 GTTAC ^GT[ACGT]AC
1414 GTAAC
PflMI 600 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
707 CCAAGTCTTGG
PstI 1373 CTGCAG CTGCA^G
1391 CTGCAG
PvuII 314 CAGCTG CAG^CTG
1388 CAGCTG
SfcI 1373 CTGCAG C^T[GA][CT]AG
1391 CTGCAG
Van91I 600 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
707 CCAAGTCTTGG
XmnI 116 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1404 GAAAGAATTC
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
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AciI AcsI AcyI AluI AlwI
ApoI AvaII BbsI BbvI BmyI
BpuAI BsaHI BsaJI BsiYI BslI
BsmAI BsmBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HinfI HpaII ItaI Ksp632I MaeII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII