Restriction map for
pFN21AE6653
##################################
# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BlnI BpuAI
BsaAI BsaBI BsaHI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MscI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tru9I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccII 190 CGCG CG^CG
AcsI 626 GAATTC [GA]^AATT[CT]
AlwNI 640 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 626 GAATTC [GA]^AATT[CT]
BamHI 388 GGATCC G^GATCC
BcnI 422 CCCGG CC^[GC]GG
BglII 43 AGATCT A^GATCT
BlpI 260 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 260 GCTCAGC GC^T[ACGT]AGC
BsgI 138 GTGCAG GTGCAG(16/14)
BstUI 190 CGCG CG^CG
BstXI 323 CCAAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 256 GCTCGC GC[ACGT]^[ACGT]GC
CelII 260 GCTCAGC GC^T[ACGT]AGC
DraIII 321 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 626 GAATTC G^AATTC
EcoT14I 708 CCAAGG C^C[AT][AT]GG
HincII 175 GTCAAC GT[CT]^[GA]AC
HindII 175 GTCAAC GT[CT]^[GA]AC
MaeIII 602 GTGAC ^GT[ACGT]AC
MspA1I 64 CAGCTG C[AC]G^C[GT]G
MvnI 190 CGCG CG^CG
NciI 422 CCCGG CC^[GC]GG
NspI 507 ACATGC [GA]CATG^[CT]
PvuII 64 CAGCTG CAG^CTG
SapI 414 GAAGAGC GCTCTTC(1/4)
SmlI 721 CTCAAG C^T[CT][GA]AG
StyI 708 CCAAGG C^C[AT][AT]GG
TfiI 296 GAATC G^A[AT]TC
Tsp45I 602 GTGAC ^GT[GC]AC
Tsp509I 627 AATT ^AATT
XcmI 323 CCAAGTGGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 494 TCCGGA T^CCGGA
548 TCCGGA
AlwI 389 GATCC GGATC(4/5)
388 GGATC
ApaI 122 GGGCCC GGGCC^C
196 GGGCCC
AspHI 371 GAGCAC G[AT]GC[AT]^C
417 GAGCAC
AvaII 26 GGACC G^G[AT]CC
131 GGTCC
BanI 280 GGTGCC G^G[CT][GA]CC
319 GGCACC
BfaI 607 CTAG C^TAG
658 CTAG
BglI 311 GCCAAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
667 GCCATTATGGC
BsaI 109 GAGACC GGTCTC(1/5)
337 GAGACC
BsaWI 494 TCCGGA [AT]^CCGG[AT]
548 TCCGGA
BseAI 494 TCCGGA T^CCGGA
548 TCCGGA
BseRI 561 CTCCTC GAGGAG(10/8)
368 GAGGAG
BsiHKAI 371 GAGCAC G[AT]GC[AT]^C
417 GAGCAC
BsmAI 109 GAGAC GTCTC(1/5)
337 GAGAC
BspEI 494 TCCGGA T^CCGGA
548 TCCGGA
BstF5I 497 GGATG GGATG(2/0)
726 GGATG
BstYI 43 AGATCT [GA]^GATC[CT]
388 GGATCC
CfoI 36 GCGC GCG^C
191 GCGC
FauI 534 GCGGG CCCGC(4/6)
622 GCGGG
FokI 497 GGATG GGATG(9/13)
726 GGATG
HhaI 36 GCGC GCG^C
191 GCGC
HinP1I 36 GCGC G^CGC
191 GCGC
HphI 345 GGTGA GGTGA(8/7)
480 TCACC
MaeI 607 CTAG C^TAG
658 CTAG
MflI 43 AGATCT [GA]^GATC[CT]
388 GGATCC
MroI 494 TCCGGA T^CCGGA
548 TCCGGA
NlaIII 21 CATG CATG^
508 CATG
PleI 304 GAGTC GAGTC(4/5)
592 GAGTC
PpuMI 25 GGGACCT [GA]G^G[AT]CC[CT]
130 GGGTCCC
PstI 85 CTGCAG CTGCA^G
251 CTGCAG
SfaNI 477 GCATC GCATC(5/9)
633 GCATC
TspRI 384 CAGTG CA[GC]TG[ACGT][ACGT]^
600 CAGTG
XhoII 43 AGATCT [GA]^GATC[CT]
388 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
ItaI Ksp632I MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI SfcI TseI