Restriction map for
pFN21AE6475
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AvaI AviII BamHI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BsaBI BsgI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspHI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MflI
MluI MunI MvnI NciI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PleI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SpeI SphI Sse8387I SspBI
SwaI XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccIII 990 TCCGGA T^CCGGA
AcsI 223 AAATTC [GA]^AATT[CT]
AgeI 1216 ACCGGT A^CCGGT
AlwI 3 GGATC GGATC(4/5)
ApoI 223 AAATTC [GA]^AATT[CT]
AseI 1254 ATTAAT AT^TAAT
AsnI 1254 ATTAAT AT^TAAT
Asp700I 716 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1120 GAGCTC G[AT]GC[AT]^C
AspI 247 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 861 GGCGCC G^G[CT][GA]CC
BanII 1120 GAGCTC G[GA]GC[CT]^C
BbeI 861 GGCGCC GGCGC^C
BlpI 293 GCTTAGC GC^T[ACGT]AGC
BmyI 1120 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 293 GCTTAGC GC^T[ACGT]AGC
BsaAI 1018 TACGTA [CT]AC^GT[GA]
BsaI 252 GGTCTC GGTCTC(1/5)
BseAI 990 TCCGGA T^CCGGA
BseRI 479 GAGGAG GAGGAG(10/8)
BsiEI 887 CGGTCG CG[GA][CT]^CG
BsiHKAI 1120 GAGCTC G[AT]GC[AT]^C
BsmFI 1134 GGGAC GGGAC(10/14)
BsmI 145 GCATTC GAATGC(1/-1)
Bsp1286I 1120 GAGCTC G[AGT]GC[ACT]^C
BspEI 990 TCCGGA T^CCGGA
BspLU11I 151 ACATGT A^CATGT
BspMI 1237 GCAGGT ACCTGC(4/8)
BstXI 1061 CCATCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 293 GCTTAGC GC^T[ACGT]AGC
CfoI 862 GCGC GCG^C
DraI 844 TTTAAA TTT^AAA
DraII 402 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1009 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1200 CCTTGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 402 AGGTCCT [GA]G^G[ACGT]CC[CT]
HaeII 861 GGCGCC [GA]GCGC^[CT]
HgaI 788 GCGTC GACGC(5/10)
HhaI 862 GCGC GCG^C
HinP1I 862 GCGC G^CGC
HincII 1105 GTTGAC GT[CT]^[GA]AC
HindII 1105 GTTGAC GT[CT]^[GA]AC
KasI 861 GGCGCC G^GCGCC
MroI 990 TCCGGA T^CCGGA
NaeI 858 GCCGGC GCC^GGC
NarI 861 GGCGCC GG^CGCC
NcoI 1067 CCATGG C^CATGG
NgoMIV 858 GCCGGC G^CCGGC
PflFI 247 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 325 CCAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1216 ACCGGT A^CCGGT
PpuMI 402 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1254 ATTAAT AT^TAAT
SacI 1120 GAGCTC GAGCT^C
SexAI 1100 ACCAGGT A^CC[AT]GGT
SfcI 585 CTGTAG C^T[GA][CT]AG
SfoI 861 GGCGCC GGC^GCC
SnaBI 1018 TACGTA TAC^GTA
SspI 327 AATATT AAT^ATT
StuI 58 AGGCCT AGG^CCT
Tth111I 247 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 325 CCAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 716 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 787 GGCGTC G[GA]^CG[CT]C
861 GGCGCC
AflIII 151 ACATGT A^C[GA][CT]GT
773 ACGTGT
AlwNI 647 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
740 CAGTTGCTG
AvrII 576 CCTAGG C^CTAGG
783 CCTAGG
BalI 602 TGGCCA TGG^CCA
1070 TGGCCA
BbsI 1224 GAAGAC GAAGAC(2/6)
661 GTCTTC
BclI 1367 TGATCA T^GATCA
1379 TGATCA
BlnI 576 CCTAGG C^CTAGG
783 CCTAGG
BpuAI 1224 GAAGAC GAAGAC(2/6)
661 GTCTTC
BsaHI 787 GGCGTC G[GA]^CG[CT]C
861 GGCGCC
BsmAI 253 GTCTC GTCTC(1/5)
1191 GTCTC
BsrFI 858 GCCGGC [GA]^CCGG[CT]
1216 ACCGGT
BsrI 901 ACTGG ACTGG(1/-1)
1282 ACTGG
BstF5I 73 CATCC GGATG(2/0)
945 GGATG
Cfr10I 858 GCCGGC [GA]^CCGG[CT]
1216 ACCGGT
EaeI 602 TGGCCA [CT]^GGCC[GA]
1070 TGGCCA
FbaI 1367 TGATCA T^GATCA
1379 TGATCA
FokI 73 CATCC GGATG(9/13)
945 GGATG
Hin1I 787 GGCGTC G[GA]^CG[CT]C
861 GGCGCC
HphI 244 GGTGA GGTGA(8/7)
665 TCACC
MaeII 773 ACGT A^CGT
1019 ACGT
MluNI 602 TGGCCA TGG^CCA
1070 TGGCCA
MscI 602 TGGCCA TGG^CCA
1070 TGGCCA
MwoI 304 GCAATGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
508 GCCAGAGATGC
NspI 151 ACATGT [GA]CATG^[CT]
657 GCATGT
SfaNI 514 GATGC GCATC(5/9)
1024 GATGC
SmlI 287 CTCAAG C^T[CT][GA]AG
519 CTTGAG
TaqI 447 TCGA T^CGA
1194 TCGA
TthHB8I 447 TCGA T^CGA
1194 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BfaI BpmI BsaJI BsaWI BsiYI
BslI BsrDI BssKI BstNI Cac8I
Cfr13I DdeI DpnII DsaI EarI
EcoRII EcoT14I Fnu4HI HaeIII HapII
HinfI HpaII ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI