Restriction map for
pFN21AE5346
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BlpI BmyI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoT22I FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaII KasI KpnI KspI
MaeII MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 39 GTCTAC GT^[AC][GT]AC
AflII 187 CTTAAG C^TTAAG
AlwI 148 GATCC GGATC(4/5)
AocI 107 CCTTAGG CC^T[ACGT]AGG
AvrII 775 CCTAGG C^CTAGG
BalI 644 TGGCCA TGG^CCA
BbsI 329 GTCTTC GAAGAC(2/6)
BfrI 187 CTTAAG C^TTAAG
BglII 96 AGATCT A^GATCT
BlnI 775 CCTAGG C^CTAGG
BpuAI 329 GTCTTC GAAGAC(2/6)
BsaBI 9 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 677 GAATGC GAATGC(1/-1)
BstAPI 496 GCAGGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 225 CCATGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 96 AGATCT [GA]^GATC[CT]
Bsu36I 107 CCTTAGG CC^T[ACGT]AGG
Cac8I 747 GCCAGC GC[ACGT]^[ACGT]GC
DraI 603 TTTAAA TTT^AAA
DraIII 168 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 644 TGGCCA [CT]^GGCC[GA]
EarI 611 GAAGAG CTCTTC(1/4)
Eco81I 107 CCTTAGG CC^T[ACGT]AGG
EcoRI 549 GAATTC G^AATTC
EcoRV 785 GATATC GAT^ATC
FauI 50 GCGGG CCCGC(4/6)
HincII 471 GTTAAC GT[CT]^[GA]AC
HindII 471 GTTAAC GT[CT]^[GA]AC
HpaI 471 GTTAAC GTT^AAC
HphI 339 TCACC GGTGA(8/7)
Ksp632I 611 GAAGAG CTCTTC(1/4)
MamI 9 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 96 AGATCT [GA]^GATC[CT]
MluNI 644 TGGCCA TGG^CCA
MscI 644 TGGCCA TGG^CCA
MslI 118 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
SfaNI 456 GATGC GCATC(5/9)
SmlI 187 CTTAAG C^T[CT][GA]AG
SwaI 602 ATTTAAAT ATTT^AAAT
TaqI 92 TCGA T^CGA
TthHB8I 92 TCGA T^CGA
XhoII 96 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AvaII 53 GGTCC G^G[AT]CC
633 GGACC
BbvI 522 GCAGC GCAGC(8/12)
811 GCTGC
BglI 765 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
814 GCCATGGCGGC
BpmI 735 CTCCAG CTGGAG(16/14)
752 CTCCAG
BstF5I 830 CATCC GGATG(2/0)
8 GGATG
Cfr13I 53 GGTCC G^G[ACGT]CC
633 GGACC
DpnII 97 GATC ^GATC
148 GATC
DsaI 32 CCATGG C^C[GA][CT]GG
815 CCATGG
FokI 830 CATCC GGATG(9/13)
8 GGATG
HaeIII 645 GGCC GG^CC
773 GGCC
MboI 97 GATC ^GATC
148 GATC
MnlI 485 CCTC CCTC(7/6)
803 GAGG
NcoI 32 CCATGG C^CATGG
815 CCATGG
NdeII 97 GATC ^GATC
148 GATC
NlaIV 52 GGGTCC GG[ACGT]^[ACGT]CC
633 GGACCC
PleI 366 GACTC GAGTC(4/5)
37 GAGTC
Sau3AI 97 GATC ^GATC
148 GATC
Sau96I 53 GGTCC G^G[ACGT]CC
633 GGACC
TseI 522 GCAGC G^C[AT]GC
811 GCTGC
TspRI 172 CAGTG CA[GC]TG[ACGT][ACGT]^
300 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AcsI AluI ApoI BfaI
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstNI DdeI EcoRII EcoT14I
Fnu4HI HinfI ItaI MaeI MaeIII
MboII MseI MvaI MwoI NlaIII
ScrFI StyI TfiI Tru9I Tsp45I
Tsp509I