##################################
# NO CUT ENZYMES (  204 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BsaAI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       FauI        
FbaI        FokI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MluI        MluNI       MroI        
MscI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        ScrFI       SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        TaqI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AluI           29  AGCT                 AG^CT
BbsI           35  GAAGAC               GAAGAC(2/6)
BbvI          292  GCTGC                GCAGC(8/12)
BciVI         448  GTATCC               GTATCC(6/5)
BpmI          352  CTCCAG               CTGGAG(16/14)
BpuAI          35  GAAGAC               GAAGAC(2/6)
BsaBI         135  GATTTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          337  GGTCTC               GGTCTC(1/5)
BsmI          243  GCATTC               GAATGC(1/-1)
BsrDI         271  CATTGC               GCAATG(2/0)
BstYI         235  GGATCT               [GA]^GATC[CT]
Cac8I          42  GCAAGC               GC[ACGT]^[ACGT]GC
DraI          510  TTTAAA               TTT^AAA
DsaI           88  CCACGG               C^C[GA][CT]GG
EcoT14I       282  CCAAGG               C^C[AT][AT]GG
EcoT22I       268  ATGCAT               ATGCA^T
Fnu4HI        292  GCTGC                GC^[ACGT]GC
HinfI         391  GAATC                G^A[ACGT]TC
HphI          522  GGTGA                GGTGA(8/7)
ItaI          292  GCTGC                GC^[ACGT]GC
MamI          135  GATTTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          149  CAATTG               C^AATTG
MflI          235  GGATCT               [GA]^GATC[CT]
MslI          534  CATTCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          149  CAATTG               C^AATTG
NlaIII         76  CATG                 CATG^
NsiI          268  ATGCAT               ATGCA^T
NspI           75  GCATGT               [GA]CATG^[CT]
SfaNI         267  GATGC                GCATC(5/9)
SspI           66  AATATT               AAT^ATT
StyI          282  CCAAGG               C^C[AT][AT]GG
TfiI          391  GAATC                G^A[AT]TC
TseI          292  GCTGC                G^C[AT]GC
XhoII         235  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AcsI          298  GAATTT               [GA]^AATT[CT]
              306  AAATTC              
AlwI          439  GATCC                GGATC(4/5)
              235  GGATC               
ApoI          298  GAATTT               [GA]^AATT[CT]
              306  AAATTC              
BsaJI          88  CCACGG               C^C[ACGT][ACGT]GG
              282  CCAAGG              
BseRI          10  CTCCTC               GAGGAG(10/8)
               19  CTCCTC              
BsmAI         252  GTCTC                GTCTC(1/5)
              338  GTCTC               
DpnII         236  GATC                 ^GATC
              439  GATC                
MboI          236  GATC                 ^GATC
              439  GATC                
MseI           98  TTAA                 T^TAA
              511  TTAA                
NdeII         236  GATC                 ^GATC
              439  GATC                
Sau3AI        236  GATC                 ^GATC
              439  GATC                
Tru9I          98  TTAA                 T^TAA
              511  TTAA                
TspRI          24  CACTG                CA[GC]TG[ACGT][ACGT]^
              111  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

BsrI        DdeI        MaeIII      MboII       MnlI        
Tsp45I      Tsp509I