Restriction map for
pFN21AE6647
##################################
# NO CUT ENZYMES ( 187 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmFI BsoBI BspDI BspEI BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT14I FauI FbaI
Fnu4HI FseI FspI HaeII HaeIII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII ItaI KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
TaqI TseI TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 116 GTAGAC GT^[AC][GT]AC
AluI 135 AGCT AG^CT
AlwI 562 GGATC GGATC(4/5)
AlwNI 131 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 551 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 632 GTGCTC G[AT]GC[AT]^C
BbsI 356 GTCTTC GAAGAC(2/6)
BglI 341 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 632 GTGCTC G[AGT]GC[ACT]^C
BpuAI 356 GTCTTC GAAGAC(2/6)
BsaI 401 GGTCTC GGTCTC(1/5)
BsiHKAI 632 GTGCTC G[AT]GC[AT]^C
BsiYI 346 CCTGGCTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 346 CCTGGCTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 625 GCATTC GAATGC(1/-1)
Bsp1286I 632 GTGCTC G[AGT]GC[ACT]^C
BspHI 102 TCATGA T^CATGA
BsrDI 211 CATTGC GCAATG(2/0)
BstAPI 625 GCATTCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 230 GGTCACC G^GT[ACGT]ACC
BstPI 230 GGTCACC G^GT[ACGT]ACC
Cac8I 337 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 367 GCGC GCG^C
DpnII 563 GATC ^GATC
DraI 498 TTTAAA TTT^AAA
EcoO65I 230 GGTCACC G^GT[ACGT]ACC
EcoT22I 417 ATGCAT ATGCA^T
HhaI 367 GCGC GCG^C
HinP1I 367 GCGC G^CGC
HphI 232 TCACC GGTGA(8/7)
MboI 563 GATC ^GATC
NdeII 563 GATC ^GATC
NsiI 417 ATGCAT ATGCA^T
PleI 180 GACTC GAGTC(4/5)
RcaI 102 TCATGA T^CATGA
Sau3AI 563 GATC ^GATC
SspI 146 AATATT AAT^ATT
Tsp45I 231 GTCAC ^GT[GC]AC
XmnI 551 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 552 AAATTC [GA]^AATT[CT]
687 GAATTT
ApoI 552 AAATTC [GA]^AATT[CT]
687 GAATTT
Bsp1407I 312 TGTACA T^GTACA
652 TGTACA
BsrGI 312 TGTACA T^GTACA
652 TGTACA
BsrI 197 ACTGG ACTGG(1/-1)
130 CCAGT
BssKI 346 CCTGG ^CC[ACGT]GG
529 CCAGG
BstNI 346 CCTGG CC^[AT]GG
529 CCAGG
DdeI 250 CTCAG C^T[ACGT]AG
641 CTCAG
EcoRII 346 CCTGG ^CC[AT]GG
529 CCAGG
MnlI 485 CCTC CCTC(7/6)
92 GAGG
MvaI 346 CCTGG CC^[AT]GG
529 CCAGG
ScrFI 346 CCTGG CC^[ACGT]GG
529 CCAGG
SfaNI 416 GATGC GCATC(5/9)
419 GCATC
SfcI 137 CTGTAG C^T[GA][CT]AG
326 CTACAG
SspBI 312 TGTACA T^GTACA
652 TGTACA
TfiI 467 GATTC G^A[AT]TC
478 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AfaI AflIII BsmAI BspLU11I BstF5I
FokI HinfI MaeIII MboII MseI
MwoI NlaIII NspI RsaI Tru9I
Tsp509I