Restriction map for
pFN21AE5267
##################################
# NO CUT ENZYMES ( 191 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BcnI BfaI BglI BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssKI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT22I FauI Fnu4HI FseI FspI
HaeII HaeIII HgaI HhaI Hin1I
HinP1I HindIII HpaI ItaI KasI
KpnI Ksp632I KspI MaeI MaeII
MfeI MluI MluNI MscI MslI
MspA1I MunI MvaI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 109 TCCGGA T^CCGGA
AfaI 337 GTAC GT^AC
AflII 550 CTTAAG C^TTAAG
AluI 475 AGCT AG^CT
AlwI 204 GGATC GGATC(4/5)
AlwNI 293 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 368 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 73 GGACC G^G[AT]CC
BbsI 139 GAAGAC GAAGAC(2/6)
BclI 234 TGATCA T^GATCA
BfrI 550 CTTAAG C^TTAAG
BpuAI 139 GAAGAC GAAGAC(2/6)
BsaBI 229 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 366 GAGACC GGTCTC(1/5)
BsaJI 332 CCATGG C^C[ACGT][ACGT]GG
BsaWI 109 TCCGGA [AT]^CCGG[AT]
BseAI 109 TCCGGA T^CCGGA
BsmAI 366 GAGAC GTCTC(1/5)
BspEI 109 TCCGGA T^CCGGA
BssSI 185 CACGAG CACGAG(-5/-1)
Cfr13I 73 GGACC G^G[ACGT]CC
DsaI 332 CCATGG C^C[GA][CT]GG
EcoRI 172 GAATTC G^AATTC
EcoRV 216 GATATC GAT^ATC
EcoT14I 332 CCATGG C^C[AT][AT]GG
FbaI 234 TGATCA T^GATCA
HapII 110 CCGG C^CGG
HincII 285 GTTGAC GT[CT]^[GA]AC
HindII 285 GTTGAC GT[CT]^[GA]AC
HpaII 110 CCGG C^CGG
HphI 355 GGTGA GGTGA(8/7)
MaeIII 89 GTTAC ^GT[ACGT]AC
MamI 229 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MnlI 427 GAGG CCTC(7/6)
MroI 109 TCCGGA T^CCGGA
MspI 110 CCGG C^CGG
MwoI 532 GCACAACAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 332 CCATGG C^CATGG
PflFI 368 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 337 GTAC GT^AC
Sau96I 73 GGACC G^G[ACGT]CC
SfaNI 529 GATGC GCATC(5/9)
SmlI 550 CTTAAG C^T[CT][GA]AG
StyI 332 CCATGG C^C[AT][AT]GG
Tth111I 368 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AcsI 172 GAATTC [GA]^AATT[CT]
192 AAATTT
ApoI 172 GAATTC [GA]^AATT[CT]
192 AAATTT
BglII 81 AGATCT A^GATCT
322 AGATCT
BsrI 500 ACTGG ACTGG(1/-1)
516 CCAGT
BstF5I 243 CATCC GGATG(2/0)
62 GGATG
DdeI 85 CTGAG C^T[ACGT]AG
397 CTTAG
FokI 243 CATCC GGATG(9/13)
62 GGATG
HinfI 49 GAATC G^A[ACGT]TC
405 GATTC
MseI 319 TTAA T^TAA
551 TTAA
NlaIII 267 CATG CATG^
333 CATG
TfiI 49 GAATC G^A[AT]TC
405 GATTC
Tru9I 319 TTAA T^TAA
551 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
BstYI DpnII MboI MboII MflI
NdeII Sau3AI SfcI Tsp509I XhoII