Restriction map for
pFN21AE6559
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BanII BbrPI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeI MaeII MaeIII MamI MfeI
MluI MslI MspA1I MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 155 TCCGGA T^CCGGA
AcsI 283 AAATTC [GA]^AATT[CT]
AcyI 97 GGCGCC G[GA]^CG[CT]C
AfaI 135 GTAC GT^AC
AluI 435 AGCT AG^CT
AlwI 201 GATCC GGATC(4/5)
AlwNI 444 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 283 AAATTC [GA]^AATT[CT]
BbeI 97 GGCGCC GGCGC^C
BbsI 410 GTCTTC GAAGAC(2/6)
BbvCI 9 CCTCAGC CCTCAGC(-5/-2)
BglII 119 AGATCT A^GATCT
BpuAI 410 GTCTTC GAAGAC(2/6)
BsaHI 97 GGCGCC G[GA]^CG[CT]C
BsaI 72 GGTCTC GGTCTC(1/5)
BsaWI 155 TCCGGA [AT]^CCGG[AT]
BseAI 155 TCCGGA T^CCGGA
BsmFI 111 GTCCC GGGAC(10/14)
BspEI 155 TCCGGA T^CCGGA
BsrBI 314 CCGCTC CCGCTC(-3/-3)
BstAPI 443 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 21 CATCC GGATG(2/0)
BstXI 389 CCAACTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 321 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 98 GCGC GCG^C
DraII 235 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 235 GGGGCCT [GA]G^G[ACGT]CC[CT]
FokI 21 CATCC GGATG(9/13)
HaeII 97 GGCGCC [GA]GCGC^[CT]
HhaI 98 GCGC GCG^C
Hin1I 97 GGCGCC G[GA]^CG[CT]C
HinP1I 98 GCGC G^CGC
HphI 89 TCACC GGTGA(8/7)
KasI 97 GGCGCC G^GCGCC
MroI 155 TCCGGA T^CCGGA
MseI 265 TTAA T^TAA
NarI 97 GGCGCC GG^CGCC
NlaIII 293 CATG CATG^
RsaI 135 GTAC GT^AC
SfaNI 325 GCATC GCATC(5/9)
SfoI 97 GGCGCC GGC^GCC
TfiI 469 GATTC G^A[AT]TC
Tru9I 265 TTAA T^TAA
TspRI 406 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BalI 335 TGGCCA TGG^CCA
386 TGGCCA
BcnI 69 CCGGG CC^[GC]GG
92 CCGGG
BpmI 103 CTGGAG CTGGAG(16/14)
422 CTGGAG
BseRI 128 CTCCTC GAGGAG(10/8)
106 GAGGAG
BsiYI 301 CCTGGCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
415 CCCCAGGCTGG
BslI 301 CCTGGCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
415 CCCCAGGCTGG
BsmAI 73 GTCTC GTCTC(1/5)
402 GTCTC
BstYI 119 AGATCT [GA]^GATC[CT]
200 AGATCC
Cfr13I 6 GGCCC G^G[ACGT]CC
236 GGGCC
DdeI 10 CTCAG C^T[ACGT]AG
197 CTGAG
MflI 119 AGATCT [GA]^GATC[CT]
200 AGATCC
MluNI 335 TGGCCA TGG^CCA
386 TGGCCA
MscI 335 TGGCCA TGG^CCA
386 TGGCCA
NciI 69 CCGGG CC^[GC]GG
92 CCGGG
PstI 82 CTGCAG CTGCA^G
441 CTGCAG
Sau96I 6 GGCCC G^G[ACGT]CC
236 GGGCC
SfcI 82 CTGCAG C^T[GA][CT]AG
441 CTGCAG
XhoII 119 AGATCT [GA]^GATC[CT]
200 AGATCC
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI BanI BbvI BsaJI BssKI
BstNI DpnII EaeI EcoRII Fnu4HI
HaeIII HapII HinfI HpaII ItaI
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIV PleI Sau3AI
ScrFI TseI Tsp509I