Restriction map for
pFN21AE5288
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BamHI BbeI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspEI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
CpoI DpnII DraI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MaeIII MboI
MfeI MflI MluI MroI MseI
MslI MunI NaeI NarI NcoI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tth111I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 259 GTCTAC GT^[AC][GT]AC
AccII 618 CGCG CG^CG
AcyI 625 GACGCC G[GA]^CG[CT]C
AfaI 223 GTAC GT^AC
AlwNI 509 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 444 CCCGGG C^[CT]CG[GA]G
BanI 54 GGTGCC G^G[CT][GA]CC
BbrPI 331 CACGTG CAC^GTG
BbsI 160 GTCTTC GAAGAC(2/6)
BglI 360 GCCTCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 301 CTGGAG CTGGAG(16/14)
BpuAI 160 GTCTTC GAAGAC(2/6)
BsaBI 72 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 625 GACGCC G[GA]^CG[CT]C
BseRI 663 CTCCTC GAGGAG(10/8)
BsmI 65 GCATTC GAATGC(1/-1)
BsoBI 444 CCCGGG C^[CT]CG[GA]G
BspDI 79 ATCGAT AT^CGAT
BspHI 170 TCATGA T^CATGA
BsrFI 394 ACCGGC [GA]^CCGG[CT]
BssSI 568 CACGAG CACGAG(-5/-1)
BstF5I 232 CATCC GGATG(2/0)
BstUI 618 CGCG CG^CG
Cfr10I 394 ACCGGC [GA]^CCGG[CT]
ClaI 79 ATCGAT AT^CGAT
DraIII 586 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 399 CCAAGG C^C[AT][AT]GG
FauI 561 GCGGG CCCGC(4/6)
FokI 232 CATCC GGATG(9/13)
Hin1I 625 GACGCC G[GA]^CG[CT]C
MamI 72 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 559 CAGCGG C[AC]G^C[GT]G
MvnI 618 CGCG CG^CG
NdeI 681 CATATG CA^TATG
NspI 210 ACATGC [GA]CATG^[CT]
PmaCI 331 CACGTG CAC^GTG
PmlI 331 CACGTG CAC^GTG
RcaI 170 TCATGA T^CATGA
RsaI 223 GTAC GT^AC
SfcI 153 CTACAG C^T[GA][CT]AG
SmaI 444 CCCGGG CCC^GGG
SpeI 219 ACTAGT A^CTAGT
StyI 399 CCAAGG C^C[AT][AT]GG
TaqI 80 TCGA T^CGA
Tsp509I 118 AATT ^AATT
TthHB8I 80 TCGA T^CGA
XcmI 29 CCAGCCCCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 444 CCCGGG C^CCGGG
XmaI 444 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AciI 639 CCGC CCGC(-3/-1)
561 GCGG
AluI 527 AGCT AG^CT
596 AGCT
ApaI 441 GGGCCC GGGCC^C
554 GGGCCC
BalI 5 TGGCCA TGG^CCA
335 TGGCCA
BfaI 197 CTAG C^TAG
220 CTAG
BsaAI 37 TACGTG [CT]AC^GT[GA]
331 CACGTG
BsmFI 279 GTCCC GGGAC(10/14)
413 GTCCC
BsrI 105 ACTGG ACTGG(1/-1)
59 CCAGT
BstNI 508 CCAGG CC^[AT]GG
514 CCTGG
Cac8I 23 GCAGGC GC[ACGT]^[ACGT]GC
296 GCCTGC
CfoI 15 GCGC GCG^C
421 GCGC
DdeI 88 CTCAG C^T[ACGT]AG
540 CTCAG
EarI 634 CTCTTC CTCTTC(1/4)
652 CTCTTC
EcoRII 508 CCAGG ^CC[AT]GG
514 CCTGG
HgaI 625 GACGC GACGC(5/10)
158 GCGTC
HhaI 15 GCGC GCG^C
421 GCGC
HinP1I 15 GCGC G^CGC
421 GCGC
Ksp632I 634 CTCTTC CTCTTC(1/4)
652 CTCTTC
MaeI 197 CTAG C^TAG
220 CTAG
MluNI 5 TGGCCA TGG^CCA
335 TGGCCA
MscI 5 TGGCCA TGG^CCA
335 TGGCCA
MvaI 508 CCAGG CC^[AT]GG
514 CCTGG
PflMI 508 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
615 CCACGCGATGG
SapI 633 GCTCTTC GCTCTTC(1/4)
651 GCTCTTC
Van91I 508 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
615 CCACGCGATGG
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
BanII BbvI BcnI BmyI BsaI
BsaJI BsgI BsiYI BslI BsmAI
Bsp1286I BspMI BssKI Cfr13I DraII
EaeI EcoO109I Fnu4HI HaeIII HapII
HpaII HphI ItaI MaeII MboII
MnlI MspI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SfaNI TseI
TspRI