Restriction map for
pFN21AE5335
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanII BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstXI BstYI
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HgaI HindIII HpaI HphI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MspA1I MunI NaeI NciI NcoI
NdeI NgoMIV NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI Sau96I SbfI
ScaI SexAI SfaNI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tru9I Tsp45I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 411 GTATAC GT^[AC][GT]AC
AccII 75 CGCG CG^CG
AcyI 34 GGCGCC G[GA]^CG[CT]C
AfaI 423 GTAC GT^AC
AlwI 400 GGATC GGATC(4/5)
BanI 34 GGCGCC G^G[CT][GA]CC
BbeI 34 GGCGCC GGCGC^C
BbsI 469 GAAGAC GAAGAC(2/6)
BfaI 707 CTAG C^TAG
BpmI 332 CTGGAG CTGGAG(16/14)
BpuAI 469 GAAGAC GAAGAC(2/6)
BsaHI 34 GGCGCC G[GA]^CG[CT]C
BsiYI 427 CCAGAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 427 CCAGAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 755 GTCTC GTCTC(1/5)
BsmFI 116 GGGAC GGGAC(10/14)
BsmI 273 GAATGC GAATGC(1/-1)
BsrBI 21 GAGCGG CCGCTC(-3/-3)
Bst1107I 411 GTATAC GTA^TAC
BstUI 75 CGCG CG^CG
BstZ17I 411 GTATAC GTA^TAC
EaeI 5 CGGCCG [CT]^GGCC[GA]
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
HaeII 34 GGCGCC [GA]GCGC^[CT]
HapII 38 CCGG C^CGG
Hin1I 34 GGCGCC G[GA]^CG[CT]C
HincII 462 GTTGAC GT[CT]^[GA]AC
HindII 462 GTTGAC GT[CT]^[GA]AC
HpaII 38 CCGG C^CGG
KasI 34 GGCGCC G^GCGCC
MaeI 707 CTAG C^TAG
MaeII 654 ACGT A^CGT
MspI 38 CCGG C^CGG
MvnI 75 CGCG CG^CG
MwoI 10 GCCCTGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 34 GGCGCC GG^CGCC
NlaIII 288 CATG CATG^
NlaIV 34 GGCGCC GG[ACGT]^[ACGT]CC
NotI 4 GCGGCCGC GC^GGCCGC
RsaI 423 GTAC GT^AC
SfoI 34 GGCGCC GGC^GCC
SmlI 416 CTCAAG C^T[CT][GA]AG
TaqI 536 TCGA T^CGA
TthHB8I 536 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 240 AAATTT [GA]^AATT[CT]
559 GAATTT
ApoI 240 AAATTT [GA]^AATT[CT]
559 GAATTT
BbvI 507 GCAGC GCAGC(8/12)
100 GCTGC
BsaJI 11 CCCTGG C^C[ACGT][ACGT]GG
12 CCTGGG
BsiEI 5 CGGCCG CG[GA][CT]^CG
82 CGGTCG
BssKI 12 CCTGG ^CC[ACGT]GG
70 CCTGG
BstF5I 761 CATCC GGATG(2/0)
372 GGATG
BstNI 12 CCTGG CC^[AT]GG
70 CCTGG
Cac8I 19 GCGAGC GC[ACGT]^[ACGT]GC
31 GCAGGC
CfoI 35 GCGC GCG^C
74 GCGC
EcoRII 12 CCTGG ^CC[AT]GG
70 CCTGG
FokI 761 CATCC GGATG(9/13)
372 GGATG
HaeIII 6 GGCC GG^CC
448 GGCC
HhaI 35 GCGC GCG^C
74 GCGC
HinP1I 35 GCGC G^CGC
74 GCGC
MvaI 12 CCTGG CC^[AT]GG
70 CCTGG
ScrFI 12 CCTGG CC^[ACGT]GG
70 CCTGG
TseI 100 GCTGC G^C[AT]GC
507 GCAGC
TspRI 120 CAGTG CA[GC]TG[ACGT][ACGT]^
516 CACTG
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI BseRI BsrI DdeI
DpnII EarI Fnu4HI HinfI ItaI
Ksp632I MaeIII MboI MboII MnlI
NdeII SapI Sau3AI TfiI Tsp509I