Restriction map for
pFN21AE5773
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BbeI BbrPI BciVI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
FseI FspI HaeII HgaI HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1060 GACGTC GACGT^C
AcsI 744 AAATTC [GA]^AATT[CT]
AcyI 1060 GACGTC G[GA]^CG[CT]C
AlwNI 1354 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1698 GGGCCC GGGCC^C
ApoI 744 AAATTC [GA]^AATT[CT]
AseI 147 ATTAAT AT^TAAT
AsnI 147 ATTAAT AT^TAAT
AvaII 675 GGACC G^G[AT]CC
BamHI 894 GGATCC G^GATCC
BanI 86 GGCACC G^G[CT][GA]CC
BbvCI 160 CCTCAGC CCTCAGC(-5/-2)
BcgI 552 CGACTCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1343 TGATCA T^GATCA
BglII 584 AGATCT A^GATCT
BsaBI 763 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1060 GACGTC G[GA]^CG[CT]C
BsaI 347 GAGACC GGTCTC(1/5)
BsaWI 1394 ACCGGA [AT]^CCGG[AT]
BsgI 1369 CTGCAC GTGCAG(16/14)
BspMI 1367 ACCTGC ACCTGC(4/8)
BstBI 1766 TTCGAA TT^CGAA
BstXI 891 CCAGGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 1422 GCTAGC GC[ACGT]^[ACGT]GC
DraI 1459 TTTAAA TTT^AAA
EcoRV 1576 GATATC GAT^ATC
EcoT14I 839 CCTTGG C^C[AT][AT]GG
EcoT22I 691 ATGCAT ATGCA^T
FauI 1217 CCCGC CCCGC(4/6)
FbaI 1343 TGATCA T^GATCA
Hin1I 1060 GACGTC G[GA]^CG[CT]C
MamI 763 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 529 CATACCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 1422 GCTAGC G^CTAGC
NsiI 691 ATGCAT ATGCA^T
NspI 689 ACATGC [GA]CATG^[CT]
NspV 1766 TTCGAA TT^CGAA
PshAI 779 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 147 ATTAAT AT^TAAT
PstI 1052 CTGCAG CTGCA^G
SapI 568 GCTCTTC GCTCTTC(1/4)
ScaI 1529 AGTACT AGT^ACT
SfaNI 1759 GATGC GCATC(5/9)
SfcI 1052 CTGCAG C^T[GA][CT]AG
SfuI 1766 TTCGAA TT^CGAA
SspI 713 AATATT AAT^ATT
StuI 1638 AGGCCT AGG^CCT
StyI 839 CCTTGG C^C[AT][AT]GG
XcmI 1608 CCAACAGGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 54 GTAC GT^AC
1530 GTAC
Asp700I 565 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
662 GAAATGATTC
BalI 644 TGGCCA TGG^CCA
1650 TGGCCA
BanII 463 GAGCCC G[GA]GC[CT]^C
1698 GGGCCC
BbvI 1356 GCAGC GCAGC(8/12)
1589 GCAGC
BfaI 1423 CTAG C^TAG
1586 CTAG
BstYI 584 AGATCT [GA]^GATC[CT]
894 GGATCC
EaeI 644 TGGCCA [CT]^GGCC[GA]
1650 TGGCCA
Fnu4HI 1356 GCAGC GC^[ACGT]GC
1589 GCAGC
HapII 1395 CCGG C^CGG
1689 CCGG
HpaII 1395 CCGG C^CGG
1689 CCGG
ItaI 1356 GCAGC GC^[ACGT]GC
1589 GCAGC
MaeI 1423 CTAG C^TAG
1586 CTAG
MaeII 491 ACGT A^CGT
1061 ACGT
MflI 584 AGATCT [GA]^GATC[CT]
894 GGATCC
MluNI 644 TGGCCA TGG^CCA
1650 TGGCCA
MscI 644 TGGCCA TGG^CCA
1650 TGGCCA
MspA1I 224 CAGCTG C[AC]G^C[GT]G
1357 CAGCTG
MspI 1395 CCGG C^CGG
1689 CCGG
PvuII 224 CAGCTG CAG^CTG
1357 CAGCTG
RsaI 54 GTAC GT^AC
1530 GTAC
TfiI 667 GATTC G^A[AT]TC
811 GATTC
TseI 1356 GCAGC G^C[AT]GC
1589 GCAGC
Tsp509I 146 AATT ^AATT
745 AATT
XhoII 584 AGATCT [GA]^GATC[CT]
894 GGATCC
XmnI 565 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
662 GAAATGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwI AocI BbsI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Bsu36I
Cfr13I DdeI DpnII DraII EarI
Eco81I EcoO109I EcoRII FokI HaeIII
HinfI HphI Ksp632I MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI TaqI Tru9I Tsp45I
TspRI TthHB8I