Restriction map for
pFN21AE5776
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# NO CUT ENZYMES ( 97 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AocI
ApaI AscI Asp718I AspEI AvrII
BamHI BanII BbrPI BbvCI BcgI
BfrI BglI BlnI BsaAI BsaWI
BseAI BsiEI BsiWI BspEI BspLU11I
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FseI HincII HindII
HindIII HpaI KpnI KspI MaeII
MfeI MluI MroI MspA1I MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
StuI SwaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AccII 48 CGCG CG^CG
AcyI 1334 GGCGCC G[GA]^CG[CT]C
Alw44I 1270 GTGCAC G^TGCAC
AlwNI 362 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1049 AGCGCT AGC^GCT
ApaLI 1270 GTGCAC G^TGCAC
AseI 775 ATTAAT AT^TAAT
AsnI 775 ATTAAT AT^TAAT
AspI 631 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1772 TGCGCA TGC^GCA
BbeI 1334 GGCGCC GGCGC^C
BciVI 1299 GGATAC GTATCC(6/5)
BclI 1179 TGATCA T^GATCA
BfaI 1318 CTAG C^TAG
BglII 1137 AGATCT A^GATCT
BlpI 36 GCTGAGC GC^T[ACGT]AGC
BpmI 182 CTGGAG CTGGAG(16/14)
Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC
BsaBI 1180 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1334 GGCGCC G[GA]^CG[CT]C
BsgI 799 GTGCAG GTGCAG(16/14)
BsmBI 1450 GAGACG CGTCTC(1/5)
BsmFI 133 GGGAC GGGAC(10/14)
Bsp1407I 967 TGTACA T^GTACA
BspDI 562 ATCGAT AT^CGAT
BspMI 801 GCAGGT ACCTGC(4/8)
BsrBI 185 GAGCGG CCGCTC(-3/-3)
BsrDI 1516 CATTGC GCAATG(2/0)
BsrFI 1331 ACCGGC [GA]^CCGG[CT]
BsrGI 967 TGTACA T^GTACA
BstUI 48 CGCG CG^CG
CelII 36 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1331 ACCGGC [GA]^CCGG[CT]
ClaI 562 ATCGAT AT^CGAT
DraI 1219 TTTAAA TTT^AAA
DraII 587 AGGCCCC [GA]G^G[ACGT]CC[CT]
EarI 319 GAAGAG CTCTTC(1/4)
Eco47III 1049 AGCGCT AGC^GCT
EcoO109I 587 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 686 ATGCAT ATGCA^T
FauI 187 GCGGG CCCGC(4/6)
FbaI 1179 TGATCA T^GATCA
FspI 1772 TGCGCA TGC^GCA
HgaI 332 GACGC GACGC(5/10)
Hin1I 1334 GGCGCC G[GA]^CG[CT]C
KasI 1334 GGCGCC G^GCGCC
Ksp632I 319 GAAGAG CTCTTC(1/4)
MaeI 1318 CTAG C^TAG
MamI 1180 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 48 CGCG CG^CG
NarI 1334 GGCGCC GG^CGCC
NsiI 686 ATGCAT ATGCA^T
PaeR7I 1784 CTCGAG C^TCGAG
PflFI 631 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 775 ATTAAT AT^TAAT
PstI 1418 CTGCAG CTGCA^G
ScaI 857 AGTACT AGT^ACT
SexAI 224 ACCTGGT A^CC[AT]GGT
SfoI 1334 GGCGCC GGC^GCC
SmlI 1784 CTCGAG C^T[CT][GA]AG
SspBI 967 TGTACA T^GTACA
SspI 268 AATATT AAT^ATT
Tth111I 631 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1317 TCTAGA T^CTAGA
XcmI 1353 CCACCCTCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1784 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
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Asp700I 1432 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1666 GAAATAGTTC
BalI 1004 TGGCCA TGG^CCA
1463 TGGCCA
BanI 1334 GGCGCC G^G[CT][GA]CC
1624 GGTGCC
BsaI 1565 GAGACC GGTCTC(1/5)
280 GGTCTC
BspHI 435 TCATGA T^CATGA
939 TCATGA
BstAPI 340 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
792 GCAGTGGGTGC
BstNI 225 CCTGG CC^[AT]GG
1648 CCTGG
EaeI 1004 TGGCCA [CT]^GGCC[GA]
1463 TGGCCA
EcoRII 225 CCTGG ^CC[AT]GG
1648 CCTGG
HaeII 1049 AGCGCT [GA]GCGC^[CT]
1334 GGCGCC
MaeIII 82 GTCAC ^GT[ACGT]AC
263 GTGAC
MluNI 1004 TGGCCA TGG^CCA
1463 TGGCCA
MscI 1004 TGGCCA TGG^CCA
1463 TGGCCA
MslI 436 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
487 CATGTTTGTG
MvaI 225 CCTGG CC^[AT]GG
1648 CCTGG
RcaI 435 TCATGA T^CATGA
939 TCATGA
SmaI 10 CCCGGG CCC^GGG
530 CCCGGG
SphI 1413 GCATGC GCATG^C
1577 GCATGC
TfiI 565 GATTC G^A[AT]TC
1443 GAATC
Tsp45I 82 GTCAC ^GT[GC]AC
263 GTGAC
XmaCI 10 CCCGGG C^CCGGG
530 CCCGGG
XmaI 10 CCCGGG C^CCGGG
530 CCCGGG
XmnI 1432 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1666 GAAATAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BbsI
BbvI BcnI BmyI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmI BsoBI Bsp1286I BsrI BssKI
BstF5I BstYI Cac8I CfoI Cfr13I
DdeI DpnII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MflI MnlI MseI MspI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I
Van91I XhoII