Restriction map for
pFN21AE5819
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccI AccIII Alw44I AlwI
Aor51HI ApaLI AscI AseI AsnI
AspI AviII AvrII BamHI BclI
BglII BlnI BsaBI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FbaI FseI
FspI HindIII HpaI MamI MfeI
MflI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PmeI
PshBI PvuI RsrII SalI SapI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Tth111I XbaI
XhoI XhoII
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1794 GGTACC G^GTACC
AclI 2446 AACGTT AA^CGTT
AcsI 1219 GAATTT [GA]^AATT[CT]
AflII 2584 CTTAAG C^TTAAG
AflIII 1788 ACACGT A^C[GA][CT]GT
AgeI 661 ACCGGT A^CCGGT
AhdI 2535 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 177 GGGCCC GGGCC^C
ApoI 1219 GAATTT [GA]^AATT[CT]
Asp718I 1794 GGTACC G^GTACC
AspEI 2535 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2077 GGCGCC GGCGC^C
BbrPI 1789 CACGTG CAC^GTG
BbvCI 797 CCTCAGC CCTCAGC(-5/-2)
BciVI 2000 GGATAC GTATCC(6/5)
BfrI 2584 CTTAAG C^TTAAG
BsaAI 1789 CACGTG [CT]AC^GT[GA]
BsmBI 958 GAGACG CGTCTC(1/5)
BsrBI 2159 CCGCTC CCGCTC(-3/-3)
DpnII 2919 GATC ^GATC
Eam1105I 2535 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 1426 GTCAAC GT[CT]^[GA]AC
HindII 1426 GTCAAC GT[CT]^[GA]AC
KasI 2077 GGCGCC G^GCGCC
KpnI 1794 GGTACC GGTAC^C
MboI 2919 GATC ^GATC
NarI 2077 GGCGCC GG^CGCC
NcoI 376 CCATGG C^CATGG
NdeII 2919 GATC ^GATC
PflMI 528 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 661 ACCGGT A^CCGGT
PmaCI 1789 CACGTG CAC^GTG
PmlI 1789 CACGTG CAC^GTG
PshAI 1273 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2446 AACGTT AA^CGTT
Sau3AI 2919 GATC ^GATC
SbfI 519 CCTGCAGG CCTGCA^GG
ScaI 1481 AGTACT AGT^ACT
SfiI 424 GGCCCCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2077 GGCGCC GGC^GCC
Sse8387I 519 CCTGCAGG CCTGCA^GG
Van91I 528 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 461 CCACCACGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 123 GGCGTC G[GA]^CG[CT]C
2077 GGCGCC
AocI 382 CCTCAGG CC^T[ACGT]AGG
2143 CCTGAGG
Asp700I 1647 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2684 GAAATAATTC
BcgI 957 CGAGACGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2337 GCACCTGGGTCG
BglI 425 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
802 GCCTCTACGGC
BsaHI 123 GGCGTC G[GA]^CG[CT]C
2077 GGCGCC
BsaI 940 GAGACC GGTCTC(1/5)
3112 GGTCTC
BsaWI 661 ACCGGT [AT]^CCGG[AT]
2273 TCCGGT
BspHI 1281 TCATGA T^CATGA
1529 TCATGA
BsrDI 1799 CATTGC GCAATG(2/0)
3173 GCAATG
BsrFI 661 ACCGGT [GA]^CCGG[CT]
2390 ACCGGC
Bsu36I 382 CCTCAGG CC^T[ACGT]AGG
2143 CCTGAGG
Cfr10I 661 ACCGGT [GA]^CCGG[CT]
2390 ACCGGC
Eco81I 382 CCTCAGG CC^T[ACGT]AGG
2143 CCTGAGG
EcoT22I 2046 ATGCAT ATGCA^T
2991 ATGCAT
HaeII 2077 GGCGCC [GA]GCGC^[CT]
3178 GGCGCT
HgaI 616 GACGC GACGC(5/10)
124 GCGTC
Hin1I 123 GGCGTC G[GA]^CG[CT]C
2077 GGCGCC
KspI 2116 CCGCGG CCGC^GG
2843 CCGCGG
MseI 845 TTAA T^TAA
2585 TTAA
NsiI 2046 ATGCAT ATGCA^T
2991 ATGCAT
NspI 21 GCATGT [GA]CATG^[CT]
2762 ACATGC
PpuMI 1559 AGGACCT [GA]G^G[AT]CC[CT]
2713 GGGACCC
RcaI 1281 TCATGA T^CATGA
1529 TCATGA
SacI 718 GAGCTC GAGCT^C
3009 GAGCTC
SacII 2116 CCGCGG CCGC^GG
2843 CCGCGG
TaqI 1667 TCGA T^CGA
2984 TCGA
Tru9I 845 TTAA T^TAA
2585 TTAA
TthHB8I 1667 TCGA T^CGA
2984 TCGA
XmnI 1647 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2684 GAAATAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CelII CfoI
Cfr13I DdeI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI PstI
PvuII RsaI Sau96I ScrFI SexAI
SfaNI SfcI SmaI SmlI StuI
StyI TfiI TseI Tsp45I Tsp509I
TspRI XmaCI XmaI