Restriction map for
pFN21AE5154
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI Ksp632I KspI MaeI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp45I TspRI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 104 GGTACC G^GTACC
AluI 218 AGCT AG^CT
Asp700I 12 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 104 GGTACC G^GTACC
BanII 274 GAGCCC G[GA]GC[CT]^C
BbsI 96 GAAGAC GAAGAC(2/6)
BcnI 268 CCGGG CC^[GC]GG
BglI 174 GCCCAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 57 AGATCT A^GATCT
BmyI 274 GAGCCC G[AGT]GC[ACT]^C
BpuAI 96 GAAGAC GAAGAC(2/6)
BsmAI 162 GTCTC GTCTC(1/5)
BsmFI 69 GTCCC GGGAC(10/14)
Bsp1286I 274 GAGCCC G[AGT]GC[ACT]^C
BspMI 90 GCAGGT ACCTGC(4/8)
BsrFI 255 ACCGGC [GA]^CCGG[CT]
BsrI 84 CCAGT ACTGG(1/-1)
BssHII 234 GCGCGC G^CGCGC
BstYI 57 AGATCT [GA]^GATC[CT]
Cfr10I 255 ACCGGC [GA]^CCGG[CT]
DpnII 58 GATC ^GATC
HphI 93 GGTGA GGTGA(8/7)
KpnI 104 GGTACC GGTAC^C
MaeII 100 ACGT A^CGT
MboI 58 GATC ^GATC
MflI 57 AGATCT [GA]^GATC[CT]
NciI 268 CCGGG CC^[GC]GG
NdeII 58 GATC ^GATC
PstI 142 CTGCAG CTGCA^G
Sau3AI 58 GATC ^GATC
SfaNI 244 GATGC GCATC(5/9)
SfcI 142 CTGCAG C^T[GA][CT]AG
TaqI 20 TCGA T^CGA
TfiI 111 GAATC G^A[AT]TC
Tsp509I 201 AATT ^AATT
TthHB8I 20 TCGA T^CGA
XhoII 57 AGATCT [GA]^GATC[CT]
XmnI 12 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccII 235 CGCG CG^CG
292 CGCG
AfaI 33 GTAC GT^AC
105 GTAC
BanI 104 GGTACC G^G[CT][GA]CC
264 GGTGCC
BbvI 216 GCAGC GCAGC(8/12)
141 GCTGC
BpmI 7 CTGGAG CTGGAG(16/14)
157 CTGGAG
BstF5I 119 GGATG GGATG(2/0)
243 GGATG
BstUI 235 CGCG CG^CG
292 CGCG
CfoI 234 GCGC GCG^C
236 GCGC
EcoT14I 176 CCAAGG C^C[AT][AT]GG
239 CCAAGG
FokI 119 GGATG GGATG(9/13)
243 GGATG
HhaI 234 GCGC GCG^C
236 GCGC
HinP1I 234 GCGC G^CGC
236 GCGC
MvnI 235 CGCG CG^CG
292 CGCG
PleI 67 GAGTC GAGTC(4/5)
160 GAGTC
RsaI 33 GTAC GT^AC
105 GTAC
StyI 176 CCAAGG C^C[AT][AT]GG
239 CCAAGG
TseI 141 GCTGC G^C[AT]GC
216 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI BsaJI BsiYI BslI BssKI
BstNI Cac8I Cfr13I DdeI EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MboII MnlI MspI MvaI
MwoI NlaIV Sau96I ScrFI