Restriction map for
pFN21AE6273
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII AccI AccIII AclI AflII
AflIII AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspHI AspI
AvaI AvaII AvrII BamHI BanII
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstZ17I Bsu36I
CelII Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FokI
FseI HincII HindII HindIII HpaI
Ksp632I KspI MaeI MamI MfeI
MluI MroI MseI MspA1I MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SfcI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
StuI SwaI TaqI Tru9I Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 549 GGTACC G^GTACC
AcsI 139 GAATTT [GA]^AATT[CT]
AgeI 552 ACCGGT A^CCGGT
ApoI 139 GAATTT [GA]^AATT[CT]
Asp700I 3 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 549 GGTACC G^GTACC
AviII 332 TGCGCA TGC^GCA
BalI 479 TGGCCA TGG^CCA
BbeI 301 GGCGCC GGCGC^C
BbvCI 544 GCTGAGG CCTCAGC(-5/-2)
BglII 338 AGATCT A^GATCT
BsaI 507 GGTCTC GGTCTC(1/5)
BsaWI 552 ACCGGT [AT]^CCGG[AT]
BstNI 279 CCTGG CC^[AT]GG
BstYI 338 AGATCT [GA]^GATC[CT]
DdeI 545 CTGAG C^T[ACGT]AG
EaeI 479 TGGCCA [CT]^GGCC[GA]
EcoRII 279 CCTGG ^CC[AT]GG
EcoT14I 134 CCAAGG C^C[AT][AT]GG
FspI 332 TGCGCA TGC^GCA
HaeII 301 GGCGCC [GA]GCGC^[CT]
HinfI 384 GATTC G^A[ACGT]TC
HphI 435 GGTGA GGTGA(8/7)
KasI 301 GGCGCC G^GCGCC
KpnI 549 GGTACC GGTAC^C
MaeII 204 ACGT A^CGT
MaeIII 292 GTGAC ^GT[ACGT]AC
MflI 338 AGATCT [GA]^GATC[CT]
MluNI 479 TGGCCA TGG^CCA
MscI 479 TGGCCA TGG^CCA
MvaI 279 CCTGG CC^[AT]GG
NaeI 347 GCCGGC GCC^GGC
NarI 301 GGCGCC GG^CGCC
NgoMIV 347 GCCGGC G^CCGGC
NspI 329 GCATGC [GA]CATG^[CT]
PflMI 75 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 552 ACCGGT A^CCGGT
SexAI 278 ACCTGGT A^CC[AT]GGT
SfaNI 541 GATGC GCATC(5/9)
SfoI 301 GGCGCC GGC^GCC
SphI 329 GCATGC GCATG^C
SspI 472 AATATT AAT^ATT
StyI 134 CCAAGG C^C[AT][AT]GG
TfiI 384 GATTC G^A[AT]TC
Tsp45I 292 GTGAC ^GT[GC]AC
Van91I 75 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 338 AGATCT [GA]^GATC[CT]
XmnI 3 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AciI 325 CCGC CCGC(-3/-1)
226 GCGG
AcyI 151 GACGCC G[GA]^CG[CT]C
301 GGCGCC
AfaI 420 GTAC GT^AC
550 GTAC
AluI 242 AGCT AG^CT
495 AGCT
BbvI 493 GCAGC GCAGC(8/12)
243 GCTGC
BcnI 296 CCGGG CC^[GC]GG
516 CCGGG
BsaHI 151 GACGCC G[GA]^CG[CT]C
301 GGCGCC
BsaJI 134 CCAAGG C^C[ACGT][ACGT]GG
296 CCGGGG
BseRI 466 GAGGAG GAGGAG(10/8)
565 GAGGAG
BsmAI 175 GAGAC GTCTC(1/5)
508 GTCTC
BsmFI 518 GGGAC GGGAC(10/14)
558 GGGAC
BsrFI 347 GCCGGC [GA]^CCGG[CT]
552 ACCGGT
BsrI 180 ACTGG ACTGG(1/-1)
257 ACTGG
Cfr10I 347 GCCGGC [GA]^CCGG[CT]
552 ACCGGT
DpnII 96 GATC ^GATC
339 GATC
HaeIII 409 GGCC GG^CC
480 GGCC
HgaI 151 GACGC GACGC(5/10)
223 GACGC
Hin1I 151 GACGCC G[GA]^CG[CT]C
301 GGCGCC
MboI 96 GATC ^GATC
339 GATC
MboII 186 GAAGA GAAGA(8/7)
341 TCTTC
MslI 24 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
285 CATTTCTGTG
NciI 296 CCGGG CC^[GC]GG
516 CCGGG
NdeII 96 GATC ^GATC
339 GATC
RsaI 420 GTAC GT^AC
550 GTAC
Sau3AI 96 GATC ^GATC
339 GATC
TseI 243 GCTGC G^C[AT]GC
493 GCAGC
Tsp509I 140 AATT ^AATT
535 AATT
TspRI 179 CACTG CA[GC]TG[ACGT][ACGT]^
256 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AccII BanI BsiYI BslI BssKI
BstUI Cac8I CfoI Fnu4HI HapII
HhaI HinP1I HpaII ItaI MnlI
MspI MvnI MwoI NlaIII NlaIV
ScrFI