Restriction map for
pFN21AE5449
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BbeI BbrPI
BcgI BciVI BglI BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FauI FokI
FseI FspI HgaI Hin1I HincII
HindII HpaI HphI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 300 GTCTAC GT^[AC][GT]AC
AcsI 39 GAATTT [GA]^AATT[CT]
AflII 30 CTTAAG C^TTAAG
AlwNI 941 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 26 AGCGCT AGC^GCT
ApoI 39 GAATTT [GA]^AATT[CT]
AseI 769 ATTAAT AT^TAAT
AsnI 769 ATTAAT AT^TAAT
BamHI 158 GGATCC G^GATCC
BbvCI 938 CCTCAGC CCTCAGC(-5/-2)
BclI 363 TGATCA T^GATCA
BfaI 274 CTAG C^TAG
BfrI 30 CTTAAG C^TTAAG
BglII 579 AGATCT A^GATCT
BlpI 22 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 22 GCTGAGC GC^T[ACGT]AGC
BsgI 794 CTGCAC GTGCAG(16/14)
BsmAI 574 GAGAC GTCTC(1/5)
BsmFI 753 GGGAC GGGAC(10/14)
BssSI 193 CTCGTG CACGAG(-5/-1)
BstXI 473 CCAAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 22 GCTGAGC GC^T[ACGT]AGC
CfoI 27 GCGC GCG^C
DraI 16 TTTAAA TTT^AAA
DraII 621 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 203 CCATGG C^C[GA][CT]GG
Eco47III 26 AGCGCT AGC^GCT
EcoO109I 621 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 203 CCATGG C^C[AT][AT]GG
FbaI 363 TGATCA T^GATCA
HaeII 26 AGCGCT [GA]GCGC^[CT]
HhaI 27 GCGC GCG^C
HinP1I 27 GCGC G^CGC
HindIII 838 AAGCTT A^AGCTT
MaeI 274 CTAG C^TAG
MaeII 521 ACGT A^CGT
MaeIII 227 GTGAC ^GT[ACGT]AC
NcoI 203 CCATGG C^CATGG
PflMI 658 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 326 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 769 ATTAAT AT^TAAT
PstI 854 CTGCAG CTGCA^G
SacI 152 GAGCTC GAGCT^C
SapI 985 GAAGAGC GCTCTTC(1/4)
SfcI 854 CTGCAG C^T[GA][CT]AG
SmlI 30 CTTAAG C^T[CT][GA]AG
StyI 203 CCATGG C^C[AT][AT]GG
TaqI 324 TCGA T^CGA
Tsp45I 227 GTGAC ^GT[GC]AC
TspRI 743 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 324 TCGA T^CGA
Van91I 658 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 222 GTAC GT^AC
523 GTAC
AspHI 152 GAGCTC G[AT]GC[AT]^C
169 GAGCAC
BanI 681 GGTGCC G^G[CT][GA]CC
928 GGTGCC
BanII 6 GGGCTC G[GA]GC[CT]^C
152 GAGCTC
BbsI 184 GAAGAC GAAGAC(2/6)
949 GAAGAC
BcnI 180 CCGGG CC^[GC]GG
974 CCCGG
BpmI 328 CTCCAG CTGGAG(16/14)
214 CTGGAG
BpuAI 184 GAAGAC GAAGAC(2/6)
949 GAAGAC
BsiHKAI 152 GAGCTC G[AT]GC[AT]^C
169 GAGCAC
BspMI 230 ACCTGC ACCTGC(4/8)
852 ACCTGC
DrdI 538 GACTCTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
868 GACAACTTTGTC
EcoNI 192 CCTCGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
625 CCTGGAAGAGG
HapII 180 CCGG C^CGG
975 CCGG
HpaII 180 CCGG C^CGG
975 CCGG
MspI 180 CCGG C^CGG
975 CCGG
MwoI 97 GCCCAGAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
891 GCCATCTGTGC
NciI 180 CCGGG CC^[GC]GG
974 CCCGG
PleI 326 GACTC GAGTC(4/5)
538 GACTC
RsaI 222 GTAC GT^AC
523 GTAC
SfaNI 919 GCATC GCATC(5/9)
943 GCATC
XcmI 203 CCATGGAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
473 CCAAAATCCTGGTGG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AluI AlwI AvaII BbvI BmyI
BsaJI BseRI BsiYI BslI Bsp1286I
BsrI BssKI BstNI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI HaeIII HinfI ItaI Ksp632I
MboI MboII MflI MnlI MseI
MslI MvaI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TfiI Tru9I
TseI Tsp509I XhoII