Restriction map for
pFN21AE9690
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
AhdI ApaI AscI AspEI BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoRV FbaI FseI HapII
HgaI HpaI HpaII KasI KspI
MamI MluI MroI MspI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 2840 GACGTC GACGT^C
Acc65I 1666 GGTACC G^GTACC
AccI 1922 GTCGAC GT^[AC][GT]AC
AccII 2294 CGCG CG^CG
AcsI 187 GAATTC [GA]^AATT[CT]
AcyI 2840 GACGTC G[GA]^CG[CT]C
Alw44I 533 GTGCAC G^TGCAC
AocI 875 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1222 AGCGCT AGC^GCT
ApaLI 533 GTGCAC G^TGCAC
ApoI 187 GAATTC [GA]^AATT[CT]
Asp718I 1666 GGTACC G^GTACC
AspI 2376 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1787 CCCGAG C^[CT]CG[GA]G
AviII 1875 TGCGCA TGC^GCA
AvrII 363 CCTAGG C^CTAGG
BalI 1803 TGGCCA TGG^CCA
BamHI 104 GGATCC G^GATCC
BglI 1119 GCCCTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1033 AGATCT A^GATCT
BlnI 363 CCTAGG C^CTAGG
BsaHI 2840 GACGTC G[GA]^CG[CT]C
BsmBI 2838 GAGACG CGTCTC(1/5)
BsoBI 1787 CCCGAG C^[CT]CG[GA]G
BstUI 2294 CGCG CG^CG
BstXI 291 CCAGCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 875 CCTCAGG CC^T[ACGT]AGG
DraII 2616 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 1084 CCACGG C^C[GA][CT]GG
Eco47III 1222 AGCGCT AGC^GCT
Eco81I 875 CCTCAGG CC^T[ACGT]AGG
EcoNI 662 CCTCACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2616 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 187 GAATTC G^AATTC
EcoT22I 1484 ATGCAT ATGCA^T
FspI 1875 TGCGCA TGC^GCA
HaeII 1222 AGCGCT [GA]GCGC^[CT]
Hin1I 2840 GACGTC G[GA]^CG[CT]C
KpnI 1666 GGTACC GGTAC^C
MaeII 2841 ACGT A^CGT
MfeI 2699 CAATTG C^AATTG
MluNI 1803 TGGCCA TGG^CCA
MscI 1803 TGGCCA TGG^CCA
MunI 2699 CAATTG C^AATTG
MvnI 2294 CGCG CG^CG
NdeI 2913 CATATG CA^TATG
NsiI 1484 ATGCAT ATGCA^T
PflFI 2376 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2338 CCAAACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2616 AGGTCCT [GA]G^G[AT]CC[CT]
SalI 1922 GTCGAC G^TCGAC
SapI 2533 GCTCTTC GCTCTTC(1/4)
SbfI 148 CCTGCAGG CCTGCA^GG
SexAI 2463 ACCTGGT A^CC[AT]GGT
Sse8387I 148 CCTGCAGG CCTGCA^GG
SspI 342 AATATT AAT^ATT
StuI 1262 AGGCCT AGG^CCT
Tth111I 2376 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2338 CCAAACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2912 CCATATGCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
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AlwNI 566 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
2224 CAGAGACTG
Asp700I 183 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2641 GAACTGATTC
BanI 1666 GGTACC G^G[CT][GA]CC
2112 GGCACC
BbsI 2779 GAAGAC GAAGAC(2/6)
2843 GTCTTC
BbvCI 780 CCTCAGC CCTCAGC(-5/-2)
2158 CCTCAGC
BpuAI 2779 GAAGAC GAAGAC(2/6)
2843 GTCTTC
BsaI 539 GGTCTC GGTCTC(1/5)
1007 GGTCTC
Bsp1407I 2061 TGTACA T^GTACA
2710 TGTACA
BspHI 1677 TCATGA T^CATGA
2238 TCATGA
BsrGI 2061 TGTACA T^GTACA
2710 TGTACA
BstYI 104 GGATCC [GA]^GATC[CT]
1033 AGATCT
CfoI 1223 GCGC GCG^C
1876 GCGC
EaeI 1072 CGGCCA [CT]^GGCC[GA]
1803 TGGCCA
FauI 1989 CCCGC CCCGC(4/6)
2295 GCGGG
HhaI 1223 GCGC GCG^C
1876 GCGC
HinP1I 1223 GCGC G^CGC
1876 GCGC
HindIII 911 AAGCTT A^AGCTT
1976 AAGCTT
MflI 104 GGATCC [GA]^GATC[CT]
1033 AGATCT
NspI 1931 ACATGC [GA]CATG^[CT]
2064 ACATGC
PstI 149 CTGCAG CTGCA^G
2447 CTGCAG
RcaI 1677 TCATGA T^CATGA
2238 TCATGA
SacI 28 GAGCTC GAGCT^C
2531 GAGCTC
SspBI 2061 TGTACA T^GTACA
2710 TGTACA
XhoII 104 GGATCC [GA]^GATC[CT]
1033 AGATCT
XmnI 183 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2641 GAACTGATTC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AseI
AsnI AspHI AvaII BanII BbvI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrDI BsrI
BssKI BstEII BstF5I BstNI BstPI
Cac8I Cfr13I DdeI DpnII DraIII
EarI EcoO65I EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PshBI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I