Restriction map for
pFN21AE6181
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# NO CUT ENZYMES ( 176 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglI BglII BlnI BpmI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstNI
BstPI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DpnII DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRII EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI MaeI MaeII MaeIII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MslI MunI MvaI
MwoI NaeI NarI NcoI NdeI
NdeII NgoMIV NheI NlaIII NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SalI SapI Sau3AI SbfI
SexAI SfiI SfoI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 34 CGCG CG^CG
AfaI 192 GTAC GT^AC
Aor51HI 114 AGCGCT AGC^GCT
AvaII 13 GGTCC G^G[AT]CC
BbvI 142 GCAGC GCAGC(8/12)
BcgI 63 GCACTCCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 117 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 117 GCTTAGC GC^T[ACGT]AGC
BsmAI 305 GAGAC GTCTC(1/5)
BsrI 194 ACTGG ACTGG(1/-1)
BstBI 256 TTCGAA TT^CGAA
BstUI 34 CGCG CG^CG
Cac8I 74 GCCTGC GC[ACGT]^[ACGT]GC
CelII 117 GCTTAGC GC^T[ACGT]AGC
CfoI 115 GCGC GCG^C
DsaI 33 CCGCGG C^C[GA][CT]GG
EarI 302 GAAGAG CTCTTC(1/4)
Eco47III 114 AGCGCT AGC^GCT
FauI 35 GCGGG CCCGC(4/6)
HaeII 114 AGCGCT [GA]GCGC^[CT]
HhaI 115 GCGC GCG^C
HinP1I 115 GCGC G^CGC
HinfI 259 GAATC G^A[ACGT]TC
HphI 82 GGTGA GGTGA(8/7)
Ksp632I 302 GAAGAG CTCTTC(1/4)
KspI 33 CCGCGG CCGC^GG
MboII 302 GAAGA GAAGA(8/7)
MspA1I 33 CCGCGG C[AC]G^C[GT]G
MvnI 34 CGCG CG^CG
NspV 256 TTCGAA TT^CGAA
PpuMI 12 AGGTCCC [GA]G^G[AT]CC[CT]
RsaI 192 GTAC GT^AC
SacII 33 CCGCGG CCGC^GG
ScaI 191 AGTACT AGT^ACT
SfaNI 223 GATGC GCATC(5/9)
SfcI 110 CTACAG C^T[GA][CT]AG
SfuI 256 TTCGAA TT^CGAA
SspI 287 AATATT AAT^ATT
TaqI 257 TCGA T^CGA
TfiI 259 GAATC G^A[AT]TC
TseI 142 GCAGC G^C[AT]GC
TspRI 277 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 257 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 33 CCGC CCGC(-3/-1)
35 GCGG
AcsI 316 AAATTC [GA]^AATT[CT]
328 AAATTT
AluI 121 AGCT AG^CT
250 AGCT
ApaI 3 GGGCCC GGGCC^C
179 GGGCCC
ApoI 316 AAATTC [GA]^AATT[CT]
328 AAATTT
BanII 3 GGGCCC G[GA]GC[CT]^C
179 GGGCCC
BmyI 3 GGGCCC G[AGT]GC[ACT]^C
179 GGGCCC
BsiYI 28 CCGAGCCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
272 CCCAACAGTGG
BslI 28 CCGAGCCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
272 CCCAACAGTGG
Bsp1286I 3 GGGCCC G[AGT]GC[ACT]^C
179 GGGCCC
BstF5I 186 GGATG GGATG(2/0)
222 GGATG
DdeI 118 CTTAG C^T[ACGT]AG
216 CTTAG
DraII 12 AGGTCCC [GA]G^G[ACGT]CC[CT]
178 GGGGCCC
EcoO109I 12 AGGTCCC [GA]G^G[ACGT]CC[CT]
178 GGGGCCC
Fnu4HI 32 GCCGC GC^[ACGT]GC
142 GCAGC
FokI 186 GGATG GGATG(9/13)
222 GGATG
HapII 7 CCGG C^CGG
183 CCGG
HpaII 7 CCGG C^CGG
183 CCGG
ItaI 32 GCCGC GC^[ACGT]GC
142 GCAGC
MseI 244 TTAA T^TAA
313 TTAA
MspI 7 CCGG C^CGG
183 CCGG
SmaI 6 CCCGGG CCC^GGG
182 CCCGGG
Tru9I 244 TTAA T^TAA
313 TTAA
XmaCI 6 CCCGGG C^CCGGG
182 CCCGGG
XmaI 6 CCCGGG C^CCGGG
182 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AvaI BcnI BsaJI BsmFI BsoBI
BssKI Cfr13I HaeIII MnlI NciI
NlaIV Sau96I ScrFI Tsp509I