Restriction map for
pFN21AE2579
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AvaI AvrII BalI BbeI BbvCI
BciVI BfrI BlnI BsaBI BseAI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstAPI BstZ17I ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FseI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI NarI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflMI PinAI PmeI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AatII 475 GACGTC GACGT^C
AccI 931 GTCTAC GT^[AC][GT]AC
AcsI 743 AAATTC [GA]^AATT[CT]
AfaI 624 GTAC GT^AC
AlwNI 1483 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1073 CCTCAGG CC^T[ACGT]AGG
ApoI 743 AAATTC [GA]^AATT[CT]
AspHI 1036 GAGCTC G[AT]GC[AT]^C
AspI 799 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 296 TGCGCA TGC^GCA
BamHI 323 GGATCC G^GATCC
BanI 559 GGCACC G^G[CT][GA]CC
BanII 1036 GAGCTC G[GA]GC[CT]^C
BcgI 299 GCACCCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1580 CTAG C^TAG
BglII 311 AGATCT A^GATCT
BlpI 114 GCTCAGC GC^T[ACGT]AGC
BpmI 109 CTCCAG CTGGAG(16/14)
Bpu1102I 114 GCTCAGC GC^T[ACGT]AGC
BsaI 1171 GAGACC GGTCTC(1/5)
BsaWI 192 ACCGGA [AT]^CCGG[AT]
BseRI 832 GAGGAG GAGGAG(10/8)
BsiEI 704 CGGCCG CG[GA][CT]^CG
BsiHKAI 1036 GAGCTC G[AT]GC[AT]^C
BsmBI 1197 CGTCTC CGTCTC(1/5)
BspHI 341 TCATGA T^CATGA
BsrFI 1210 GCCGGC [GA]^CCGG[CT]
BstBI 137 TTCGAA TT^CGAA
BstEII 198 GGTCACC G^GT[ACGT]ACC
BstPI 198 GGTCACC G^GT[ACGT]ACC
BstXI 503 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1073 CCTCAGG CC^T[ACGT]AGG
CelII 114 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1210 GCCGGC [GA]^CCGG[CT]
DraIII 588 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 704 CGGCCG C^GGCCG
EclXI 704 CGGCCG C^GGCCG
Eco52I 704 CGGCCG C^GGCCG
Eco81I 1073 CCTCAGG CC^T[ACGT]AGG
EcoO65I 198 GGTCACC G^GT[ACGT]ACC
FspI 296 TGCGCA TGC^GCA
HaeII 1062 GGCGCT [GA]GCGC^[CT]
MaeI 1580 CTAG C^TAG
MseI 221 TTAA T^TAA
NaeI 1210 GCCGGC GCC^GGC
NcoI 102 CCATGG C^CATGG
NgoMIV 1210 GCCGGC G^CCGGC
NspV 137 TTCGAA TT^CGAA
PflFI 799 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 784 GAGTC GAGTC(4/5)
PpuMI 1520 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1233 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 466 CTGCAG CTGCA^G
RcaI 341 TCATGA T^CATGA
RsaI 624 GTAC GT^AC
SacI 1036 GAGCTC GAGCT^C
SexAI 1297 ACCTGGT A^CC[AT]GGT
SfuI 137 TTCGAA TT^CGAA
SmlI 375 CTCAAG C^T[CT][GA]AG
StuI 260 AGGCCT AGG^CCT
TfiI 1576 GATTC G^A[AT]TC
Tru9I 221 TTAA T^TAA
Tsp509I 744 AATT ^AATT
Tth111I 799 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 254 CGCG CG^CG
1273 CGCG
AflIII 291 ACACGT A^C[GA][CT]GT
852 ACACGT
Asp700I 597 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1413 GAAGCACTTC
BbrPI 292 CACGTG CAC^GTG
853 CACGTG
BbsI 478 GTCTTC GAAGAC(2/6)
1000 GTCTTC
BclI 237 TGATCA T^GATCA
434 TGATCA
BcnI 387 CCCGG CC^[GC]GG
978 CCGGG
BglI 531 GCCACTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
635 GCCTGGTGGGC
BpuAI 478 GTCTTC GAAGAC(2/6)
1000 GTCTTC
BsaAI 292 CACGTG [CT]AC^GT[GA]
853 CACGTG
BsgI 763 CTGCAC GTGCAG(16/14)
1480 GTGCAG
BspMI 680 ACCTGC ACCTGC(4/8)
1482 GCAGGT
BsrBI 185 CCGCTC CCGCTC(-3/-3)
991 CCGCTC
BssSI 155 CACGAG CACGAG(-5/-1)
58 CTCGTG
BstUI 254 CGCG CG^CG
1273 CGCG
EaeI 704 CGGCCG [CT]^GGCC[GA]
1208 TGGCCG
EcoNI 432 CCTGATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
825 CCTCTTTGAGG
FbaI 237 TGATCA T^GATCA
434 TGATCA
HinfI 784 GAGTC G^A[ACGT]TC
1576 GATTC
MslI 288 CACACACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
748 CACATCAATG
MvnI 254 CGCG CG^CG
1273 CGCG
NciI 387 CCCGG CC^[GC]GG
978 CCGGG
PmaCI 292 CACGTG CAC^GTG
853 CACGTG
PmlI 292 CACGTG CAC^GTG
853 CACGTG
SfcI 466 CTGCAG C^T[GA][CT]AG
1008 CTACAG
XmnI 597 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1413 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcyI AluI AlwI AvaII
BbvI BmyI BsaHI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
SapI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XhoII