Restriction map for
pFN21AE3878
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# NO CUT ENZYMES ( 102 enzymes) #
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Acc65I AclI AcsI AflII AgeI
AlwNI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BbrPI BbvCI BcgI BciVI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrBI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI HindIII HpaI KpnI
MaeI MamI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I RsrII SacI SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 1702 GACGTC GACGT^C
AccIII 1268 TCCGGA T^CCGGA
AvaII 186 GGTCC G^G[AT]CC
BamHI 71 GGATCC G^GATCC
BbsI 352 GAAGAC GAAGAC(2/6)
BclI 713 TGATCA T^GATCA
BglII 872 AGATCT A^GATCT
BpuAI 352 GAAGAC GAAGAC(2/6)
BsaI 1609 GAGACC GGTCTC(1/5)
BseAI 1268 TCCGGA T^CCGGA
BsiEI 1715 CGATCG CG[GA][CT]^CG
BsmBI 1657 GAGACG CGTCTC(1/5)
BspEI 1268 TCCGGA T^CCGGA
BspHI 716 TCATGA T^CATGA
BsrDI 1101 CATTGC GCAATG(2/0)
BsrFI 1207 GCCGGT [GA]^CCGG[CT]
BssHII 41 GCGCGC G^CGCGC
BstAPI 326 GCACGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 782 CCATTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1207 GCCGGT [GA]^CCGG[CT]
DraI 693 TTTAAA TTT^AAA
DraIII 1669 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 713 TGATCA T^GATCA
HgaI 522 GACGC GACGC(5/10)
HincII 931 GTTGAC GT[CT]^[GA]AC
HindII 931 GTTGAC GT[CT]^[GA]AC
KspI 77 CCGCGG CCGC^GG
MroI 1268 TCCGGA T^CCGGA
NspI 1749 GCATGT [GA]CATG^[CT]
PacI 865 TTAATTAA TTAAT^TAA
PleI 133 GACTC GAGTC(4/5)
PpuMI 185 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 1636 CTGCAG CTGCA^G
PvuI 1715 CGATCG CGAT^CG
RcaI 716 TCATGA T^CATGA
SacII 77 CCGCGG CCGC^GG
ScaI 1226 AGTACT AGT^ACT
SmaI 1413 CCCGGG CCC^GGG
StuI 1378 AGGCCT AGG^CCT
XmaCI 1413 CCCGGG C^CCGGG
XmaI 1413 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1015 GTCTAC GT^[AC][GT]AC
1588 GTCTAC
AflIII 1509 ACACGT A^C[GA][CT]GT
1660 ACGTGT
AhdI 1611 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1659 GACGTGTCGTC
Alw44I 112 GTGCAC G^TGCAC
1154 GTGCAC
ApaI 123 GGGCCC GGGCC^C
971 GGGCCC
ApaLI 112 GTGCAC G^TGCAC
1154 GTGCAC
AspEI 1611 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1659 GACGTGTCGTC
AviII 925 TGCGCA TGC^GCA
1083 TGCGCA
BbeI 25 GGCGCC GGCGC^C
82 GGCGCC
BsaWI 281 ACCGGA [AT]^CCGG[AT]
1268 TCCGGA
BsgI 1881 CTGCAC GTGCAG(16/14)
1696 GTGCAG
BstYI 71 GGATCC [GA]^GATC[CT]
872 AGATCT
Eam1105I 1611 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1659 GACGTGTCGTC
EarI 541 GAAGAG CTCTTC(1/4)
1519 GAAGAG
FspI 925 TGCGCA TGC^GCA
1083 TGCGCA
KasI 25 GGCGCC G^GCGCC
82 GGCGCC
Ksp632I 541 GAAGAG CTCTTC(1/4)
1519 GAAGAG
MflI 71 GGATCC [GA]^GATC[CT]
872 AGATCT
NarI 25 GGCGCC GG^CGCC
82 GGCGCC
PaeR7I 308 CTCGAG C^TCGAG
1042 CTCGAG
SfcI 1636 CTGCAG C^T[GA][CT]AG
1734 CTACAG
SfoI 25 GGCGCC GGC^GCC
82 GGCGCC
SmlI 308 CTCGAG C^T[CT][GA]AG
1042 CTCGAG
Tsp509I 867 AATT ^AATT
1534 AATT
XcmI 779 CCACCATTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1792 CCAGTTCTCCGCTGG
XhoI 308 CTCGAG C^TCGAG
1042 CTCGAG
XhoII 71 GGATCC [GA]^GATC[CT]
872 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI BalI BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I