Restriction map for
pFN21AE5677
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccII AcyI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BfrI BglII BlnI
BpmI BpuAI BsaHI BsaI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspMI BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FauI FseI FspI
Hin1I HindIII KasI KpnI KspI
MfeI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 71 TCCGGA T^CCGGA
AclI 1387 AACGTT AA^CGTT
AcsI 1207 AAATTT [GA]^AATT[CT]
AfaI 268 GTAC GT^AC
AflIII 215 ACATGT A^C[GA][CT]GT
AlwNI 1636 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 723 AGCGCT AGC^GCT
ApoI 1207 AAATTT [GA]^AATT[CT]
AseI 655 ATTAAT AT^TAAT
AsnI 655 ATTAAT AT^TAAT
BalI 479 TGGCCA TGG^CCA
BanII 191 GAGCTC G[GA]GC[CT]^C
BbrPI 454 CACGTG CAC^GTG
BclI 374 TGATCA T^GATCA
BfaI 1543 CTAG C^TAG
BlpI 387 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 387 GCTGAGC GC^T[ACGT]AGC
BsaAI 454 CACGTG [CT]AC^GT[GA]
BsaWI 71 TCCGGA [AT]^CCGG[AT]
BseAI 71 TCCGGA T^CCGGA
BsiEI 408 CGACCG CG[GA][CT]^CG
BspEI 71 TCCGGA T^CCGGA
BspHI 1050 TCATGA T^CATGA
BspLU11I 215 ACATGT A^CATGT
BsrDI 569 CATTGC GCAATG(2/0)
BsrFI 518 ACCGGC [GA]^CCGG[CT]
BssSI 194 CTCGTG CACGAG(-5/-1)
BstYI 68 AGATCC [GA]^GATC[CT]
CelII 387 GCTGAGC GC^T[ACGT]AGC
Cfr10I 518 ACCGGC [GA]^CCGG[CT]
DraII 1315 AGGACCC [GA]G^G[ACGT]CC[CT]
Eco47III 723 AGCGCT AGC^GCT
EcoO109I 1315 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 500 ATGCAT ATGCA^T
FbaI 374 TGATCA T^GATCA
HaeII 723 AGCGCT [GA]GCGC^[CT]
HgaI 1412 GACGC GACGC(5/10)
MaeI 1543 CTAG C^TAG
MflI 68 AGATCC [GA]^GATC[CT]
MluNI 479 TGGCCA TGG^CCA
MroI 71 TCCGGA T^CCGGA
MscI 479 TGGCCA TGG^CCA
MslI 553 CACTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 500 ATGCAT ATGCA^T
PleI 225 GACTC GAGTC(4/5)
PmaCI 454 CACGTG CAC^GTG
PmlI 454 CACGTG CAC^GTG
PpuMI 1315 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 936 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 655 ATTAAT AT^TAAT
Psp1406I 1387 AACGTT AA^CGTT
RcaI 1050 TCATGA T^CATGA
RsaI 268 GTAC GT^AC
SacI 191 GAGCTC GAGCT^C
SphI 630 GCATGC GCATG^C
XbaI 1542 TCTAGA T^CTAGA
XhoII 68 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 58 GATCC GGATC(4/5)
69 GATCC
AvaII 516 GGACC G^G[AT]CC
1316 GGACC
BcnI 237 CCGGG CC^[GC]GG
1581 CCGGG
BglI 346 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
481 GCCAAAGGGGC
BsaBI 75 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1564 GATGCAAATC
BseRI 1022 CTCCTC GAGGAG(10/8)
64 GAGGAG
BsmAI 1363 GAGAC GTCTC(1/5)
403 GTCTC
BstNI 681 CCTGG CC^[AT]GG
1508 CCAGG
DsaI 582 CCATGG C^C[GA][CT]GG
945 CCATGG
EarI 131 CTCTTC CTCTTC(1/4)
329 CTCTTC
EcoRII 681 CCTGG ^CC[AT]GG
1508 CCAGG
HincII 763 GTTAAC GT[CT]^[GA]AC
895 GTTAAC
HindII 763 GTTAAC GT[CT]^[GA]AC
895 GTTAAC
HpaI 763 GTTAAC GTT^AAC
895 GTTAAC
Ksp632I 131 CTCTTC CTCTTC(1/4)
329 CTCTTC
MamI 75 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1564 GATGCAAATC
MvaI 681 CCTGG CC^[AT]GG
1508 CCAGG
NciI 237 CCGGG CC^[GC]GG
1581 CCGGG
NcoI 582 CCATGG C^CATGG
945 CCATGG
NlaIV 1316 GGACCC GG[ACGT]^[ACGT]CC
1381 GGGGCC
TfiI 112 GATTC G^A[AT]TC
1106 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AspHI BbvI BmyI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MwoI NdeII NlaIII NspI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI Tru9I TseI Tsp45I
Tsp509I TspRI