Restriction map for
pFN21AE2519
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AlwI AocI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BciVI BclI BfaI BlnI
BlpI Bpu1102I BsaAI BsaI BsgI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MaeI
MfeI MluI MluNI MscI MunI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 1531 GTCTAC GT^[AC][GT]AC
AccIII 1106 TCCGGA T^CCGGA
AflII 1654 CTTAAG C^TTAAG
AflIII 206 ACGTGT A^C[GA][CT]GT
Alw44I 1246 GTGCAC G^TGCAC
AlwNI 1202 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1246 GTGCAC G^TGCAC
Asp700I 1098 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 414 GGTGCC G^G[CT][GA]CC
BanII 564 GGGCTC G[GA]GC[CT]^C
BbvCI 1476 GCTGAGG CCTCAGC(-5/-2)
BfrI 1654 CTTAAG C^TTAAG
BglI 1282 GCCCCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1049 AGATCT A^GATCT
BsaBI 1044 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1106 TCCGGA [AT]^CCGG[AT]
BseAI 1106 TCCGGA T^CCGGA
BspEI 1106 TCCGGA T^CCGGA
BspHI 185 TCATGA T^CATGA
BsrBI 1093 GAGCGG CCGCTC(-3/-3)
BsrDI 1178 GCAATG GCAATG(2/0)
BsrFI 328 GCCGGC [GA]^CCGG[CT]
BstXI 314 CCAGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1049 AGATCT [GA]^GATC[CT]
Cfr10I 328 GCCGGC [GA]^CCGG[CT]
EaeI 1198 CGGCCA [CT]^GGCC[GA]
EcoRV 487 GATATC GAT^ATC
FauI 795 GCGGG CCCGC(4/6)
HincII 1561 GTCAAC GT[CT]^[GA]AC
HindII 1561 GTCAAC GT[CT]^[GA]AC
MamI 1044 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1049 AGATCT [GA]^GATC[CT]
MroI 1106 TCCGGA T^CCGGA
MseI 1655 TTAA T^TAA
NaeI 328 GCCGGC GCC^GGC
NgoMIV 328 GCCGGC G^CCGGC
PvuII 745 CAGCTG CAG^CTG
RcaI 185 TCATGA T^CATGA
SapI 477 GCTCTTC GCTCTTC(1/4)
SbfI 304 CCTGCAGG CCTGCA^GG
Sse8387I 304 CCTGCAGG CCTGCA^GG
StuI 669 AGGCCT AGG^CCT
TfiI 1553 GAATC G^A[AT]TC
Tru9I 1655 TTAA T^TAA
XhoII 1049 AGATCT [GA]^GATC[CT]
XmnI 1098 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 1084 CGCG CG^CG
1443 CGCG
AcsI 190 AAATTC [GA]^AATT[CT]
1640 AAATTC
AcyI 1144 GGCGTC G[GA]^CG[CT]C
1218 GACGCC
Aor51HI 428 AGCGCT AGC^GCT
1265 AGCGCT
ApoI 190 AAATTC [GA]^AATT[CT]
1640 AAATTC
BbsI 1455 GAAGAC GAAGAC(2/6)
715 GTCTTC
BcgI 1279 GCAGCCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1595 GCAACCACGTCG
BcnI 504 CCCGG CC^[GC]GG
773 CCCGG
BpuAI 1455 GAAGAC GAAGAC(2/6)
715 GTCTTC
BsaHI 1144 GGCGTC G[GA]^CG[CT]C
1218 GACGCC
BstUI 1084 CGCG CG^CG
1443 CGCG
DsaI 132 CCATGG C^C[GA][CT]GG
605 CCATGG
Eco47III 428 AGCGCT AGC^GCT
1265 AGCGCT
Hin1I 1144 GGCGTC G[GA]^CG[CT]C
1218 GACGCC
MslI 360 CACTGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
801 CATTGACGTG
MspA1I 745 CAGCTG C[AC]G^C[GT]G
793 CAGCGG
MvnI 1084 CGCG CG^CG
1443 CGCG
NciI 504 CCCGG CC^[GC]GG
773 CCCGG
NcoI 132 CCATGG C^CATGG
605 CCATGG
PflMI 132 CCATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
655 CCAGCAGGTGG
SfaNI 474 GATGC GCATC(5/9)
1080 GATGC
TaqI 472 TCGA T^CGA
719 TCGA
Tsp509I 191 AATT ^AATT
1641 AATT
TthHB8I 472 TCGA T^CGA
719 TCGA
Van91I 132 CCATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
655 CCAGCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BbvI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI StyI TseI Tsp45I TspRI