Restriction map for
pFN21AE2562
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwI AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI BspDI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MscI MspA1I
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccIII 740 TCCGGA T^CCGGA
AciI 423 GCGG CCGC(-3/-1)
AcsI 712 AAATTC [GA]^AATT[CT]
AfaI 247 GTAC GT^AC
AocI 33 CCTGAGG CC^T[ACGT]AGG
ApoI 712 AAATTC [GA]^AATT[CT]
Asp700I 732 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 115 GAGCAC G[AT]GC[AT]^C
AvaII 631 GGACC G^G[AT]CC
BanII 27 GAGCCC G[GA]GC[CT]^C
BbsI 646 GAAGAC GAAGAC(2/6)
BbvI 475 GCTGC GCAGC(8/12)
BfaI 635 CTAG C^TAG
BglII 768 AGATCT A^GATCT
BpmI 434 CTGGAG CTGGAG(16/14)
BpuAI 646 GAAGAC GAAGAC(2/6)
BsaWI 740 TCCGGA [AT]^CCGG[AT]
BseAI 740 TCCGGA T^CCGGA
BsiHKAI 115 GAGCAC G[AT]GC[AT]^C
BsiYI 433 CCTGGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 433 CCTGGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 789 GTCTC GTCTC(1/5)
Bsp1407I 246 TGTACA T^GTACA
BspEI 740 TCCGGA T^CCGGA
BsrDI 190 GCAATG GCAATG(2/0)
BsrGI 246 TGTACA T^GTACA
BssKI 433 CCTGG ^CC[ACGT]GG
BstNI 433 CCTGG CC^[AT]GG
BstYI 768 AGATCT [GA]^GATC[CT]
Bsu36I 33 CCTGAGG CC^T[ACGT]AGG
Cac8I 666 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 568 GCGC GCG^C
DpnII 769 GATC ^GATC
Eco81I 33 CCTGAGG CC^T[ACGT]AGG
EcoRII 433 CCTGG ^CC[AT]GG
EcoT14I 264 CCTTGG C^C[AT][AT]GG
Fnu4HI 475 GCTGC GC^[ACGT]GC
HaeII 567 AGCGCC [GA]GCGC^[CT]
HapII 741 CCGG C^CGG
HhaI 568 GCGC GCG^C
HinP1I 568 GCGC G^CGC
HpaII 741 CCGG C^CGG
ItaI 475 GCTGC GC^[ACGT]GC
MaeI 635 CTAG C^TAG
MaeIII 220 GTAAC ^GT[ACGT]AC
MboI 769 GATC ^GATC
MflI 768 AGATCT [GA]^GATC[CT]
MroI 740 TCCGGA T^CCGGA
MspI 741 CCGG C^CGG
MvaI 433 CCTGG CC^[AT]GG
NdeII 769 GATC ^GATC
PleI 370 GAGTC GAGTC(4/5)
PpuMI 630 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 247 GTAC GT^AC
Sau3AI 769 GATC ^GATC
ScrFI 433 CCTGG CC^[ACGT]GG
SfaNI 364 GATGC GCATC(5/9)
SfcI 641 CTGTAG C^T[GA][CT]AG
SspBI 246 TGTACA T^GTACA
StyI 264 CCTTGG C^C[AT][AT]GG
TaqI 10 TCGA T^CGA
TseI 475 GCTGC G^C[AT]GC
TthHB8I 10 TCGA T^CGA
XhoII 768 AGATCT [GA]^GATC[CT]
XmnI 732 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AflII 297 CTTAAG C^TTAAG
405 CTTAAG
AluI 351 AGCT AG^CT
555 AGCT
BfrI 297 CTTAAG C^TTAAG
405 CTTAAG
BmyI 27 GAGCCC G[AGT]GC[ACT]^C
115 GAGCAC
BsaJI 264 CCTTGG C^C[ACGT][ACGT]GG
432 CCCTGG
BseRI 385 GAGGAG GAGGAG(10/8)
388 GAGGAG
Bsp1286I 27 GAGCCC G[AGT]GC[ACT]^C
115 GAGCAC
DdeI 34 CTGAG C^T[ACGT]AG
345 CTTAG
DraII 37 AGGCCCC [GA]G^G[ACGT]CC[CT]
630 AGGACCT
EcoO109I 37 AGGCCCC [GA]G^G[ACGT]CC[CT]
630 AGGACCT
MslI 18 CATGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
328 CATATTAGTG
NlaIII 18 CATG CATG^
24 CATG
NlaIV 38 GGCCCC GG[ACGT]^[ACGT]CC
450 GGTTCC
SmlI 297 CTTAAG C^T[CT][GA]AG
405 CTTAAG
TfiI 7 GATTC G^A[AT]TC
589 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
BsrI BstF5I Cfr13I EarI FokI
HaeIII HinfI Ksp632I MboII MnlI
MseI Sau96I Tru9I Tsp509I TspRI