##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaII       AviII       AvrII       BamHI       
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BpuAI       BsaBI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DpnII       DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HinfI       HpaI        
KpnI        KspI        MaeI        MaeIII      MamI        
MboI        MfeI        MflI        MluI        MroI        
MseI        MunI        MvnI        NaeI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StyI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
Tth111I     TthHB8I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

ApaI          409  GGGCCC               GGGCC^C
BanI          193  GGCGCC               G^G[CT][GA]CC
BbeI          193  GGCGCC               GGCGC^C
BcnI          523  CCCGG                CC^[GC]GG
BlpI          630  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      630  GCTGAGC              GC^T[ACGT]AGC
BsaAI         415  TACGTG               [CT]AC^GT[GA]
BstAPI        353  GCACCCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        333  GGATG                GGATG(2/0)
BstXI         269  CCAGTGGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         630  GCTGAGC              GC^T[ACGT]AGC
DsaI          105  CCACGG               C^C[GA][CT]GG
EarI           27  GAAGAG               CTCTTC(1/4)
FokI          333  GGATG                GGATG(9/13)
HapII         524  CCGG                 C^CGG
HgaI          553  GACGC                GACGC(5/10)
HpaII         524  CCGG                 C^CGG
HphI          429  GGTGA                GGTGA(8/7)
KasI          193  GGCGCC               G^GCGCC
Ksp632I        27  GAAGAG               CTCTTC(1/4)
MboII          27  GAAGA                GAAGA(8/7)
MspI          524  CCGG                 C^CGG
NarI          193  GGCGCC               GG^CGCC
NciI          523  CCCGG                CC^[GC]GG
PflMI         105  CCACGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI           54  CTGCAG               CTGCA^G
PvuII         537  CAGCTG               CAG^CTG
SexAI         425  ACCAGGT              A^CC[AT]GGT
SfoI          193  GGCGCC               GGC^GCC
StuI           74  AGGCCT               AGG^CCT
Van91I        105  CCACGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          389  CCACAGCAGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AcyI          193  GGCGCC               G[GA]^CG[CT]C
              553  GACGCC              
BanII         409  GGGCCC               G[GA]GC[CT]^C
              613  GAGCCC              
BpmI          348  CTGGAG               CTGGAG(16/14)
              400  CTGGAG              
BsaHI         193  GGCGCC               G[GA]^CG[CT]C
              553  GACGCC              
CfoI          194  GCGC                 GCG^C
              345  GCGC                
DdeI           10  CTCAG                C^T[ACGT]AG
              631  CTGAG               
HaeII         193  GGCGCC               [GA]GCGC^[CT]
              344  GGCGCT              
HhaI          194  GCGC                 GCG^C
              345  GCGC                
Hin1I         193  GGCGCC               G[GA]^CG[CT]C
              553  GACGCC              
HinP1I        194  GCGC                 G^CGC
              345  GCGC                
MaeII         329  ACGT                 A^CGT
              416  ACGT                
MslI          228  CATGACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              315  CATGCGGGTG          


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AciI        AluI        AspHI       AvaI        BalI        
BbvI        BmyI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstNI       Cac8I       Cfr13I      DraII       
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
HaeIII      ItaI        MluNI       MnlI        MscI        
MspA1I      MvaI        MwoI        NlaIII      NlaIV       
Sau96I      ScrFI       SfaNI       SfcI        TseI        
TspRI