Restriction map for
pFN21AE6389
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII AccII AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseRI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HindIII HpaI KasI
KspI MaeII MaeIII MamI MluI
MseI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tsp45I Tth111I TthHB8I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 1129 GGTACC G^GTACC
AccIII 5 TCCGGA T^CCGGA
AciI 30 GCGG CCGC(-3/-1)
AlwI 607 GGATC GGATC(4/5)
AlwNI 338 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 471 GGGCCC GGGCC^C
Asp718I 1129 GGTACC G^GTACC
AvaI 410 CCCGGG C^[CT]CG[GA]G
BglI 790 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 5 TCCGGA [AT]^CCGG[AT]
BseAI 5 TCCGGA T^CCGGA
BsmAI 847 GTCTC GTCTC(1/5)
BsmI 638 GAATGC GAATGC(1/-1)
BsoBI 410 CCCGGG C^[CT]CG[GA]G
BspEI 5 TCCGGA T^CCGGA
BspMI 628 GCAGGT ACCTGC(4/8)
BstAPI 60 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1079 CCAACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 607 GGATCT [GA]^GATC[CT]
DpnII 608 GATC ^GATC
EcoRV 494 GATATC GAT^ATC
HgaI 52 GCGTC GACGC(5/10)
HinfI 870 GACTC G^A[ACGT]TC
HphI 479 TCACC GGTGA(8/7)
KpnI 1129 GGTACC GGTAC^C
MboI 608 GATC ^GATC
MfeI 128 CAATTG C^AATTG
MflI 607 GGATCT [GA]^GATC[CT]
MroI 5 TCCGGA T^CCGGA
MunI 128 CAATTG C^AATTG
NcoI 683 CCATGG C^CATGG
NdeII 608 GATC ^GATC
PleI 870 GACTC GAGTC(4/5)
PpuMI 562 GGGACCT [GA]G^G[AT]CC[CT]
PstI 914 CTGCAG CTGCA^G
SacI 728 GAGCTC GAGCT^C
Sau3AI 608 GATC ^GATC
SmaI 410 CCCGGG CCC^GGG
Tsp509I 129 AATT ^AATT
XhoII 607 GGATCT [GA]^GATC[CT]
XmaCI 410 CCCGGG C^CCGGG
XmaI 410 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccI 652 GTCTAC GT^[AC][GT]AC
1145 GTCTAC
BfaI 290 CTAG C^TAG
1018 CTAG
BsgI 181 CTGCAC GTGCAG(16/14)
770 CTGCAC
DdeI 354 CTCAG C^T[ACGT]AG
854 CTGAG
DsaI 266 CCGTGG C^C[GA][CT]GG
683 CCATGG
EarI 174 CTCTTC CTCTTC(1/4)
400 GAAGAG
HincII 169 GTCAAC GT[CT]^[GA]AC
457 GTCAAC
HindII 169 GTCAAC GT[CT]^[GA]AC
457 GTCAAC
Ksp632I 174 CTCTTC CTCTTC(1/4)
400 GAAGAG
MaeI 290 CTAG C^TAG
1018 CTAG
NspI 104 ACATGC [GA]CATG^[CT]
210 ACATGC
PvuII 61 CAGCTG CAG^CTG
1009 CAGCTG
SfcI 832 CTACAG C^T[GA][CT]AG
914 CTGCAG
XcmI 935 CCAACATGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1001 CCAAGTGGCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AfaI AluI AspHI AvaII BalI
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HpaII ItaI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
StyI TseI TspRI