Restriction map for
pFN21AE5277
##################################
# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CpoI DraI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MluI
MroI MseI MslI MspA1I MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I TspRI
Tth111I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AfaI 627 GTAC GT^AC
AspHI 325 GTGCTC G[AT]GC[AT]^C
AvaI 108 CCCGGG C^[CT]CG[GA]G
BalI 584 TGGCCA TGG^CCA
BanI 407 GGCACC G^G[CT][GA]CC
BanII 439 GAGCCC G[GA]GC[CT]^C
BbvCI 333 CCTCAGC CCTCAGC(-5/-2)
BfaI 436 CTAG C^TAG
BglI 416 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 567 CTCCAG CTGGAG(16/14)
BsiHKAI 325 GTGCTC G[AT]GC[AT]^C
BsoBI 108 CCCGGG C^[CT]CG[GA]G
BspDI 16 ATCGAT AT^CGAT
BsrBI 221 GAGCGG CCGCTC(-3/-3)
BsrFI 404 ACCGGC [GA]^CCGG[CT]
BsrI 362 ACTGG ACTGG(1/-1)
BstBI 143 TTCGAA TT^CGAA
BstYI 398 GGATCT [GA]^GATC[CT]
CfoI 384 GCGC GCG^C
Cfr10I 404 ACCGGC [GA]^CCGG[CT]
ClaI 16 ATCGAT AT^CGAT
DraIII 342 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 226 GACACATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 584 TGGCCA [CT]^GGCC[GA]
EcoRV 13 GATATC GAT^ATC
EcoT14I 212 CCTTGG C^C[AT][AT]GG
FauI 41 CCCGC CCCGC(4/6)
HhaI 384 GCGC GCG^C
HinP1I 384 GCGC G^CGC
MaeI 436 CTAG C^TAG
MaeII 557 ACGT A^CGT
MaeIII 259 GTCAC ^GT[ACGT]AC
MfeI 514 CAATTG C^AATTG
MflI 398 GGATCT [GA]^GATC[CT]
MluNI 584 TGGCCA TGG^CCA
MscI 584 TGGCCA TGG^CCA
MunI 514 CAATTG C^AATTG
NlaIII 387 CATG CATG^
NspI 386 GCATGT [GA]CATG^[CT]
NspV 143 TTCGAA TT^CGAA
PflMI 90 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 474 GACTC GAGTC(4/5)
RsaI 627 GTAC GT^AC
SexAI 290 ACCTGGT A^CC[AT]GGT
SfuI 143 TTCGAA TT^CGAA
SmaI 108 CCCGGG CCC^GGG
StyI 212 CCTTGG C^C[AT][AT]GG
Tsp45I 259 GTCAC ^GT[GC]AC
Van91I 90 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 398 GGATCT [GA]^GATC[CT]
XmaCI 108 CCCGGG C^CCGGG
XmaI 108 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 42 CCGC CCGC(-3/-1)
223 GCGG
BbsI 235 GTCTTC GAAGAC(2/6)
253 GTCTTC
BpuAI 235 GTCTTC GAAGAC(2/6)
253 GTCTTC
BseRI 70 GAGGAG GAGGAG(10/8)
150 GAGGAG
BsmAI 368 GAGAC GTCTC(1/5)
80 GTCTC
BsmBI 79 CGTCTC CGTCTC(1/5)
368 GAGACG
Cac8I 40 GCCCGC GC[ACGT]^[ACGT]GC
178 GCCAGC
DdeI 311 CTGAG C^T[ACGT]AG
334 CTCAG
EcoNI 63 CCTTTCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
291 CCTGGTGGAGG
HgaI 78 GCGTC GACGC(5/10)
618 GCGTC
HphI 121 GGTGA GGTGA(8/7)
341 TCACC
MboII 236 TCTTC GAAGA(8/7)
254 TCTTC
PpuMI 104 GGGACCC [GA]G^G[AT]CC[CT]
186 GGGTCCC
SfaNI 354 GCATC GCATC(5/9)
425 GCATC
TfiI 19 GATTC G^A[AT]TC
509 GAATC
Tsp509I 268 AATT ^AATT
515 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwI AvaII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI Cfr13I
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MboI MnlI MspI MvaI
MwoI NciI NdeII NlaIV Sau3AI
Sau96I ScrFI TaqI TseI TthHB8I