Restriction map for
pFN21AE2574
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I CelII ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HpaI KasI KpnI
Ksp632I MaeII MamI MflI MluI
MluNI MroI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI SexAI SfcI
SfiI SfoI SfuI SmlI SnaBI
SpeI SphI Sse8387I SwaI TfiI
Tth111I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 769 ACATGT A^C[GA][CT]GT
AocI 489 CCTGAGG CC^T[ACGT]AGG
Aor51HI 9 AGCGCT AGC^GCT
ApaI 41 GGGCCC GGGCC^C
Asp700I 430 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 194 GAGCAC G[AT]GC[AT]^C
AvaI 483 CCCGGG C^[CT]CG[GA]G
AvrII 930 CCTAGG C^CTAGG
BanII 41 GGGCCC G[GA]GC[CT]^C
BlnI 930 CCTAGG C^CTAGG
BpmI 469 CTCCAG CTGGAG(16/14)
BsaWI 903 TCCGGT [AT]^CCGG[AT]
BseRI 355 CTCCTC GAGGAG(10/8)
BsiHKAI 194 GAGCAC G[AT]GC[AT]^C
BsmAI 318 GTCTC GTCTC(1/5)
BsmI 704 GAATGC GAATGC(1/-1)
BsoBI 483 CCCGGG C^[CT]CG[GA]G
Bsp1407I 339 TGTACA T^GTACA
BspLU11I 769 ACATGT A^CATGT
BsrGI 339 TGTACA T^GTACA
BstAPI 985 GCAGTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 489 CCTGAGG CC^T[ACGT]AGG
DsaI 45 CCGCGG C^C[GA][CT]GG
Eco47III 9 AGCGCT AGC^GCT
Eco81I 489 CCTGAGG CC^T[ACGT]AGG
EcoNI 925 CCTTTCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 88 GCGTC GACGC(5/10)
HindIII 406 AAGCTT A^AGCTT
HinfI 316 GAGTC G^A[ACGT]TC
HphI 261 TCACC GGTGA(8/7)
KspI 45 CCGCGG CCGC^GG
MfeI 716 CAATTG C^AATTG
MunI 716 CAATTG C^AATTG
PflMI 562 CCACATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 316 GAGTC GAGTC(4/5)
SacII 45 CCGCGG CCGC^GG
ScaI 365 AGTACT AGT^ACT
SfaNI 1081 GCATC GCATC(5/9)
SgrAI 57 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 483 CCCGGG CCC^GGG
SspBI 339 TGTACA T^GTACA
SspI 895 AATATT AAT^ATT
StuI 465 AGGCCT AGG^CCT
Tsp45I 766 GTGAC ^GT[GC]AC
Van91I 562 CCACATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 483 CCCGGG C^CCGGG
XmaI 483 CCCGGG C^CCGGG
XmnI 430 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 46 CGCG CG^CG
87 CGCG
AlwI 498 GATCC GGATC(4/5)
823 GATCC
AvaII 906 GGTCC G^G[AT]CC
1213 GGACC
BanI 3 GGTGCC G^G[CT][GA]CC
28 GGTGCC
BfaI 931 CTAG C^TAG
973 CTAG
BmyI 41 GGGCCC G[AGT]GC[ACT]^C
194 GAGCAC
BsmFI 481 GTCCC GGGAC(10/14)
907 GTCCC
Bsp1286I 41 GGGCCC G[AGT]GC[ACT]^C
194 GAGCAC
BsrFI 58 GCCGGT [GA]^CCGG[CT]
917 GCCGGT
BstNI 70 CCTGG CC^[AT]GG
609 CCAGG
BstUI 46 CGCG CG^CG
87 CGCG
CfoI 10 GCGC GCG^C
128 GCGC
Cfr10I 58 GCCGGT [GA]^CCGG[CT]
917 GCCGGT
DpnII 498 GATC ^GATC
823 GATC
DraI 997 TTTAAA TTT^AAA
1318 TTTAAA
DraII 449 AGGCCCT [GA]G^G[ACGT]CC[CT]
975 AGGGCCT
EcoO109I 449 AGGCCCT [GA]G^G[ACGT]CC[CT]
975 AGGGCCT
EcoRII 70 CCTGG ^CC[AT]GG
609 CCAGG
EcoT14I 930 CCTAGG C^C[AT][AT]GG
949 CCAAGG
HaeII 9 AGCGCT [GA]GCGC^[CT]
127 AGCGCC
HhaI 10 GCGC GCG^C
128 GCGC
HinP1I 10 GCGC G^CGC
128 GCGC
MaeI 931 CTAG C^TAG
973 CTAG
MboI 498 GATC ^GATC
823 GATC
MboII 430 GAAGA GAAGA(8/7)
1152 TCTTC
MslI 274 CATAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1239 CATGAAAGTG
MspA1I 45 CCGCGG C[AC]G^C[GT]G
106 CCGCTG
MvaI 70 CCTGG CC^[AT]GG
609 CCAGG
MvnI 46 CGCG CG^CG
87 CGCG
NdeII 498 GATC ^GATC
823 GATC
NspI 769 ACATGT [GA]CATG^[CT]
1123 GCATGT
Sau3AI 498 GATC ^GATC
823 GATC
StyI 930 CCTAGG C^C[AT][AT]GG
949 CCAAGG
TaqI 750 TCGA T^CGA
828 TCGA
TthHB8I 750 TCGA T^CGA
828 TCGA
XcmI 618 CCAAGAAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1034 CCACAGATGTTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BcnI BsaJI BsgI BsiYI
BslI BspMI BsrI BssKI BstF5I
Cac8I Cfr13I DdeI FauI Fnu4HI
FokI HaeIII HapII HpaII ItaI
MaeIII MnlI MseI MspI MwoI
NciI NlaIII NlaIV RsaI Sau96I
ScrFI Tru9I TseI Tsp509I TspRI