Restriction map for
pFN21AB4189
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI Bsp1407I
BspDI BspEI BspMI BsrGI BssHII
BssSI BstAPI BstEII BstPI BstUI
BstXI CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NarI NcoI NheI NotI NruI
NsiI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tth111I Van91I XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 403 GTATAC GT^[AC][GT]AC
AfaI 63 GTAC GT^AC
AlwNI 1154 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2206 GGGCCC GGGCC^C
AspHI 1210 GAGCAC G[AT]GC[AT]^C
BamHI 960 GGATCC G^GATCC
BanII 2206 GGGCCC G[GA]GC[CT]^C
BciVI 1893 GGATAC GTATCC(6/5)
BpmI 422 CTCCAG CTGGAG(16/14)
BsaAI 406 TACGTG [CT]AC^GT[GA]
BsiHKAI 1210 GAGCAC G[AT]GC[AT]^C
BspHI 1749 TCATGA T^CATGA
BspLU11I 633 ACATGT A^CATGT
BsrBI 3 GAGCGG CCGCTC(-3/-3)
BsrDI 201 CATTGC GCAATG(2/0)
BsrFI 100 GCCGGC [GA]^CCGG[CT]
Bst1107I 403 GTATAC GTA^TAC
BstZ17I 403 GTATAC GTA^TAC
Cfr10I 100 GCCGGC [GA]^CCGG[CT]
EaeI 1014 TGGCCG [CT]^GGCC[GA]
MaeII 407 ACGT A^CGT
MaeIII 83 GTGAC ^GT[ACGT]AC
MspA1I 1434 CAGCTG C[AC]G^C[GT]G
NaeI 100 GCCGGC GCC^GGC
NdeI 1898 CATATG CA^TATG
NgoMIV 100 GCCGGC G^CCGGC
NspI 633 ACATGT [GA]CATG^[CT]
PaeR7I 1708 CTCGAG C^TCGAG
PpuMI 155 GGGACCT [GA]G^G[AT]CC[CT]
PstI 287 CTGCAG CTGCA^G
PvuII 1434 CAGCTG CAG^CTG
RcaI 1749 TCATGA T^CATGA
RsaI 63 GTAC GT^AC
StuI 1140 AGGCCT AGG^CCT
Tsp45I 83 GTGAC ^GT[GC]AC
XbaI 1189 TCTAGA T^CTAGA
XcmI 1032 CCAGGCATGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1708 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 407 ACGTGT A^C[GA][CT]GT
633 ACATGT
AocI 1126 CCTGAGG CC^T[ACGT]AGG
1143 CCTTAGG
Asp700I 865 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1753 GAAAATGTTC
AvaI 121 CTCGGG C^[CT]CG[GA]G
1708 CTCGAG
AvaII 156 GGACC G^G[AT]CC
766 GGACC
BanI 7 GGCACC G^G[CT][GA]CC
926 GGCACC
BcnI 315 CCCGG CC^[GC]GG
773 CCCGG
BmyI 1210 GAGCAC G[AGT]GC[ACT]^C
2206 GGGCCC
BsaI 747 GAGACC GGTCTC(1/5)
1705 GGTCTC
BsaJI 314 CCCCGG C^C[ACGT][ACGT]GG
1031 CCCAGG
BsmBI 68 GAGACG CGTCTC(1/5)
545 GAGACG
BsmI 50 GAATGC GAATGC(1/-1)
1233 GAATGC
BsoBI 121 CTCGGG C^[CT]CG[GA]G
1708 CTCGAG
Bsp1286I 1210 GAGCAC G[AGT]GC[ACT]^C
2206 GGGCCC
BstBI 885 TTCGAA TT^CGAA
2089 TTCGAA
BstNI 159 CCTGG CC^[AT]GG
1032 CCAGG
Bsu36I 1126 CCTGAGG CC^T[ACGT]AGG
1143 CCTTAGG
Cac8I 100 GCCGGC GC[ACGT]^[ACGT]GC
759 GCGAGC
CfoI 242 GCGC GCG^C
780 GCGC
Eco81I 1126 CCTGAGG CC^T[ACGT]AGG
1143 CCTTAGG
EcoNI 1548 CCTTGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1572 CCTCAACAAGG
EcoRII 159 CCTGG ^CC[AT]GG
1032 CCAGG
HhaI 242 GCGC GCG^C
780 GCGC
HinP1I 242 GCGC G^CGC
780 GCGC
HphI 82 GGTGA GGTGA(8/7)
1996 GGTGA
MvaI 159 CCTGG CC^[AT]GG
1032 CCAGG
NciI 315 CCCGG CC^[GC]GG
773 CCCGG
NspV 885 TTCGAA TT^CGAA
2089 TTCGAA
SfuI 885 TTCGAA TT^CGAA
2089 TTCGAA
XmnI 865 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1753 GAAAATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbsI BbvI BfaI BpuAI BseRI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstF5I BstYI Cfr13I DdeI
DpnII DraII EarI EcoO109I Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI Ksp632I MaeI MboI MboII
MflI MnlI MseI MslI MspI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII