Restriction map for
pFN21AE2745
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BfrI BglI
BglII BlnI BpmI BsaAI BsaBI
BsaHI BseRI BsiWI BsmBI Bsp1407I
BspDI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MflI MluI MseI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
Van91I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 849 CGCG CG^CG
AcsI 23 GAATTC [GA]^AATT[CT]
AfaI 297 GTAC GT^AC
AlwNI 208 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 23 GAATTC [GA]^AATT[CT]
BalI 191 TGGCCA TGG^CCA
BbsI 610 GAAGAC GAAGAC(2/6)
BbvCI 348 CCTCAGC CCTCAGC(-5/-2)
BclI 555 TGATCA T^GATCA
BfaI 412 CTAG C^TAG
BlpI 489 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 489 GCTGAGC GC^T[ACGT]AGC
BpuAI 610 GAAGAC GAAGAC(2/6)
BsiEI 1018 CGGCCG CG[GA][CT]^CG
BsmI 401 GAATGC GAATGC(1/-1)
BspHI 173 TCATGA T^CATGA
BsrBI 98 GAGCGG CCGCTC(-3/-3)
BsrFI 503 GCCGGC [GA]^CCGG[CT]
BstAPI 510 GCAGCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 849 CGCG CG^CG
CelII 489 GCTGAGC GC^T[ACGT]AGC
Cfr10I 503 GCCGGC [GA]^CCGG[CT]
DsaI 119 CCACGG C^C[GA][CT]GG
EagI 1018 CGGCCG C^GGCCG
EclXI 1018 CGGCCG C^GGCCG
Eco52I 1018 CGGCCG C^GGCCG
EcoRI 23 GAATTC G^AATTC
EcoT14I 392 CCAAGG C^C[AT][AT]GG
FbaI 555 TGATCA T^GATCA
HgaI 847 GACGC GACGC(5/10)
MaeI 412 CTAG C^TAG
MluNI 191 TGGCCA TGG^CCA
MscI 191 TGGCCA TGG^CCA
MvnI 849 CGCG CG^CG
NaeI 503 GCCGGC GCC^GGC
NgoMIV 503 GCCGGC G^CCGGC
PstI 214 CTGCAG CTGCA^G
RcaI 173 TCATGA T^CATGA
RsaI 297 GTAC GT^AC
SfcI 214 CTGCAG C^T[GA][CT]AG
SgrAI 502 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 667 CTCAAG C^T[CT][GA]AG
SphI 1083 GCATGC GCATG^C
StyI 392 CCAAGG C^C[AT][AT]GG
Tsp509I 24 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 743 TCCGGA T^CCGGA
971 TCCGGA
AocI 577 CCTGAGG CC^T[ACGT]AGG
772 CCTGAGG
AvaI 632 CCCGGG C^[CT]CG[GA]G
1121 CCCGGG
BsaI 640 GAGACC GGTCTC(1/5)
1069 GAGACC
BseAI 743 TCCGGA T^CCGGA
971 TCCGGA
BsoBI 632 CCCGGG C^[CT]CG[GA]G
1121 CCCGGG
BspEI 743 TCCGGA T^CCGGA
971 TCCGGA
BspMI 263 ACCTGC ACCTGC(4/8)
368 ACCTGC
BsrI 680 CCAGT ACTGG(1/-1)
929 CCAGT
Bsu36I 577 CCTGAGG CC^T[ACGT]AGG
772 CCTGAGG
DraIII 109 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
1001 CACAGGGTG
EaeI 191 TGGCCA [CT]^GGCC[GA]
1018 CGGCCG
EarI 751 CTCTTC CTCTTC(1/4)
877 GAAGAG
Eco81I 577 CCTGAGG CC^T[ACGT]AGG
772 CCTGAGG
FauI 500 CCCGC CCCGC(4/6)
327 GCGGG
Ksp632I 751 CTCTTC CTCTTC(1/4)
877 GAAGAG
MroI 743 TCCGGA T^CCGGA
971 TCCGGA
NspI 515 ACATGC [GA]CATG^[CT]
1083 GCATGC
PpuMI 365 GGGACCT [GA]G^G[AT]CC[CT]
564 GGGTCCC
SfaNI 236 GCATC GCATC(5/9)
734 GCATC
SmaI 632 CCCGGG CCC^GGG
1121 CCCGGG
XcmI 431 CCATGATTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1101 CCACTCCCGTCATGG
XmaCI 632 CCCGGG C^CCGGG
1121 CCCGGG
XmaI 632 CCCGGG C^CCGGG
1121 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AluI ApaI AspHI AvaII
BanII BbvI BcnI BmyI BsaJI
BsaWI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PvuII SacI
Sau3AI Sau96I ScrFI TaqI TfiI
TseI Tsp45I TspRI TthHB8I