Restriction map for
pFN21AE1648
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaII AviII AvrII BalI BamHI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BseAI BsiHKAI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraI DraIII DrdI
DsaI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FspI HincII
HindII HindIII HinfI HpaI KpnI
Ksp632I KspI MaeI MaeII MamI
MboI MboII MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NcoI NdeI NdeII NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AlwNI 165 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 160 GGGCCC GGGCC^C
BanI 453 GGCGCC G^G[CT][GA]CC
BbeI 453 GGCGCC GGCGC^C
BbvCI 208 GCTGAGG CCTCAGC(-5/-2)
BpmI 106 CTGGAG CTGGAG(16/14)
BsaWI 383 ACCGGA [AT]^CCGG[AT]
BsgI 439 GTGCAG GTGCAG(16/14)
BsmAI 114 GAGAC GTCTC(1/5)
BspMI 239 ACCTGC ACCTGC(4/8)
BsrBI 279 GAGCGG CCGCTC(-3/-3)
BsrFI 411 GCCGGC [GA]^CCGG[CT]
BstAPI 167 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 395 CCACGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 411 GCCGGC [GA]^CCGG[CT]
DraII 418 GGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 413 CGGCCG C^GGCCG
EclXI 413 CGGCCG C^GGCCG
Eco52I 413 CGGCCG C^GGCCG
EcoO109I 418 GGGGCCT [GA]G^G[ACGT]CC[CT]
FseI 410 GGCCGGCC GGCCGG^CC
HgaI 484 GACGC GACGC(5/10)
HphI 360 TCACC GGTGA(8/7)
KasI 453 GGCGCC G^GCGCC
MaeIII 331 GTGAC ^GT[ACGT]AC
NaeI 411 GCCGGC GCC^GGC
NarI 453 GGCGCC GG^CGCC
NgoMIV 411 GCCGGC G^CCGGC
SfaNI 167 GCATC GCATC(5/9)
SfoI 453 GGCGCC GGC^GCC
SmaI 136 CCCGGG CCC^GGG
TaqI 467 TCGA T^CGA
Tsp45I 331 GTGAC ^GT[GC]AC
TspRI 118 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 467 TCGA T^CGA
XcmI 95 CCATGTCCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 136 CCCGGG C^CCGGG
XmaI 136 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcyI 453 GGCGCC G[GA]^CG[CT]C
484 GACGCC
BsaHI 453 GGCGCC G[GA]^CG[CT]C
484 GACGCC
BseRI 48 CTCCTC GAGGAG(10/8)
229 GAGGAG
BsiEI 381 CGACCG CG[GA][CT]^CG
413 CGGCCG
BsiYI 76 CCGAGGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
377 CCAGCGACCGG
BslI 76 CCGAGGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
377 CCAGCGACCGG
BsrI 39 ACTGG ACTGG(1/-1)
255 CCAGT
BstNI 105 CCTGG CC^[AT]GG
225 CCAGG
DdeI 112 CTGAG C^T[ACGT]AG
209 CTGAG
EcoRII 105 CCTGG ^CC[AT]GG
225 CCAGG
HaeII 213 GGCGCT [GA]GCGC^[CT]
453 GGCGCC
Hin1I 453 GGCGCC G[GA]^CG[CT]C
484 GACGCC
MvaI 105 CCTGG CC^[AT]GG
225 CCAGG
NlaIII 12 CATG CATG^
96 CATG
PvuII 400 CAGCTG CAG^CTG
442 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AluI AvaI BanII
BbvI BcnI BglI BmyI BsaJI
BsoBI Bsp1286I BssKI BstUI Cac8I
CfoI Cfr13I EaeI FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
ItaI MnlI MspA1I MspI MvnI
MwoI NciI NlaIV Sau96I ScrFI
TseI