Restriction map for
pFN21AE5292
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflIII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AviII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BcnI BglI
BglII BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT22I FauI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 283 GAATTT [GA]^AATT[CT]
AflII 154 CTTAAG C^TTAAG
AhdI 521 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 246 CCTGAGG CC^T[ACGT]AGG
ApoI 283 GAATTT [GA]^AATT[CT]
AspEI 521 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 570 GGACC G^G[AT]CC
AvrII 654 CCTAGG C^CTAGG
BclI 596 TGATCA T^GATCA
BfrI 154 CTTAAG C^TTAAG
BlnI 654 CCTAGG C^CTAGG
BsaJI 654 CCTAGG C^C[ACGT][ACGT]GG
BseRI 249 GAGGAG GAGGAG(10/8)
BsiYI 62 CCAAAACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 62 CCAAAACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI 617 ACCTGC ACCTGC(4/8)
BsrI 374 CCAGT ACTGG(1/-1)
BstYI 650 AGATCC [GA]^GATC[CT]
Bsu36I 246 CCTGAGG CC^T[ACGT]AGG
Cfr13I 570 GGACC G^G[ACGT]CC
DraII 569 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 521 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 246 CCTGAGG CC^T[ACGT]AGG
EcoO109I 569 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 159 GATATC GAT^ATC
EcoT14I 654 CCTAGG C^C[AT][AT]GG
FbaI 596 TGATCA T^GATCA
HaeIII 295 GGCC GG^CC
HgaI 354 GCGTC GACGC(5/10)
HincII 533 GTCAAC GT[CT]^[GA]AC
HindII 533 GTCAAC GT[CT]^[GA]AC
HindIII 148 AAGCTT A^AGCTT
MflI 650 AGATCC [GA]^GATC[CT]
MslI 670 CACGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 592 CAGCTG C[AC]G^C[GT]G
PpuMI 569 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 592 CAGCTG CAG^CTG
Sau96I 570 GGACC G^G[ACGT]CC
SmlI 154 CTTAAG C^T[CT][GA]AG
SspI 476 AATATT AAT^ATT
StyI 654 CCTAGG C^C[AT][AT]GG
TaqI 519 TCGA T^CGA
TspRI 498 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 519 TCGA T^CGA
XhoII 650 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 651 GATCC GGATC(4/5)
210 GGATC
AlwNI 241 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
330 CAGGAACTG
BbsI 14 GAAGAC GAAGAC(2/6)
319 GAAGAC
BfaI 202 CTAG C^TAG
655 CTAG
BpmI 608 CTCCAG CTGGAG(16/14)
298 CTGGAG
BpuAI 14 GAAGAC GAAGAC(2/6)
319 GAAGAC
BsmAI 252 GAGAC GTCTC(1/5)
401 GTCTC
BssKI 68 CCAGG ^CC[ACGT]GG
297 CCTGG
BstNI 68 CCAGG CC^[AT]GG
297 CCTGG
Cac8I 438 GCCTGC GC[ACGT]^[ACGT]GC
669 GCACGC
EcoRII 68 CCAGG ^CC[AT]GG
297 CCTGG
HphI 575 TCACC GGTGA(8/7)
635 TCACC
MaeI 202 CTAG C^TAG
655 CTAG
MseI 155 TTAA T^TAA
176 TTAA
MvaI 68 CCAGG CC^[AT]GG
297 CCTGG
NlaIII 588 CATG CATG^
625 CATG
ScrFI 68 CCAGG CC^[ACGT]GG
297 CCTGG
TfiI 195 GAATC G^A[AT]TC
661 GATTC
Tru9I 155 TTAA T^TAA
176 TTAA
Tsp509I 168 AATT ^AATT
284 AATT
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
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AluI BbvI BstF5I DdeI DpnII
Fnu4HI FokI HinfI ItaI MboI
MboII MnlI NdeII PleI PstI
Sau3AI SfaNI SfcI TseI