Restriction map for
pFN21AE5750
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbrPI BbsI BcgI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfuI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AcyI 79 GGCGCC G[GA]^CG[CT]C
BamHI 546 GGATCC G^GATCC
BbeI 79 GGCGCC GGCGC^C
BciVI 271 GGATAC GTATCC(6/5)
BclI 416 TGATCA T^GATCA
BglII 494 AGATCT A^GATCT
BsaAI 727 TACGTG [CT]AC^GT[GA]
BsaHI 79 GGCGCC G[GA]^CG[CT]C
BsaI 177 GGTCTC GGTCTC(1/5)
BsmBI 706 CGTCTC CGTCTC(1/5)
Bsp1407I 641 TGTACA T^GTACA
BspHI 1986 TCATGA T^CATGA
BsrBI 793 GAGCGG CCGCTC(-3/-3)
BsrDI 337 CATTGC GCAATG(2/0)
BsrGI 641 TGTACA T^GTACA
BssSI 490 CACGAG CACGAG(-5/-1)
EcoNI 1157 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 416 TGATCA T^GATCA
FseI 1791 GGCCGGCC GGCCGG^CC
Hin1I 79 GGCGCC G[GA]^CG[CT]C
KasI 79 GGCGCC G^GCGCC
NarI 79 GGCGCC GG^CGCC
NspI 1192 GCATGT [GA]CATG^[CT]
RcaI 1986 TCATGA T^CATGA
SfiI 1791 GGCCGGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 79 GGCGCC GGC^GCC
SgrAI 887 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 235 CTTGAG C^T[CT][GA]AG
SspBI 641 TGTACA T^GTACA
StuI 1256 AGGCCT AGG^CCT
TaqI 181 TCGA T^CGA
TthHB8I 181 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 630 GTAC GT^AC
642 GTAC
AlwNI 1557 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1946 CAGCCACTG
Aor51HI 1056 AGCGCT AGC^GCT
1592 AGCGCT
ApaI 1001 GGGCCC GGGCC^C
1938 GGGCCC
Asp700I 903 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1572 GAATGCCTTC
BbvCI 423 CCTCAGC CCTCAGC(-5/-2)
1761 CCTCAGC
BfaI 1435 CTAG C^TAG
1538 CTAG
BglI 205 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1792 GCCGGCCTGGC
BsmI 432 GAATGC GAATGC(1/-1)
1572 GAATGC
BspMI 394 ACCTGC ACCTGC(4/8)
1493 ACCTGC
BssHII 613 GCGCGC G^CGCGC
819 GCGCGC
BstAPI 1404 GCACATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1556 GCAGGCGCTGC
BstXI 722 CCAACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1928 CCATGGAGTTGG
Eco47III 1056 AGCGCT AGC^GCT
1592 AGCGCT
MaeI 1435 CTAG C^TAG
1538 CTAG
MaeII 728 ACGT A^CGT
1133 ACGT
MslI 756 CATTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1432 CACCTAGATG
NcoI 1073 CCATGG C^CATGG
1928 CCATGG
RsaI 630 GTAC GT^AC
642 GTAC
SacI 20 GAGCTC GAGCT^C
226 GAGCTC
SmaI 464 CCCGGG CCC^GGG
996 CCCGGG
XcmI 540 CCACAGGGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1478 CCAGTGCCCAGCTGG
XmaCI 464 CCCGGG C^CCGGG
996 CCCGGG
XmaI 464 CCCGGG C^CCGGG
996 CCCGGG
XmnI 903 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1572 GAATGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI Van91I
XhoII