Restriction map for
pFN21AE5280
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BbvI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstXI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI Ksp632I MaeI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TseI
Tsp45I Tsp509I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 242 GGTACC G^GTACC
AflIII 101 ACGTGT A^C[GA][CT]GT
AluI 318 AGCT AG^CT
ApaI 586 GGGCCC GGGCC^C
Asp718I 242 GGTACC G^GTACC
AspHI 317 GAGCTC G[AT]GC[AT]^C
AvaI 323 CCCGAG C^[CT]CG[GA]G
BanI 242 GGTACC G^G[CT][GA]CC
BbsI 202 GAAGAC GAAGAC(2/6)
BcgI 467 GCACTGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 570 CCGGG CC^[GC]GG
BglI 287 GCCGAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 71 CTCCAG CTGGAG(16/14)
BpuAI 202 GAAGAC GAAGAC(2/6)
BsaAI 55 TACGTG [CT]AC^GT[GA]
BsaBI 611 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 273 CGGCCG CG[GA][CT]^CG
BsiHKAI 317 GAGCTC G[AT]GC[AT]^C
BsmBI 194 GAGACG CGTCTC(1/5)
BsmFI 156 GGGAC GGGAC(10/14)
BsoBI 323 CCCGAG C^[CT]CG[GA]G
BspHI 546 TCATGA T^CATGA
BspMI 430 GCAGGT ACCTGC(4/8)
BstBI 515 TTCGAA TT^CGAA
BstNI 347 CCAGG CC^[AT]GG
CfoI 119 GCGC GCG^C
DdeI 533 CTAAG C^T[ACGT]AG
DraI 408 TTTAAA TTT^AAA
DraII 185 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 273 CGGCCG [CT]^GGCC[GA]
EagI 273 CGGCCG C^GGCCG
EclXI 273 CGGCCG C^GGCCG
Eco52I 273 CGGCCG C^GGCCG
EcoO109I 185 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 347 CCAGG ^CC[AT]GG
HaeII 118 GGCGCT [GA]GCGC^[CT]
HapII 570 CCGG C^CGG
HhaI 119 GCGC GCG^C
HinP1I 119 GCGC G^CGC
HpaII 570 CCGG C^CGG
KpnI 242 GGTACC GGTAC^C
KspI 150 CCGCGG CCGC^GG
MamI 611 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 150 CCGCGG C[AC]G^C[GT]G
MspI 570 CCGG C^CGG
MvaI 347 CCAGG CC^[AT]GG
NciI 570 CCGGG CC^[GC]GG
NruI 219 TCGCGA TCG^CGA
NspI 419 ACATGC [GA]CATG^[CT]
NspV 515 TTCGAA TT^CGAA
PflMI 598 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 666 GAGTC GAGTC(4/5)
PpuMI 185 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 546 TCATGA T^CATGA
SacI 317 GAGCTC GAGCT^C
SacII 150 CCGCGG CCGC^GG
SfaNI 50 GCATC GCATC(5/9)
SfuI 515 TTCGAA TT^CGAA
SmlI 139 CTCAAG C^T[CT][GA]AG
TspRI 468 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 598 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 388 CCAAGTGGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 414 GGATC GGATC(4/5)
697 GGATC
AvaII 157 GGACC G^G[AT]CC
186 GGACC
BanII 317 GAGCTC G[GA]GC[CT]^C
586 GGGCCC
BmyI 317 GAGCTC G[AGT]GC[ACT]^C
586 GGGCCC
BsmAI 194 GAGAC GTCTC(1/5)
373 GTCTC
Bsp1286I 317 GAGCTC G[AGT]GC[ACT]^C
586 GGGCCC
BsrI 469 ACTGG ACTGG(1/-1)
522 ACTGG
BssKI 347 CCAGG ^CC[ACGT]GG
570 CCGGG
DsaI 150 CCGCGG C^C[GA][CT]GG
276 CCGTGG
EcoT14I 86 CCAAGG C^C[AT][AT]GG
682 CCAAGG
HgaI 196 GACGC GACGC(5/10)
205 GACGC
HphI 433 GGTGA GGTGA(8/7)
700 TCACC
MaeII 56 ACGT A^CGT
101 ACGT
MaeIII 62 GTAAC ^GT[ACGT]AC
674 GTTAC
MseI 409 TTAA T^TAA
575 TTAA
MslI 51 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
418 CACATGCGTG
NlaIII 420 CATG CATG^
547 CATG
ScrFI 347 CCAGG CC^[ACGT]GG
570 CCGGG
StyI 86 CCAAGG C^C[AT][AT]GG
682 CCAAGG
TfiI 18 GATTC G^A[AT]TC
144 GAATC
Tru9I 409 TTAA T^TAA
575 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AccII AciI AfaI BglII BsaJI
BsiYI BslI BstF5I BstUI BstYI
Cfr13I DpnII FauI Fnu4HI FokI
HaeIII HinfI ItaI MboI MboII
MflI MnlI MvnI MwoI NdeII
NlaIV RsaI Sau3AI Sau96I TaqI
TthHB8I XhoII