Restriction map for
pFN21AE2769
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI AspEI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstXI BstYI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HpaI KasI
KspI MaeI MamI MfeI MflI
MluI MroI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tsp509I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1356 GGTACC G^GTACC
AcyI 40 GGCGTC G[GA]^CG[CT]C
AfaI 1357 GTAC GT^AC
AflIII 1163 ACGTGT A^C[GA][CT]GT
Asp700I 1325 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1356 GGTACC G^GTACC
BalI 1055 TGGCCA TGG^CCA
BbsI 73 GTCTTC GAAGAC(2/6)
BpuAI 73 GTCTTC GAAGAC(2/6)
BsaAI 1162 TACGTG [CT]AC^GT[GA]
BsaHI 40 GGCGTC G[GA]^CG[CT]C
BsaI 161 GGTCTC GGTCTC(1/5)
BseRI 328 GAGGAG GAGGAG(10/8)
BsmI 605 GAATGC GAATGC(1/-1)
BsrBI 1176 GAGCGG CCGCTC(-3/-3)
BstBI 808 TTCGAA TT^CGAA
DpnII 852 GATC ^GATC
DraII 4 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 1055 TGGCCA [CT]^GGCC[GA]
EcoNI 440 CCTCTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 4 AGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 1066 CCCGC CCCGC(4/6)
Hin1I 40 GGCGTC G[GA]^CG[CT]C
HincII 630 GTCAAC GT[CT]^[GA]AC
HindII 630 GTCAAC GT[CT]^[GA]AC
HindIII 1326 AAGCTT A^AGCTT
KpnI 1356 GGTACC GGTAC^C
MboI 852 GATC ^GATC
MluNI 1055 TGGCCA TGG^CCA
MscI 1055 TGGCCA TGG^CCA
MslI 458 CACAATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 395 CCATGG C^CATGG
NdeII 852 GATC ^GATC
NspV 808 TTCGAA TT^CGAA
PflMI 967 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 4 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1045 CAGCTG CAG^CTG
RsaI 1357 GTAC GT^AC
Sau3AI 852 GATC ^GATC
SfcI 166 CTACAG C^T[GA][CT]AG
SfuI 808 TTCGAA TT^CGAA
StuI 819 AGGCCT AGG^CCT
TaqI 809 TCGA T^CGA
TthHB8I 809 TCGA T^CGA
Van91I 967 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1325 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 169 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1042 CAGCAGCTG
AocI 676 CCTAAGG CC^T[ACGT]AGG
691 CCTCAGG
AspHI 985 GAGCTC G[AT]GC[AT]^C
1181 GAGCTC
AspI 534 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1254 GACCCAGTC
BglI 123 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
310 GCCAACCAGGC
BsaWI 289 ACCGGA [AT]^CCGG[AT]
1079 TCCGGT
BsiHKAI 985 GAGCTC G[AT]GC[AT]^C
1181 GAGCTC
BstAPI 600 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1041 GCAGCAGCTGC
Bsu36I 676 CCTAAGG CC^T[ACGT]AGG
691 CCTCAGG
DsaI 395 CCATGG C^C[GA][CT]GG
1097 CCGTGG
Eco81I 676 CCTAAGG CC^T[ACGT]AGG
691 CCTCAGG
MaeII 1163 ACGT A^CGT
1295 ACGT
MspA1I 1045 CAGCTG C[AC]G^C[GT]G
1067 CCGCTG
PflFI 534 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1254 GACCCAGTC
SacI 985 GAGCTC GAGCT^C
1181 GAGCTC
SapI 372 GAAGAGC GCTCTTC(1/4)
468 GAAGAGC
Tth111I 534 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1254 GACCCAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AvaII BanI BanII
BbvI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboII MnlI MseI MspI
MvaI MwoI NlaIII NlaIV PleI
Sau96I ScrFI SfaNI StyI Tru9I
TseI Tsp45I TspRI