Restriction map for
pFN21AE6659
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BcnI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Cac8I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeII MamI MluI MluNI MroI
MscI MspA1I MspI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
SspBI StuI SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 520 GAATTT [GA]^AATT[CT]
AflII 580 CTTAAG C^TTAAG
AlwNI 397 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 430 CCTCAGG CC^T[ACGT]AGG
ApoI 520 GAATTT [GA]^AATT[CT]
BanI 435 GGTGCC G^G[CT][GA]CC
BanII 230 GGGCTC G[GA]GC[CT]^C
BbvI 396 GCAGC GCAGC(8/12)
BclI 168 TGATCA T^GATCA
BfrI 580 CTTAAG C^TTAAG
BglII 750 AGATCT A^GATCT
BsaI 253 GAGACC GGTCTC(1/5)
BsaJI 140 CCATGG C^C[ACGT][ACGT]GG
BssKI 207 CCAGG ^CC[ACGT]GG
BstEII 333 GGTGACC G^GT[ACGT]ACC
BstNI 207 CCAGG CC^[AT]GG
BstPI 333 GGTGACC G^GT[ACGT]ACC
BstXI 257 CCACGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 430 CCTCAGG CC^T[ACGT]AGG
DsaI 140 CCATGG C^C[GA][CT]GG
EarI 460 GAAGAG CTCTTC(1/4)
Eco81I 430 CCTCAGG CC^T[ACGT]AGG
EcoO65I 333 GGTGACC G^GT[ACGT]ACC
EcoRII 207 CCAGG ^CC[AT]GG
EcoT14I 140 CCATGG C^C[AT][AT]GG
FbaI 168 TGATCA T^GATCA
Fnu4HI 396 GCAGC GC^[ACGT]GC
HaeIII 159 GGCC GG^CC
HgaI 377 GACGC GACGC(5/10)
HindIII 832 AAGCTT A^AGCTT
ItaI 396 GCAGC GC^[ACGT]GC
Ksp632I 460 GAAGAG CTCTTC(1/4)
MaeIII 334 GTGAC ^GT[ACGT]AC
MfeI 593 CAATTG C^AATTG
MslI 306 CACTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 593 CAATTG C^AATTG
MvaI 207 CCAGG CC^[AT]GG
NcoI 140 CCATGG C^CATGG
NlaIV 435 GGTGCC GG[ACGT]^[ACGT]CC
SbfI 327 CCTGCAGG CCTGCA^GG
ScrFI 207 CCAGG CC^[ACGT]GG
SmlI 580 CTTAAG C^T[CT][GA]AG
SpeI 652 ACTAGT A^CTAGT
Sse8387I 327 CCTGCAGG CCTGCA^GG
SspI 814 AATATT AAT^ATT
StyI 140 CCATGG C^C[AT][AT]GG
TseI 396 GCAGC G^C[AT]GC
Tsp45I 334 GTGAC ^GT[GC]AC
XbaI 480 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 225 GTAC GT^AC
504 GTAC
AluI 99 AGCT AG^CT
833 AGCT
AlwI 93 GATCC GGATC(4/5)
423 GGATC
BbsI 544 GAAGAC GAAGAC(2/6)
445 GTCTTC
BmyI 230 GGGCTC G[AGT]GC[ACT]^C
838 GTGCCC
BpuAI 544 GAAGAC GAAGAC(2/6)
445 GTCTTC
BsmAI 253 GAGAC GTCTC(1/5)
203 GTCTC
Bsp1286I 230 GGGCTC G[AGT]GC[ACT]^C
838 GTGCCC
BspMI 623 ACCTGC ACCTGC(4/8)
330 GCAGGT
BsrI 227 ACTGG ACTGG(1/-1)
307 ACTGG
BstYI 92 AGATCC [GA]^GATC[CT]
750 AGATCT
MflI 92 AGATCC [GA]^GATC[CT]
750 AGATCT
MwoI 429 GCCTCAGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
825 GCAGAGAAAGC
PleI 768 GACTC GAGTC(4/5)
607 GAGTC
PstI 328 CTGCAG CTGCA^G
625 CTGCAG
RsaI 225 GTAC GT^AC
504 GTAC
SfaNI 136 GATGC GCATC(5/9)
364 GATGC
SfcI 328 CTGCAG C^T[GA][CT]AG
625 CTGCAG
TaqI 362 TCGA T^CGA
588 TCGA
TspRI 306 CACTG CA[GC]TG[ACGT][ACGT]^
694 CACTG
TthHB8I 362 TCGA T^CGA
588 TCGA
XhoII 92 AGATCC [GA]^GATC[CT]
750 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
Asp700I BfaI BpmI BsiYI BslI
BstF5I DdeI DpnII FokI HinfI
HphI MaeI MboI MboII MnlI
MseI NdeII NlaIII Sau3AI TfiI
Tru9I Tsp509I XmnI