Restriction map for
pFN21AE2475
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AfaI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII BalI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfrI BglI BglII
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsgI BsiEI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV FseI FspI
HaeII Hin1I KasI KpnI Ksp632I
KspI MaeII MfeI MluI MluNI
MscI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 108 CGCG CG^CG
AccIII 744 TCCGGA T^CCGGA
AciI 832 GCGG CCGC(-3/-1)
Alw44I 431 GTGCAC G^TGCAC
ApaLI 431 GTGCAC G^TGCAC
AvrII 732 CCTAGG C^CTAGG
BamHI 75 GGATCC G^GATCC
BanII 783 GAGCCC G[GA]GC[CT]^C
BclI 170 TGATCA T^GATCA
BlnI 732 CCTAGG C^CTAGG
BsaBI 1031 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 744 TCCGGA [AT]^CCGG[AT]
BseAI 744 TCCGGA T^CCGGA
BsmFI 188 GGGAC GGGAC(10/14)
BspEI 744 TCCGGA T^CCGGA
BspHI 417 TCATGA T^CATGA
BsrFI 377 ACCGGC [GA]^CCGG[CT]
BstAPI 193 GCATGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 339 GGTGACC G^GT[ACGT]ACC
BstPI 339 GGTGACC G^GT[ACGT]ACC
BstUI 108 CGCG CG^CG
BstYI 75 GGATCC [GA]^GATC[CT]
CfoI 109 GCGC GCG^C
Cfr10I 377 ACCGGC [GA]^CCGG[CT]
DraII 132 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoNI 213 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 132 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 339 GGTGACC G^GT[ACGT]ACC
EcoT22I 692 ATGCAT ATGCA^T
FauI 832 GCGGG CCCGC(4/6)
FbaI 170 TGATCA T^GATCA
HgaI 190 GACGC GACGC(5/10)
HhaI 109 GCGC GCG^C
HinP1I 109 GCGC G^CGC
HincII 825 GTTAAC GT[CT]^[GA]AC
HindII 825 GTTAAC GT[CT]^[GA]AC
HindIII 925 AAGCTT A^AGCTT
HpaI 825 GTTAAC GTT^AAC
MamI 1031 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 75 GGATCC [GA]^GATC[CT]
MroI 744 TCCGGA T^CCGGA
MslI 405 CACTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 108 CGCG CG^CG
NsiI 692 ATGCAT ATGCA^T
PpuMI 132 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 644 CAGCTG CAG^CTG
RcaI 417 TCATGA T^CATGA
SexAI 153 ACCAGGT A^CC[AT]GGT
TaqI 680 TCGA T^CGA
TthHB8I 680 TCGA T^CGA
XhoII 75 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 76 GATCC GGATC(4/5)
75 GGATC
AspHI 431 GTGCAC G[AT]GC[AT]^C
845 GTGCTC
BfaI 284 CTAG C^TAG
733 CTAG
BseRI 756 CTCCTC GAGGAG(10/8)
550 GAGGAG
BsiHKAI 431 GTGCAC G[AT]GC[AT]^C
845 GTGCTC
BsrDI 13 GCAATG GCAATG(2/0)
1013 GCAATG
BstF5I 453 GGATG GGATG(2/0)
751 GGATG
FokI 453 GGATG GGATG(9/13)
751 GGATG
HapII 378 CCGG C^CGG
745 CCGG
HpaII 378 CCGG C^CGG
745 CCGG
MaeI 284 CTAG C^TAG
733 CTAG
MspA1I 644 CAGCTG C[AC]G^C[GT]G
830 CAGCGG
MspI 378 CCGG C^CGG
745 CCGG
NlaIV 75 GGATCC GG[ACGT]^[ACGT]CC
294 GGAGCC
NspI 143 ACATGC [GA]CATG^[CT]
841 GCATGT
PleI 457 GACTC GAGTC(4/5)
264 GAGTC
SmlI 848 CTCAAG C^T[CT][GA]AG
942 CTTGAG
TfiI 51 GAATC G^A[AT]TC
277 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AcsI AluI ApoI AvaII BbvI
BmyI BsaJI BsiYI BslI BsmI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII Sau3AI
Sau96I ScrFI SfaNI StyI Tru9I
TseI Tsp45I Tsp509I TspRI