##################################
# NO CUT ENZYMES (  195 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaII       AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaI        HphI        KasI        KpnI        
KspI        MaeI        MaeII       MaeIII      MamI        
MboI        MfeI        MflI        MluI        MroI        
MslI        MspA1I      MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        Sau3AI      SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tsp45I      Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AciI           25  CCGC                 CCGC(-3/-1)
AflII          97  CTTAAG               C^TTAAG
AlwNI          56  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI          184  CCCGGG               C^[CT]CG[GA]G
BalI          138  TGGCCA               TGG^CCA
BanI           92  GGCACC               G^G[CT][GA]CC
BbvI           76  GCTGC                GCAGC(8/12)
BfrI           97  CTTAAG               C^TTAAG
BmyI           45  GGGCAC               G[AGT]GC[ACT]^C
BsiYI          43  CCGGGCACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           43  CCGGGCACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI         184  CCCGGG               C^[CT]CG[GA]G
Bsp1286I       45  GGGCAC               G[AGT]GC[ACT]^C
BsrI           49  ACTGG                ACTGG(1/-1)
BstNI         224  CCTGG                CC^[AT]GG
Cac8I          27  GCCTGC               GC[ACGT]^[ACGT]GC
Cfr13I        182  GGCCC                G^G[ACGT]CC
DdeI           62  CTGAG                C^T[ACGT]AG
EaeI          138  TGGCCA               [CT]^GGCC[GA]
EarI          286  GAAGAG               CTCTTC(1/4)
EcoRII        224  CCTGG                ^CC[AT]GG
EcoT14I       248  CCAAGG               C^C[AT][AT]GG
FauI           24  CCCGC                CCCGC(4/6)
Fnu4HI         76  GCTGC                GC^[ACGT]GC
HindIII       266  AAGCTT               A^AGCTT
ItaI           76  GCTGC                GC^[ACGT]GC
Ksp632I       286  GAAGAG               CTCTTC(1/4)
MluNI         138  TGGCCA               TGG^CCA
MscI          138  TGGCCA               TGG^CCA
MseI           98  TTAA                 T^TAA
MvaI          224  CCTGG                CC^[AT]GG
NlaIII        261  CATG                 CATG^
PstI           77  CTGCAG               CTGCA^G
SapI          286  GAAGAGC              GCTCTTC(1/4)
Sau96I        182  GGCCC                G^G[ACGT]CC
SfcI           77  CTGCAG               C^T[GA][CT]AG
SmaI          184  CCCGGG               CCC^GGG
SmlI           97  CTTAAG               C^T[CT][GA]AG
StyI          248  CCAAGG               C^C[AT][AT]GG
Tru9I          98  TTAA                 T^TAA
TseI           76  GCTGC                G^C[AT]GC
TspRI          48  CACTG                CA[GC]TG[ACGT][ACGT]^
XmaCI         184  CCCGGG               C^CCGGG
XmaI          184  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BsaJI         184  CCCGGG               C^C[ACGT][ACGT]GG
              248  CCAAGG              
BsmFI          52  GGGAC                GGGAC(10/14)
              241  GGGAC               
HapII          43  CCGG                 C^CGG
              185  CCGG                
HpaII          43  CCGG                 C^CGG
              185  CCGG                
MboII         253  GAAGA                GAAGA(8/7)
              286  GAAGA               
MspI           43  CCGG                 C^CGG
              185  CCGG                
MwoI           18  GCTCCTCCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              259  GCCATGAAAGC         
NlaIV          17  GGCTCC               GG[ACGT]^[ACGT]CC
               92  GGCACC              


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AluI        BcnI        BseRI       BssKI       BstF5I      
FokI        HaeIII      MnlI        NciI        ScrFI       
SfaNI