Restriction map for
pFN21AE6576
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MfeI MluI MroI MslI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tsp509I TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AlwI 225 GATCC GGATC(4/5)
AspHI 36 GTGCTC G[AT]GC[AT]^C
BalI 310 TGGCCA TGG^CCA
BcnI 145 CCGGG CC^[GC]GG
BsiHKAI 36 GTGCTC G[AT]GC[AT]^C
BsmFI 147 GGGAC GGGAC(10/14)
BspHI 23 TCATGA T^CATGA
BsrFI 213 GCCGGT [GA]^CCGG[CT]
BstAPI 299 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 191 GGATG GGATG(2/0)
BstYI 224 AGATCC [GA]^GATC[CT]
Cac8I 308 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 213 GCCGGT [GA]^CCGG[CT]
DdeI 221 CTCAG C^T[ACGT]AG
DpnII 225 GATC ^GATC
DraII 289 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 110 CCATGG C^C[GA][CT]GG
EaeI 310 TGGCCA [CT]^GGCC[GA]
EcoO109I 289 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 110 CCATGG C^C[AT][AT]GG
FokI 191 GGATG GGATG(9/13)
HinfI 149 GACTC G^A[ACGT]TC
MaeIII 100 GTCAC ^GT[ACGT]AC
MboI 225 GATC ^GATC
MboII 57 GAAGA GAAGA(8/7)
MflI 224 AGATCC [GA]^GATC[CT]
MluNI 310 TGGCCA TGG^CCA
MscI 310 TGGCCA TGG^CCA
MspA1I 303 CAGCTG C[AC]G^C[GT]G
NciI 145 CCGGG CC^[GC]GG
NcoI 110 CCATGG C^CATGG
NdeII 225 GATC ^GATC
PleI 149 GACTC GAGTC(4/5)
PstI 294 CTGCAG CTGCA^G
PvuII 303 CAGCTG CAG^CTG
RcaI 23 TCATGA T^CATGA
Sau3AI 225 GATC ^GATC
SfcI 294 CTGCAG C^T[GA][CT]AG
StyI 110 CCATGG C^C[AT][AT]GG
Tsp45I 100 GTCAC ^GT[GC]AC
XhoII 224 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BanI 91 GGTGCC G^G[CT][GA]CC
184 GGTGCC
BmyI 36 GTGCTC G[AGT]GC[ACT]^C
92 GTGCCC
BsaJI 95 CCCTGG C^C[ACGT][ACGT]GG
110 CCATGG
Bsp1286I 36 GTGCTC G[AGT]GC[ACT]^C
92 GTGCCC
BstNI 96 CCTGG CC^[AT]GG
188 CCTGG
EcoRII 96 CCTGG ^CC[AT]GG
188 CCTGG
HapII 145 CCGG C^CGG
214 CCGG
HpaII 145 CCGG C^CGG
214 CCGG
MseI 209 TTAA T^TAA
266 TTAA
MspI 145 CCGG C^CGG
214 CCGG
MvaI 96 CCTGG CC^[AT]GG
188 CCTGG
Tru9I 209 TTAA T^TAA
266 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI AluI AlwNI BbvI BsiYI
BslI BssKI Cfr13I Fnu4HI HaeIII
ItaI MnlI MwoI NlaIII NlaIV
Sau96I ScrFI TseI