Restriction map for
pFN21AE5189
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeI MaeII
MfeI MflI MluI MluNI MroI
MscI MseI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AlwI 323 GGATC GGATC(4/5)
AlwNI 147 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 68 GGTCC G^G[AT]CC
BpmI 274 CTGGAG CTGGAG(16/14)
BsaBI 133 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 166 GAGACC GGTCTC(1/5)
BsgI 77 CTGCAC GTGCAG(16/14)
BsiYI 331 CCTCACCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 331 CCTCACCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 166 GAGAC GTCTC(1/5)
BsmFI 69 GTCCC GGGAC(10/14)
BsmI 385 GAATGC GAATGC(1/-1)
BsrDI 260 GCAATG GCAATG(2/0)
Cac8I 341 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 144 GCGC GCG^C
DdeI 290 CTGAG C^T[ACGT]AG
DraII 269 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 112 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 83 CCATGG C^C[GA][CT]GG
EcoNI 331 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 269 AGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 248 GCGGG CCCGC(4/6)
HhaI 144 GCGC GCG^C
HinP1I 144 GCGC G^CGC
MaeIII 425 GTGAC ^GT[ACGT]AC
MamI 133 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 303 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 83 CCATGG C^CATGG
PleI 295 GAGTC GAGTC(4/5)
SfcI 367 CTACAG C^T[GA][CT]AG
TfiI 105 GAATC G^A[AT]TC
Tsp45I 425 GTGAC ^GT[GC]AC
TspRI 288 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 192 GTAC GT^AC
210 GTAC
AluI 148 AGCT AG^CT
361 AGCT
BmyI 232 GTGCCC G[AGT]GC[ACT]^C
283 GTGCCC
BseRI 95 GAGGAG GAGGAG(10/8)
292 GAGGAG
Bsp1286I 232 GTGCCC G[AGT]GC[ACT]^C
283 GTGCCC
BstAPI 146 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
312 GCAGATTATGC
Cfr13I 68 GGTCC G^G[ACGT]CC
270 GGGCC
DpnII 204 GATC ^GATC
324 GATC
EcoT14I 83 CCATGG C^C[AT][AT]GG
336 CCAAGG
HaeIII 271 GGCC GG^CC
340 GGCC
HinfI 105 GAATC G^A[ACGT]TC
295 GAGTC
MboI 204 GATC ^GATC
324 GATC
MboII 182 TCTTC GAAGA(8/7)
215 TCTTC
NdeII 204 GATC ^GATC
324 GATC
NlaIV 29 GGAGCC GG[ACGT]^[ACGT]CC
68 GGTCCC
RsaI 192 GTAC GT^AC
210 GTAC
Sau3AI 204 GATC ^GATC
324 GATC
Sau96I 68 GGTCC G^G[ACGT]CC
270 GGGCC
SfaNI 240 GATGC GCATC(5/9)
245 GATGC
StyI 83 CCATGG C^C[AT][AT]GG
336 CCAAGG
TaqI 164 TCGA T^CGA
206 TCGA
TthHB8I 164 TCGA T^CGA
206 TCGA
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AciI BbvI BsaJI BssKI BstF5I
BstNI EcoRII Fnu4HI FokI HphI
ItaI MnlI MvaI MwoI NlaIII
ScrFI TseI