Restriction map for
pFN21AE5186
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# NO CUT ENZYMES ( 204 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AgeI AhdI
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstXI BstYI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DdeI DraI DraII DraIII DrdI
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HinfI HpaI HphI
KasI KpnI KspI MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MslI MspA1I MunI MvaI
MwoI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TaqI TfiI Tsp45I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AccII 12 CGCG CG^CG
AcsI 205 AAATTT [GA]^AATT[CT]
AflIII 151 ACACGT A^C[GA][CT]GT
ApoI 205 AAATTT [GA]^AATT[CT]
BbvI 71 GCAGC GCAGC(8/12)
BfaI 52 CTAG C^TAG
BsaJI 56 CCCCGG C^C[ACGT][ACGT]GG
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsiYI 51 CCTAGCCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 51 CCTAGCCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BssSI 91 CACGAG CACGAG(-5/-1)
BstUI 12 CGCG CG^CG
DpnII 160 GATC ^GATC
EaeI 8 CGGCCG [CT]^GGCC[GA]
EagI 8 CGGCCG C^GGCCG
EarI 176 GAAGAG CTCTTC(1/4)
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
Ksp632I 176 GAAGAG CTCTTC(1/4)
MaeI 52 CTAG C^TAG
MboI 160 GATC ^GATC
MseI 31 TTAA T^TAA
MvnI 12 CGCG CG^CG
NdeII 160 GATC ^GATC
Sau3AI 160 GATC ^GATC
SspI 107 AATATT AAT^ATT
Tru9I 31 TTAA T^TAA
TseI 71 GCAGC G^C[AT]GC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaII 42 GGACC G^G[AT]CC
87 GGACC
BcnI 39 CCGGG CC^[GC]GG
57 CCCGG
BseRI 179 GAGGAG GAGGAG(10/8)
232 GAGGAG
BsmFI 41 GGGAC GGGAC(10/14)
86 GGGAC
BssKI 39 CCGGG ^CC[ACGT]GG
57 CCCGG
BstF5I 24 GGATG GGATG(2/0)
227 GGATG
CfoI 13 GCGC GCG^C
69 GCGC
Fnu4HI 10 GCCGC GC^[ACGT]GC
71 GCAGC
FokI 24 GGATG GGATG(9/13)
227 GGATG
HaeIII 9 GGCC GG^CC
37 GGCC
HapII 39 CCGG C^CGG
58 CCGG
HhaI 13 GCGC GCG^C
69 GCGC
HinP1I 13 GCGC G^CGC
69 GCGC
HpaII 39 CCGG C^CGG
58 CCGG
ItaI 10 GCCGC GC^[ACGT]GC
71 GCAGC
MaeII 77 ACGT A^CGT
153 ACGT
MboII 176 GAAGA GAAGA(8/7)
214 GAAGA
MnlI 179 GAGG CCTC(7/6)
232 GAGG
MspI 39 CCGG C^CGG
58 CCGG
NciI 39 CCGGG CC^[GC]GG
57 CCCGG
NlaIV 41 GGGACC GG[ACGT]^[ACGT]CC
86 GGGACC
ScrFI 39 CCGGG CC^[ACGT]GG
57 CCCGG
Tsp509I 137 AATT ^AATT
206 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
AciI Cfr13I Sau96I